New Ulcerative Colitis Model Induced by Sulfhydryl Blockers in Rats and the Effects of Antiinflammatory Drugs on the Colitis

Abstract

-Phenylephrine displacement curves for the specific binding of [3H]prazosin in the mem brane fraction prepared from rabbit thoracic aorta showed high and low-affinity sites with slope factors significantly less than unity. The irreversible a 1B-antagonist phenoxybenzamine shifted the binding sites to single high affinity sites with a slope factor close to unity in the presence of the metabolically stable GTP analog GTP y-S. These results indicate that phenoxybenzamine may have affected selectively the low affinity site to phenylephrine in the presence of GTP y-S.

Acute and chronic NOS inhibition enhances α2- adrenoreceptor-stimulated RhoA and Rho kinase in rat aorta

“…The potential role of RhoA and ROK in ␣ 2 -AR contraction is suggested by studies in other systems. For example, norepinephrine contraction in human small omental arteries and rabbit aorta is sensitive to inhibition by Y-27632 (27) and C3 botulinum toxin (24), respectively, suggesting that RhoA and ROK contribute to adrenergic arterial contraction. Carbachol-induced actin formation in human airway smooth muscle requires G i activation of RhoA (39).…”

An evaluation of animal models for using bioactive compounds in the treatment of inflammatory bowel disease

“…Iodoacetamide is a hydrophobic compound blocker that can reduce the level of endogenous sulfhydryl compounds such as glutathione, which has a protective effect on the gastric mucosa (Goyal et al., 2014; Satoh et al., 1997). It can also denature proteins that contain disulfide bonds in cells, leading to the distortion of the normal structure and function of cells.…”