2013
DOI: 10.1016/j.theriogenology.2013.01.014
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New seminal plasma removal method for freezing stallion semen

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Cited by 15 publications
(13 citation statements)
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“…The spermatozoa retained on the filter were resuspended in Botu‐Bov ® diluent at the same concentration as the control group, according to the technique described by Ramires Neto et al. ().…”
Section: Methodsmentioning
confidence: 99%
“…The spermatozoa retained on the filter were resuspended in Botu‐Bov ® diluent at the same concentration as the control group, according to the technique described by Ramires Neto et al. ().…”
Section: Methodsmentioning
confidence: 99%
“…En concordancia con los resultados encontrados en este trabajo, diferentes investigadores reportan que la centrifugación, genera daños mecánicos sobre los espermatozoides equinos, los cuales dependen de la duración y la fuerza con que sea ejecutada [6]. Se observó que incluso la menor fuerza de centrifugación evaluada (600 x g), produjo una reducción en la integridad y funcionalidad espermática.…”
Section: Resultsunclassified
“…La centrifugación es un proceso utilizado durante el tratamiento del semen, para su almacenamiento en refrigeración y congelación [3,4]. De igual forma, se utiliza dada la necesidad de aumentar la concentración de los espermatozoides, para la inseminación artificial de las yeguas [5,6].…”
Section: El Objetivo De Este Estudio Fue Evaluar El Efecto De La Centunclassified
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“…The percentages of sperm deformities, including head, neck and tail deformities, were investigated at 400× or 1,000× magnification with Giemsa stain. Furthermore, the ejaculate was filtered through a sperm filter to remove seminal plasma and impurities (Ramires Neto et al, ). Semen aliquots were then diluted with Tris‐citric acid extender containing 1.56% citric acid (Fisher Scientific), 3.0% tris‐(hydroxymethyl)‐aminomethane (Research Organics), 20% fresh egg yolk, 0.2% fructose (Scharlau), 6.0% glycerol (Merck), penicillin (1,000 IU/ml) and streptomycin (100 mg/ml) (Akhter et al, ; Andrabi, ).…”
Section: Methodsmentioning
confidence: 99%