New Technique for Assessing Acrosomal Characteristics of Spermatozoa

Wells, M. E.; Awa, O.A.

doi:10.3168/jds.s0022-0302(70)86184-7

Cited by 201 publications

(99 citation statements)

References 10 publications

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“…m~y be caused by nutrition, environmental temperature, ejaculation frequency, and the normal aging process (Awa, 1970 andSaacke, 1970). Because of the acrosomes labile nature, Awa (1970) and Wells and Awa (1970) Estimates of sperm production of animals has always been used to evaluate the spermatogenic function of the testis. It is of particu~ar interest to A. I. organizations which can turn production into an economic advantage.…”

Section: Sperm Characteristicsmentioning

confidence: 99%

Evaluation of Epididymal Sperm by the Cannulation Technique and the Effects of In Vivo Storage in Angus Bulls

Deutscher

Wells

Battaglia

1974

Journal of Animal Science

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Section: Sperm Characteristicsmentioning

confidence: 99%

Evaluation of Epididymal Sperm by the Cannulation Technique and the Effects of In Vivo Storage in Angus Bulls

Deutscher

Wells

Battaglia

1974

Journal of Animal Science

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“…One drop of evenly mixed sample was supplied to hemocytometer using the improved Neubauer's (Deep 1/110mm.LABART ,Germany) counting Chamber as described by Pant and Srivastava(2003). Live /dead ratio was determined by using 1% Eosin and 5% Nitrosin in 3% sodium nitrate dehydrate solution according to the method described by Wells and Awa (1970).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Effects of Fasting On Fertility in Adult Male Wistar Rats

B.O¹

2012

IOSJPBS

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“…The occurrence of capacitation was determined by the ability to undergo acrosome reaction (AR) in the presence of calcium ionophore A23187 (Tardif et al, 1999). Acrosomal reaction was visualised by Wells-Awa staining (Wells and Awa, 1970) and the viability was analysed by eosin-nigrosin staining method at the end of the incubation period.…”

Section: Sperm Capacitationmentioning

confidence: 99%

Heparin binding analysis of boar sperm and its relation with farrowing capacity

Dapino

Teijeiro

Cane³

et al. 2014

Acta Veterinaria Hungarica

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New methods for the evaluation of semen quality according to in vivo reproductive data are useful tools for identifying boars of lower fertility among individuals with standard semen parameters. In this study, indirect fluorescence microscopy was used to evaluate the heparin binding site distribution upon capacitation of sperm from eight boars arbitrarily distributed into Groups I and II according to differential farrowing rates (Group I: ≥ 70%, Group II: < 70%). Additionally, the ability of sperm to bind to solubilised zona pellucida (ZP) was assayed in the presence or absence of heparin. Samples of two individuals of Group II showed lower percentage of B pattern in relation to other individuals (P < 0.001). The number of spermatozoa attached to ZP after 2 h of incubation in capacitating conditions with heparin was significantly lower than in its absence (P < 0.0001). These results suggest that heparin binding site distribution concerning capacitation may be indicative of the availability of proteins involved in the fertilisation process, specifically at the initial sperm-oocyte recognition. Differences in heparin binding site dynamics during capacitation may help identify a subpopulation of individuals with lower fertilising capacity and normal spermiogram, which is particularly useful at high-production establishments.

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New Technique for Assessing Acrosomal Characteristics of Spermatozoa

Cited by 201 publications

References 10 publications

Evaluation of Epididymal Sperm by the Cannulation Technique and the Effects of In Vivo Storage in Angus Bulls

Evaluation of Epididymal Sperm by the Cannulation Technique and the Effects of In Vivo Storage in Angus Bulls

Evaluation of the Effects of Fasting On Fertility in Adult Male Wistar Rats

Heparin binding analysis of boar sperm and its relation with farrowing capacity

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