New possibilities for the glaucoma diagnostics using the synchronous fluorescence spectra of tear fluid

Krajčíková, Kristína; Skirková, Miriama; Moravská, Monika; Horňák, Marek; Firment, Peter; Dubayová, Katarı́na; Tomečková, Vladimı́ra

doi:10.1080/00387010.2021.2003406

Cited by 2 publications

(10 citation statements)

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“…The paper presents results of different processing of 3D synchronous spectra of the same group of patients with POAG and control group (CTRL) as in the previously published paper [ 12 ]. The results were promising, so we have continued with mathematical processing of published 3D synchronous spectra data [ 12 ] to increase the resolution between the groups (POAG and CTRL). The results published in this paper differ from those previously published [ 12 ] in data processing.…”

Section: Resultsmentioning

confidence: 99%

“…In this article, synchronous excitation spectra of tear fluid from our database were used. The same 3D synchronous spectra of tear fluid of the patients and healthy subjects were used in our previously published pilot study [ 12 ] in which the tear fluid was collected by glass microcapillary at the Department of Ophthalmology, University Hospital Louis Pasteur in Košice from random patients ( Table 1 ) with primary open-angle glaucoma (POAG, n = 13) and healthy subjects (CTRL, n = 8). The patients were treated according to the Declaration of Helsinki.…”

Section: Methodsmentioning

confidence: 99%

“…Description of patients, procedures, and measurement conditions are described in detail in paper [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…The use of 3D-SFS combined with arithmetic processing of the spectra such as second derivative (2D), enhances minor spectral features and allows more reliable identification of changes in a multifluorescent complex mixture [ 11 ]. Our previously published results [ 12 ] show a positive outcome when applying the second derivative to the selected synchronous spectrum Δλ = 110 nm in distinguishing between CTRL and POAG. In this work, the same 3D synchronous spectra of patients and controls were used to implement the idea of using second derivative spectra to identify glaucoma from tear fluid.…”

Section: Introductionmentioning

confidence: 97%

“…A novel strategy has been proposed to evaluate the 3D-SFS. The work builds on a previously published pilot study [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Derivative Three-Dimensional Synchronous Fluorescence Analysis of Tear Fluid and Their Processing for the Diagnosis of Glaucoma

Dubayová

Krajčíková

Mareková

et al. 2022

Sensors

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Background: Sensitive and rapid diagnosis of the early stages of glaucoma from tear fluid is a great challenge for researchers. Methods: Tear fluid was analyzed using three-dimensional synchronous fluorescence spectroscopy (3D-SFS). Our previously published results briefly describe the main methods which applied the second derivative to a selected synchronous spectrum Δλ = 110 nm in distinguishing between healthy subjects (CTRL) and patients with glaucoma (POAG). Results: In this paper, a novel strategy was used to evaluate three-dimensional spectra from the tear fluid database of our patients. A series of synchronous excitation spectra were processed as a front view and presented as a single curve showcasing the overall fluorescence profile of the tear fluid. The second derivative spectrum provides two parameters that can enhance the distinction between CTRL and POAG tear fluid. Conclusions: Combining different types of 3D-SFS data can offer interesting and useful diagnostic tools and it can be used as input for machine learning and process automation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Description of patients, procedures, and measurement conditions are described in detail in paper [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

“…A novel strategy has been proposed to evaluate the 3D-SFS. The work builds on a previously published pilot study [ 12 ].…”

Section: Introductionmentioning