New traits in crops produced by genome editing techniques based on deletions

Wiel, C.C.M. van de; Schaart, Jan G.; Lotz, L.A.P.; Smulders, M.J.M.

doi:10.1007/s11816-017-0425-z

Cited by 66 publications

(41 citation statements)

References 76 publications

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“…Furthermore, gene editing has shown great promise in creating new allelic variants, using various technologies such zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) [145][146][147][148]. Gene editing can result in gene modification (e.g.…”

Section: Modern Approaches Technologies and Tools To Benefit Choicementioning

confidence: 99%

Past, Present and Future Perspectives on Groundnut Breeding in Burkina Faso

Konaté¹,

Sanou²,

Miningou³

et al. 2019

Preprint

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Groundnut (Arachis hypogaea L.) is a major food and cash crop in Burkina Faso. Due to growing demand for raw oilseeds, there is an increasing interest in groundnut production from traditional rain-fed areas to irrigated environments. However, despite implementation of many initiatives in the past to increase groundnut productivity and production, the groundnut industry still struggles to prosper, due to several constraints including minimal development research and fluctuating markets. Yield penalty due to drought and biotic stresses continue to be a major drawback for groundnut production. This review traces progress in the groundnut breeding that started in Burkina Faso before the country’s political independence in 1960 through to present times. Up to the 1980s, groundnut improvement was led by international research institutions such as IRHO (Institute of Oils and Oleaginous Research) and ICRISAT (International Crops Research Institute for the Semi-Arid Tropics). However, international breeding initiatives were not sufficient to establish a robust domestic groundnut breeding programme. This review also provides essential information about opportunities and challenges of groundnut research in Burkina Faso, emphasising the need for institutional attention to genetic improvement of the crop.

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Section: Modern Approaches Technologies and Tools To Benefit Choicementioning

confidence: 99%

Past, Present and Future Perspectives on Groundnut Breeding in Burkina Faso

Konaté¹,

Sanou²,

Miningou³

et al. 2019

Preprint

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“…However, gene editing approaches are still in their infancy, and further improvements are necessary. For instance, despite its high specificity, the CRISPR-Cas9 method may still have potential off-target effects, which could be reduced by increasing the specificity of Cas9, the RNA-guided DNA endonuclease protein, and the guide RNA design, or using alternative endonucleases with higher specificity [147]. One of the steps in gene editing that also needs to be refined is the delivery of the new genetic material.…”

Section: Efforts To Control Pod Shatteringmentioning

confidence: 99%

“…However, the same methods can also be used to silence endogenous genes. This is especially important in crop improvement, because many advantageous traits, such as loss of shattering, are often caused by recessive loss-of-function mutations [147]. So far, most of these recent gene editing techniques have focused on disease resistance in crops, but same techniques can be used to improve other traits including shattering-resistance, reduced allergenicity, abiotic stress tolerance, and increased nutritional value [141].…”

Section: Efforts To Control Pod Shatteringmentioning

confidence: 99%

Pod Shattering: A Homologous Series of Variation Underlying Domestication and an Avenue for Crop Improvement

Ogutcen¹,

Pandey²,

Khan³

et al. 2018

Preprint

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In wild habitats, fruit dehiscence is a critical strategy for seed dispersal; however, in cultivated crops it is one of the major sources of yield loss. Therefore, indehiscence of fruits, pods, etc., was likely to be one of the first traits strongly selected in crop domestication. Even with the historical selection against dehiscence in early domesticates, it is a trait still targeted in many breeding programs, particularly in minor or underutilized crops. Here, we review of this trait in pulse (grain legume) crops, which are of growing importance as a source of protein in human and livestock diets, and which have received less attention iii) the molecular pathways underlying this important trait, iv) an overview of the extent of crop losses due to shattering, and the effects of environmental factors on shattering, and, v) efforts to reduce shattering in crops. While our focus is mainly pulse crops, we also included comparisons to crucifers and cereals because there is extensive research on shattering in these taxa.

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“…Site-directed nucleases induce double-strand breaks (DSBs) in the DNA which are subsequently repaired by the hosts own cellular mechanisms. The type of repair can be categorized in three main types [11][12][13][14]:…”

Section: Glossary: [2 5]mentioning

confidence: 99%

What is the available evidence for the application of genome editing as a new tool for plant trait modification and the potential occurrence of associated off-target effects: a systematic map protocol

et al. 2018

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Background: Plant breeding is a developing process and breeding methods have continuously evolved over time. In recent years, genome editing techniques such as clustered regularly interspaced short palindromic repeats/CRISPR associated proteins (CRISPR/Cas), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFN), meganucleases (MN) and oligonucleotide-directed mutagenesis (ODM) enabled a precise modification of DNA sequences in plants. Genome editing has already been applied in a wide range of plant species due to its simplicity, time saving and cost-effective application compared to earlier breeding techniques including classical mutagenesis. Although genome editing techniques induce much less unintended modifications in the genome (off-target effects) compared to classical mutagenesis techniques, off-target effects are a prominent point of criticism as they might cause genomic instability, cytotoxicity and cell death. Methods: The aim of this systematic map is to address the following primary question: "What is the available evidence for the application of genome editing as a new tool for plant trait modification and the potential occurrence of associated off-target effects"? The primary question will be considered by two secondary questions: One is aimed at the traits being modified by genome editing in plants and the other explores the occurrence of off-target effects. The systematic map will focus on model plants as well as on plants produced for agricultural production that were subjected to genome editing techniques. Academic and grey literature will be searched in English and German language. Inclusion/exclusion criteria were developed for the two secondary questions and will be applied on title/abstract and full text stage. Included studies will be catalogued in a searchable and open access database and study results will be summarized using descriptive statistics. Furthermore, the extracted data will serve as a preparatory step for further in-depth analysis, e.g. by a systematic review.

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New traits in crops produced by genome editing techniques based on deletions

Cited by 66 publications

References 76 publications

Past, Present and Future Perspectives on Groundnut Breeding in Burkina Faso

Past, Present and Future Perspectives on Groundnut Breeding in Burkina Faso

Pod Shattering: A Homologous Series of Variation Underlying Domestication and an Avenue for Crop Improvement

What is the available evidence for the application of genome editing as a new tool for plant trait modification and the potential occurrence of associated off-target effects: a systematic map protocol

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