2013
DOI: 10.1128/aem.01065-13
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New Type of Antimicrobial Protein Produced by the Plant Pathogen Clavibacter michiganensis subsp. michiganensis

Abstract: It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I… Show more

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Cited by 14 publications
(10 citation statements)
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“…The aureocin A53 concentration required for 50% growth inhibition (IC 50 ) of M. luteus ATCC 4698 was determined as described by Liu et al . (). The therapeutic index for aureocin A53 was determined as the CC 50 for each cell line divided by the IC 50 .…”
Section: Methodsmentioning
confidence: 97%
See 1 more Smart Citation
“…The aureocin A53 concentration required for 50% growth inhibition (IC 50 ) of M. luteus ATCC 4698 was determined as described by Liu et al . (). The therapeutic index for aureocin A53 was determined as the CC 50 for each cell line divided by the IC 50 .…”
Section: Methodsmentioning
confidence: 97%
“…Changes in cell morphology were also investigated by using a Leitz inverted microscope (model 633456; E. Leitz Inc, Rockleigh, NJ). The aureocin A53 concentration required for 50% growth inhibition (IC 50 ) of M. luteus ATCC 4698 was determined as described by Liu et al (2013). The therapeutic index for aureocin A53 was determined as the CC 50 for each cell line divided by the IC 50 .…”
Section: Cytotoxic Effectsmentioning
confidence: 99%
“…In order to improve the efficiency of protein purification, Fusion protein tags are used for bacteriocins heterologous expression (Liu et al., 2013; Moon et al., 2006; Nigutova et al., 2008; Parret, Wyns, De Mot, & Loris, 2004; Yang et al., 2007; Yu et al., 2020). Fusion protein with the Tags (His, GST, Intein, and SUMO) was used in the purification of different kinds of bacteriocins (see Table 5).…”
Section: Purification and Identification Of Bacteriocinsmentioning
confidence: 99%
“…The mechanism of their action was the attachment of magainin molecules to microorganisms and destroying of their cell membranes [5]. Membrane permeability is the main mechanism to describe the action of antimicrobial peptides [6][7][8]. Most antibacterial peptides are positively charged and have an amphiphilic character.…”
Section: Introductionmentioning
confidence: 99%
“…These cationic AMPs can interact with the negatively charged bacterial cell membranes, causing changes to the electrochemical potential and the permeation of molecules. This affects the destruction of cell membranes and ultimately causes cell death [6][7][8][9]. The therapeutic use of AMP is that they are selective for the type of cell membrane.…”
Section: Introductionmentioning
confidence: 99%