New Pineapple Somaclonal Variants: P3R5 and Dwarf

Pérez, Gabriel Sosa; Yanez, Ermis; Mbogholi, Andrew; Valle, Bárbara; Sagarra, Fernando; Yabor, Lourdes; Aragón, C.; González, José Ignacio López; Isidrón, Miriam; Lorenzo, José Carlos

doi:10.4236/ajps.2012.31001

Cited by 25 publications

(12 citation statements)

References 48 publications

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“…)Spineless variantJaya et al (2002)Cvs. P3R5 and Dwarf, variation in fruit color, growth habit, fruit size and length of plant generation cyclePérez et al (2009, 2012)43Potato ( Solanum tuberosum L.)Non-browning var. White BaronArihara et al (1995)Somaclones for heat toleranceDas et al (2000)Somaclones IBP-10, IBP-27 and IBP-30, derived from cultivar Desiree, showed higher resistance to Alternaria solani and Streptomyces scabiei Veitia-Rodriguez et al (2002)Improved size, shape, appearance, starch content and starch yieldThieme and Griess (2005)Superior processing attributes than cv.…”

Section: Application Of Somaclonal Variationsmentioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

341

216

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The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Therefore, it is of immense significance to assure the genetic uniformity of in vitro raised plants at an early stage. Several strategies have been followed to ascertain the genetic fidelity of the in vitro raised progenies comprising morpho-physiological, biochemical, cytological and DNA-based molecular markers approaches. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in horticultural crops, which are either difficult to breed or have narrow genetic base. In the present paper, sources of variations induced during tissue culture cycle and strategies to ascertain and confirm genetic fidelity in a variety of in vitro raised plantlets and potential application of variants in horticultural crop improvement are reviewed.

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Section: Application Of Somaclonal Variationsmentioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

341

216

View full text Add to dashboard Cite

show abstract

“…AFLP analysis is commonly used to assess the genetic conformity of plants regenerated by in vitro culture (Aversano et al 2011;Mehta et al 2011) or to detect somaclonal variations (Mo et al 2009;Perez et al 2012). An analysis was performed on the rhizome-propagated plants, short-term culture (ST) in vitro-propagated plants (''vitroplants'') and long-term culture (LT) in vitro-propagated plants.…”

Section: Aflp Analysismentioning

confidence: 99%

Shoot organogenesis in three Miscanthus species and evaluation for genetic uniformity using AFLP analysis

Rambaud

Arnoult²,

Bluteau

et al. 2013

Plant Cell Tiss Organ Cult

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A simple, efficient protocol for direct in vitro shoot organogenesis and regeneration was established for three species of Miscanthus including two clones of Miscanthus x giganteus, one clone of M. sinensis and one clone of M. sacchariflorus. Shoots were induced from the axillary nodes of both M. x giganteus and M. sacchariflorus and from apical meristems of both M. sinensis and M. sacchariflorus. A tillering method was used to accelerate shoot proliferation. Shoots were rooted in a wet perlite substrate in pots in the greenhouse. Subsequently, rooted plants were transferred to the field. The genetic uniformity of regenerated plants was evaluated using amplified fragment length polymorphism analysis and compared to that of rhizome-propagated plants. A total of 33,443 fragments were generated, representing 869 markers. There were 21 fragments (0.06 % of the fragments) or 19 markers (2.19 % of the markers) that were polymorphic, and almost all of these were singletons. The three species showed similar polymorphisms. Genetic variability was also found in the rhizome-propagated plants, sometimes at a higher rate than in the in vitro culture, indicating that the genetic uniformity was not altered by the protocol. This protocol may help breeders produce new clones of Miscanthus in the future.

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“…Table 1 shows the effects of the second experiment. Electrolyte leakage and chlorophyll b concentration were the most modified indicators by the different times of seed exposure performed a comprehensive literature search, we have not found any procedure similar and simpler to the one described in this paper, except for our previous publications (Cejas et al, 2012;Gomez-Pando et al, 2009;González et al, 2010;González et al, 2013;Pérez et al, 2013;Pérez et al, 2012;Zevallos et al, 2013a;Zevallos et al, 2013b), not focused on the use of statistical or mathematical methods, just on the results.…”

Section: Resultsmentioning

confidence: 76%

Coefficient of Variation Can Identify the Most Important Effects of Experimental Treatments

Lorenzo

Yabor

Medina

et al. 2015

Not Bot Hort Agrobot Cluj

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Most agricultural experiments involve evaluation of multiple variables and at times it can be difficult to identify the biologically relevant effects of the experimental treatments after performing the traditional ANOVA, Tukey and t-tests. The coefficient of variation formula could be an important tool to focus 'Result and Discussion' sections only on the most important changes produced by the experimental treatments. This short report is intended to exemplify the use of the coefficient of variation in three plant physiology experiments. The first one dealt with the effects of common bean plantlet exposure to high temperature under controlled conditions (levels: 28 and 40 °C). The second experiment was related to common bean seed exposure to liquid nitrogen during five different periods of time (levels: 0, 7, 14, 21 and 28 days). The third experiment was bi-factorial: factor 1 was the 'type of plant material' (levels: pineapple plants genetically transformed and the untransformed control); and factor 2 was the 'time of in vitro-plantlet hardening' (levels: 0, 15 and 30 days). Contents of phenolics, aldehydes, chlorophylls and proteins were determined. Percentage of seed germination, electrolyte leakage, peroxidase activity, plant height and weight were also measured. Experiments were monofactorial with two levels, monofactorial with five levels and bifactorial, respectively, with randomized design. The coefficient of variation showed that the most remarkable effects of high temperature were recorded in free phenolics and chlorophylls (a, b, total). Electrolyte leakage and chlorophyll b concentration were the most modified indicators as a result of seed exposure to liquid nitrogen. In the third experiment, modification in the levels of malondialdehyde and other aldehydes were the most relevant changes resulting from factors interactions. A similar procedure has not been published, except for our previous publications, not focused on the use of the coefficient of variation, just on the biological results.

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New Pineapple Somaclonal Variants: P3R5 and Dwarf

Cited by 25 publications

References 48 publications

Somaclonal variations and their applications in horticultural crops improvement

Somaclonal variations and their applications in horticultural crops improvement

Shoot organogenesis in three Miscanthus species and evaluation for genetic uniformity using AFLP analysis

Coefficient of Variation Can Identify the Most Important Effects of Experimental Treatments

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