New Role for a Commercially Available Bioinsecticide: Bacillus thuringiensis Berliner Biodegrades the Pyrethroid Cypermethrin

Abstract

The microbial diversity of several environments has been explored by researchers for the biodegradation of pyrethroids. In this study, a new approach was employed aiming at the use of Bacillus thuringiensis Berliner, a strain commercially available as bioinsecticide, for Cypermethrin (Cyp) biodegradation. This bacterial strain grew in the presence of Cyp and biodegraded this xenobiotic in a liquid medium. A central composite design for surface response methodology was employed for biodegradation. Under optimized conditions (50 mg•L −1 of Cyp, pH 8.5, 37 °C), 83.5% biodegradation was determined with the production of 12.0 ± 0.6 mg•L −1 3-phenoxybenzoic acid after 5 days. Moreover, a biodegradation pathway with the 18 compounds identified by GC-MS and LC-MS/MS was proposed. Experiments in soil for 28 days at 30 °C were performed, and 16.7% Cyp degradation was determined under abiotic conditions, whereas 36.6 ± 1.9% biodegradation was observed for B. thuringiensis Berliner with the native microbiome, indicating that bioaugmentation with this strain promoted a significant increase in the Cyp decontamination. Therefore, B. thuringiensis Berliner can act as biodegrader agent and insecticide at the same time, promoting decontamination of chemicals as Cyp while maintaining the protection of crops against insects. Moreover, B. thuringiensis species can produce bacteriocins with antifungal activity, which may increase agricultural productivity.

Expression, Characterization, Fermentation, Immobilization, and Application of a Novel Esterase Est804 From Metagenomic Library in Pesticide Degradation

“…However, the results of our research cannot be directly compared with those of the recent studies on the capacity of PYR degradation by other isolated strains, since the experiments were carried out under the abiotic conditions over a short period of time, but it provides favorable support for developing enzyme preparations that can be industrially produced. According to the study reported by Birolli et al (2021) , only 16.7% CYP degradation was determined under abiotic conditions, whereas 36.6 ± 1.9% biodegradation was observed for B. thuringiensis Berliner with the native microbiome. The example evidently indicated that bioaugmentation with the bacterial strain promoted a significant increase in the pesticide decontamination, prompting that Est804 can be used in biological conditions to enhance the degradation of pyrethroids, which is possibly of great benefit for application in agricultural residue treatment and environmental remediation.…”

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

“…In it, compounds G and K have not been reported in the biodegradation process of fenvalerate. However, 3-aminobenzoic acid has been detected in the biodegradation products of beta-cypermethrin [ 46 ]. Moreover, 3,4,5-trihydroxybenozic acid was detected in the products obtained from the degradation of 3-PBA by strain M-4 [ 47 ].…”

Characteristics and Substrate Specificity of Semi-Purified Bacterial Protease ofBacillusthuringiensisHSFI-12 with Potential as Antithrombotic Agent

“…Refreshment of bacterial isolates was carried out by growing a loopful of HSFI-12 bacterial colony on Nutrien Agar (NA) growth media. The media was incubated in an incubator (Memmert, Germany), at 37ºC for 24 h (Birolli et al, 2021) . The growing colonies were then observed under an optical microscope (Olympus, Japan) for their uniform cell morphology (Purwaningrum et al, 2021) .…”