New nor-ursane type triterpenoids from Gelsemium elegans

Wu, Huifeng; He, Xingxing; Jin, Xiaojie; Pan, Hong; Shi, Zhuan-Ning; Xu, Dongdong; Yao, Xiaojun; Zhu, Yunlong

doi:10.1016/j.fitote.2015.09.002

Cited by 15 publications

(2 citation statements)

References 40 publications

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“…Gelsemium elegans is distributed in Southeast Asia, especially in China, and the other two species are predominant in North America (Xu et al 2006) . It has been well studied that Gelsemium extract and its active alkaloids have a variety of biological functions, such as anti-tumor, anti-cancer, anti-inflammatory, anti-cytotoxicity, antiepidermal cell proliferation, anti-arrhythmic, analgesic and immunomodulating activities (Kitajima et al 2003;Kogure et al 2006;Xu et al 2012;Qu et al 2013;Wu et al 2015;Zhang and Wang 2015). Our study can be used in the study of population genetics and evolutionary studies, and contribute to future related research.…”

Section: Chloroplast Genome; Gelsemium Elegans;mentioning

confidence: 81%

Complete sequence and comparative analysis of the chloroplast genome of Gelsemium elegans

Wang

Liu

Sun

et al. 2019

Mitochondrial DNA Part B

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In this study, we show the complete chloroplast genome in G. elegans by high-throughput sequencing. The chloroplast genome in G. elegans is 155,018 bp in size, comprising a pair of inverted repeats (IRa and IRb) of 25,728 bp each and an LSC region of 85,619 bp and an SSC region of 17,943 bp. The genome includes 117 single-copy genes (29 tRNA, 8 rRNA genes and 80 protein-coding). Phylogenetic analysis using total chloroplast genome DNA sequence of 15 species belonging to Gentianales showed that Apocynaceae and Loganiaceae form a single cluster with closer relationship to Gentianaceae than with Rubiaceae.

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Section: Chloroplast Genome; Gelsemium Elegans;mentioning

confidence: 81%

Complete sequence and comparative analysis of the chloroplast genome of Gelsemium elegans

Wang

Liu

Sun

et al. 2019

Mitochondrial DNA Part B

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“…At present, >40 indole alkaloids have been isolated, of which gelsenicine has the strongest toxicity (Kogure, Ishii, Kitajima, Wongseripipatana, & Takayama, ; Liu et al, ). In recent years, the role of G. elegans Benth preparations in anti‐tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use (Liu et al, ; Wu et al, ; Xu et al, ). Therefore, it is necessary to establish an analytical method to monitor the concentration of gelsenicine.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Jin

et al. 2018

Biomedical Chromatography

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Gelsenicine is an indole alkaloid isolated from Gelsemium elegans Benth. In recent years, the role of G. elegans Benth preparations in anti-tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use. An UPLC-MS/MS method was established to determine the gelsenicine in mouse blood, and the pharmacokinetics of gelsenicine after intravenous (0.1 mg/kg) and intragastric (0.5 and 1 mg/kg) administration was studied. Deltalin was used as internal standard; a UPLC BEH C 18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of gelsenicine in electrospray ionization positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. A validation of this method was performed in accordance with the US Food and Drug Administration guidelines. In the concentration range of 0.05-100 ng/mL, the gelsenicine in the mouse blood was linear (r > 0.995), and the lower limit of quantification was 0.05 ng/mL. In the mouse blood, the intra-day precision RSD was <12%, the inter-day precision RSD was <15%, the accuracy ranged from 89.8 to 112.3%, the average recovery was >76.8%, and the matrix effect was between 103.7 and 108.4%, which meet the pharmacokinetic research requirements of gelsenicine. The UPLC-MS/MS method is sensitive, rapid and selective, and has been successfully applied to the pharmacokinetic study of gelsenicine in mice. The absolute bioavailability of gelsenicine is 1.13%.

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