New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)

Tahzima, Rachid; Foucart, Yoika; Peusens, Gertie; Beliën, Tim; Massart, Sébastien; Jonghe, Kris De

doi:10.1016/j.jviromet.2018.12.019

Cited by 26 publications

(15 citation statements)

References 57 publications

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“…These results were comparable with those of LAMP testing on other RNA viruses. For examples, LAMP was 100 times more sensitive than rt-PCR on the detection of Citrus leaf blotch virus [21] and Little cherry virus 1 [22]; on Potato virus X [23] and Sugarcane streak mosaic virus [24], LAMP was 10 times more sensitive than rt-PCR. Compared to rt-qPCR, LAMP was similarly sensitive for detection of Onion yellow dwarf virus [25] and Potato leafroll virus [26].…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Sarkes

Feindel

et al. 2020

Preprint

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Tomato brown rugose fruit virus (ToBRFV) is a member of Tobamovirus infecting tomato and pepper. Within North America, both the United States and Mexico consider ToBRFV to be a regulated pest. In Canada, the presence of ToBRFV has been reported, but an efficient diagnostic system has not yet been established. Here, we describe the development and assessment of a loop-mediated isothermal amplification (LAMP)-based assay to detect ToBRFV. The LAMP test was efficient and robust, and results could be obtained within 35 min with an available RNA sample. Amplification was possible when either water bath or oven were used to maintain the temperature at isothermal conditions (65°C), and results could be read by visual observation of colour change. Detection limit of the LAMP was eight target RNA molecules. Under the experimental conditions tested, LAMP was as sensitive as qPCR and 100 times more sensitive than the currently used rt-PCR. We recommend this sensitive, efficient LAMP protocol to be used for routine lab testing of ToBRFV.

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Section: Discussionmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Sarkes

Feindel

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In previous studies, LAMP was 100 times more sensitive than RT-PCR on the detection of Citrus leaf blotch virus [23] and Little cherry virus 1 [24], while on Potato virus X [25] and Sugarcane streak mosaic virus [26], LAMP was 10 times more sensitive than RT-PCR. Compared to RT-qPCR, LAMP was similarly sensitive for detection of Onion yellow dwarf virus [27] and Potato leafroll virus [28].…”

Section: Sensitivity Of the Lampmentioning

confidence: 94%

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV)

Sarkes

Feindel

et al. 2020

PLoS ONE

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Tomato brown rugose fruit virus (ToBRFV) is a member of Tobamovirus infecting tomato and pepper. Within North America, both the United States and Mexico consider ToBRFV to be a regulated pest. In Canada, the presence of ToBRFV has been reported, but an efficient diagnostic system has not yet been established. Here, we describe the development and assessment of a loop-mediated isothermal amplification (LAMP)-based assay to detect ToBRFV. The LAMP test was efficient and robust, and results could be obtained within 35 min with an available RNA sample. Amplification was possible when either water bath or oven were used to maintain the temperature at isothermal conditions (65˚C), and results could be read by visual observation of colour change. Detection limit of the LAMP was eight target RNA molecules. Under the experimental conditions tested, LAMP was as sensitive as qPCR and 100 times more sensitive than the currently used RT-PCR. We recommend this sensitive, efficient LAMP protocol to be used for routine lab testing of ToBRFV.

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“…However, ELSA, molecular hybridization and immunoelectron microscopy have complex experimental procedures and low sensitivity. At present, a diagnostic protocol of LChV-1 based on a onestep RT-LAMP was established, but the experimental results showed that the dependence on a mobile detection device [7]. This study aims to establish a speci c, fast detection method of LChV-1, and lies the foundation for the application of LChV-1 fast diagnosis in the eld.…”

Section: Main Textmentioning

confidence: 99%

Optimization and Application of Loop-mediated Isothermal Amplification Detection Method on Little Cherry Virus 1

De-ya¹,

Li²,

Yang³

et al. 2021

Preprint

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Background: Little cherry virus 1 (LChV-1) is one of the most pathogenic virus that frequently infect cherry and have caused serious economic damage to production and quality of cherry. But current detection methods of LChV-1 was less.Objective: This study aims to optimization of loop-mediated isothermal amplification (LAMP) detection method on Little cherry virus 1, and lies the foundation for the application of LChV-1 fast diagnosis in the field in Shandong province.Methods: We performed one-step RT-LAMP at temperature of 59℃ for 60 minutes, whereas the reaction system contained 5.2 mM Mg2+, 8 U Bst 2.0 DNA polymerase, 1.4 mM deoxyribonucleotide and 0.2 and 1.6 µM of the outer and inner primers, respectively. And,we used the RT-LAMP method to detect 65 field samples from Shandong province, and discovered that 5 samples were infected by LChV-1, the detection results were consistent with the RT-PCR results.Conclusions: The RT-LAMP method is highly specific, sensitive, practicable and efficient and suitable method for detection LChV-1 samples.

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New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)

Cited by 26 publications

References 57 publications

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection ofTomato brown rugose fruit virus(ToBRFV)

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV)

Optimization and Application of Loop-mediated Isothermal Amplification Detection Method on Little Cherry Virus 1

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