New Transformation Technologies for Trees

Alburquerque, Nuria; Baldacci‐Cresp, Fabien; Baucher, Marie; Casacuberta, Josep; Collonnier, Cécile; Jaziri, Mondher El; Nogué, Fabien; Burgos, L.

doi:10.1007/978-94-017-7531-1_3

Cited by 5 publications

(5 citation statements)

References 176 publications

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“…Traits targeted include wood properties (lignin content and composition), early/late flowering, male sterility to address biosafety regulation issues, enhanced yield traits and herbicide tolerance. These extensive transgenic experiments have shown differences in recalcitrance to in vitro regeneration and genetic transformation in some of the most important commercial hybrid poplars (Alburquerque et al, ). Further, transgene expression stability is being studied.…”

Section: Poplarmentioning

confidence: 99%

Breeding progress and preparedness for mass‐scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow and poplar

et al. 2018

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Genetic improvement through breeding is one of the key approaches to increasing biomass supply. This paper documents the breeding progress to date for four perennial biomass crops (PBCs) that have high output–input energy ratios: namely Panicum virgatum (switchgrass), species of the genera Miscanthus (miscanthus), Salix (willow) and Populus (poplar). For each crop, we report on the size of germplasm collections, the efforts to date to phenotype and genotype, the diversity available for breeding and on the scale of breeding work as indicated by number of attempted crosses. We also report on the development of faster and more precise breeding using molecular breeding techniques. Poplar is the model tree for genetic studies and is furthest ahead in terms of biological knowledge and genetic resources. Linkage maps, transgenesis and genome editing methods are now being used in commercially focused poplar breeding. These are in development in switchgrass, miscanthus and willow generating large genetic and phenotypic data sets requiring concomitant efforts in informatics to create summaries that can be accessed and used by practical breeders. Cultivars of switchgrass and miscanthus can be seed‐based synthetic populations, semihybrids or clones. Willow and poplar cultivars are commercially deployed as clones. At local and regional level, the most advanced cultivars in each crop are at technology readiness levels which could be scaled to planting rates of thousands of hectares per year in about 5 years with existing commercial developers. Investment in further development of better cultivars is subject to current market failure and the long breeding cycles. We conclude that sustained public investment in breeding plays a key role in delivering future mass‐scale deployment of PBCs.

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Section: Poplarmentioning

confidence: 99%

Breeding progress and preparedness for mass‐scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow and poplar

et al. 2018

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“…Despite their advantages, transgenesis has its own limitations, including the random integration of transgenes into the genome and the fact that many fruit trees species are recalcitrant or time-consuming in their transformation. Therefore, it is paramount to enhance transgenic research and dedicate additional efforts to enhance the efficiency of the regeneration and transformation procedures in fruit trees [5]. Equally, in higher plants, achieving the insertion of DNA sequences at a precise genomic location using homologous recombination, known as gene targeting (GT), has remained challenging due to the notably low efficiency of homologous recombination [6].…”

Section: Of 23mentioning

confidence: 99%

“…Among the new technologies developed in recent years, various site-specific nucleases (SSNs) have emerged, enabling the precise creation of double-strand breaks (DSBs) at specific locations within the genome. These SSNs offer a highly innovative approach to genome engineering, facilitating targeted modifications such as gene silencing, gene correction, and gene addition [5]. SSNs have significant economic, time-saving, and streamlined advantages relative to conventional breeding methods, which may take up to approximately a decade in order to develop a variety [8].…”

Section: Of 23mentioning

confidence: 99%

“…The classification of SSN-based genome-editing systems is according to the following categories: meganucleases, zinc finger nucleases (ZFNs), transcription-like effector nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), which is associated with the RNA-guided Cas double-stranded DNA-binding protein (CRISPR/Cas system) [5,10]. The main differences among them are their relative specificity and efficiency [11].…”

Section: Of 23mentioning

confidence: 99%

“…However, there are several limitations in the transformation of fruit trees. Most of fruit trees are recalcitrant to regeneration and/or transformation, the processes are genotype-dependent, the process is time-consuming compared to for other species, and accurate selection with antibiotics or herbicides is necessary to avoid chimeric plants [5]. Furthermore, the lack of available and efficient explants for regeneration and transformation procedures (e.g., seedlings, leaves from micropropagated plants, or immature seeds) makes difficult the establishment of effective protocols [28].…”

Section: Genetic Transformation Technology In Fruit Treesmentioning

confidence: 99%

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CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

Martín-Valmaseda,

Devin,

Ortuño-Hernández

et al. 2023

IJMS

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CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria. In this work, we reviewed the methods used to introduce CRISPR/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out target genes in different fruit tree species, including on tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses. The application of this gene-editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits into elite cultivars without changing other traits. However, currently, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are genotype-dependent, and the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, the latest news on the legislation and regulations about the use of plants modified using CRISPR/Cas systems has been also discussed.

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Cis Genesis of Crops

Hefferon

2022

Concepts and Strategies in Plant Sciences

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New Transformation Technologies for Trees

Cited by 5 publications

References 176 publications

Breeding progress and preparedness for mass‐scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow and poplar

Breeding progress and preparedness for mass‐scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow and poplar

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

Cis Genesis of Crops

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