New planar chromatographic techniques in natural product analysis

Abstract

Planar chromatography (PLC) is gaining importance in the analysis of natural products. Sophisticated instrumentation has become available in this field. Major progress in planar chromatography has been achieved through the development of forced-flow techniques. Progress has also been made in the capillary-controlled flow techniques with the use of modified development chambers. A large variety of stationary phases is available and strategies to optimize mobile phase compositions in PLC have been developed. The detection and quantitation in PLC has improved through direct coupling of PLC with spectroscopic methods with and the use of modern, computerized scanning densitometers.

Preparative Layer Chromatography

“…The normal chamber was used in more than 95% of the separations, although efforts are being made to introduce separations by the CLC-5 technique (71). Although the new types of chambers (micro, ultramicro, and planar column) were introduced relatively recently, their efficiency has been demonstrated for various classes of substances (9,(82)(83)(84).…”