New wound dressing polymeric nanofiber containing green tea extract prepared by electrospinning method

Abstract

The effect of green tea extract as a natural and ecofriendly antibacterial additive on the healing effect of chitosan/ polyethylene oxide wound dressing nanofiber was investigated. Three different polymeric nanofiber composite including chitosan, chitosan/polyethylene oxide and chitosan/polyethylene oxide/green tea extract, was produced by electrospinning method and was used as wound dressing. Affecting parameters on electrospinning method such as voltage, feed rate, nozzlecollector distance and content of green tea in nanofiber structure were studied and optimized. The morphology of electrospun nanofibers was studied with use of scanning electron microscope. The average diameters of prepared nanofibers was determined by Image J software. The optimum conditions for preparation of electrospun nanofibers were: Voltage; 20 kV, feed rate; 0.5 ml/h, nozzle-collector distance; 10 cm, and chitosan/polyethylene oxide weight ratio; 0.9. Stability, water absorption and degradation of prepared nanofibers were investigated by placing the prepared nanofibers for 24 hours in a solution of acetate buffer with a pH of 5.5 and a temperature of 37 o C. Moreover, the antibacterial activity of composite scaffolds were tested by Agar plate method by two type bacteria including Escherichia coli and Staphylococcus aureus. Furthermore, the healing ability of the prepared nanofibers was studied on the rat's wound. Chitosan/polyethylene oxide/ green tea showed the best healing effects in comparison with the other prepared wound dressings. These results confirmed that green tea extract helps to keep wound surface moist, reduces inflammation and increases the speed of recovery and healing.

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

“…Moreover, in recent years the combination of synthetic biomaterials and biological molecules based on plant-derived compounds has started to be used widely for wound healing approaches (Ramos-e- Silva and Ribeiro de Castro, 2002;Dhivya et al, 2015). Although the use of phenols and essential oils (EOs) as natural active agents is rather novel in combination with engineered biomaterials for biomedical applications (Sadri et al, 2015;Li et al, 2018;Jaganathan et al, 2019), there is increasing interest for this kind of substances in medicine, considering the fact that EOs have been used by humans for thousands of years to obtain therapeutic effects (Silva and Fernandes Júnior, 2010).…”

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

“…Several researchers have investigated different methods to fabricate biomimetic skin scaffold. [ 16,17,19,20 ] The first commonly used approach is the electrospinning technique that has been intensively applied due to the advantages of reproducibility, simplicity, and diversity in producing fibers with micro‐ or nanoscale diameters and different topographical features. [ 21,22 ] The fibers' membrane produced by electrospinning can provide large surface areas for cells to attach and proliferate, which could also enhance the penetration of nutrients and oxygen.…”

Drug loading and delivery using nanofibers scaffolds

“…Some important applications of nanofibers in transdermal systems and wound dressings are discussed in Table 1. (Jung et al 2015) Poly(ethylene oxide) (PEO)-polycaprolactone blended and silver nanoparticle (Ag NP) incorporated composite nanofiber scaffolds -antibacterial wound dressings (Dubey et al 2015) Three different polymeric nanofiber composite including chitosan, chitosan/ polyethylene oxide, and chitosan/ polyethylene oxide Green tea extract Green tea extract helps to keep wound surface moist, reduces inflammation, and increases the speed of recovery and healing (Sadri et al 2015) Silver sulfadiazine Silk Epidermal growth factor Re-epithelialization, dermis proliferation, collagen synthesis (Gil et al 2013) Polycaprolactone ( …”