Newcastle Disease Virus Replication

Peeples, Mark E.

doi:10.1007/978-1-4613-1759-3_4

Cited by 19 publications

(16 citation statements)

References 126 publications

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“…The genomic RNA is 15,186 nucleotides in length (21,34). The genomic RNA contains six genes that encode at least seven proteins (33,44). The envelope of NDV contains two glycoproteins, the hemagglutinin-neuraminidase (HN) and fusion (F) proteins.…”

Section: Newcastle Disease Virus (Ndv) Is a Member Of The Familymentioning

confidence: 99%

Loss of N-Linked Glycosylation from the Hemagglutinin- Neuraminidase Protein Alters Virulence of Newcastle Disease Virus

Panda

Elankumaran

Krishnamurthy

et al. 2004

J Virol

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The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) is an important determinant of its virulence. We investigated the role of each of the four functional N-linked glycosylation sites (G1 to G4) of the HN glycoprotein of NDV on its pathogenicity. The N-linked glycosylation sites G1 to G4 at residues 119, 341, 433, and 481, respectively, of a moderately pathogenic NDV strain Beaudette C (BC) were eliminated individually by site-directed mutagenesis on a full-length cDNA clone of BC. A double mutant (G12) was also created by eliminating the first and second glycosylation sites at residues 119 and 341, respectively. Infectious virus was recovered from each of the cDNA clones of the HN glycoprotein mutants, employing a reverse genetics technique. There was a greater delay in the replication of G4 and G12 mutant viruses than in the parental virus. Loss of glycosylation does not affect the receptor recognition by HN glycoprotein of NDV. The neuraminidase activity of G4 and G12 mutant viruses and the fusogenicity of the G4 mutant virus were significantly lower than those of the parental virus. The fusogenicity of the double mutant virus (G12) was significantly higher than that of the parental virus. Cell surface expression of the G4 virus HN was significantly lower than that of the parental virus. The antigenic reactivities of the mutants to a panel of monoclonal antibodies against the HN protein indicated that removal of glycosylation from the HN protein increased (G1, G3, and G12) or decreased (G2 and G4) the formation of antigenic sites, depending on their location. In standard tests to assess virulence in chickens, all of the glycosylation mutants were less virulent than the parental BC virus, but the G4 and G12 mutants were the least virulent.

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Section: Newcastle Disease Virus (Ndv) Is a Member Of The Familymentioning

confidence: 99%

Loss of N-Linked Glycosylation from the Hemagglutinin- Neuraminidase Protein Alters Virulence of Newcastle Disease Virus

Panda

Elankumaran

Krishnamurthy

et al. 2004

J Virol

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“…RNA sequencing studies revealed that the 39 and 59 ends of the genomic RNA respectively contain a leader sequence and a trailer sequence of some 50 nucleotides (Kurilla et al, 1985;Yusoff et al, 1987). Transcription of the leader sequence results in the production of the leader transcript, which is the smallest but most abundant mRNA (Peeples, 1988). Recently, Phillips et al (1998) showed that the trailer region of Beaudette C has 114 nucleotides.…”

Section: Viral Genomementioning

confidence: 99%

“…Direct transcription of the start signals (39 -UGCCCAUCU/CU-59 ) preceding the six major genes results in six mRNAs, which are capped, methylated and terminated at a common polyadenylation site (39 -AA/UCUUUUUU-59 ) (Ishada et al, 1986;Millar et al, 1986;Yusoff et al, 1987). Unlike these mRNAs, the leader transcript is not capped, methylated or polyadenylated (Peeples, 1988). Transcriptional modification of the P gene mRNA, by insertion of one or two non-templated G residues at nucleotide position 484 in the P transcript, produces extra species of modified mRNAs (Steward et al, 1993;Locke et al, 2000) that encode non-structural proteins, V (+ one nucleotide frameshift) and W (+ two nucleotide frameshifts), respectively.…”

Section: Viral Genomementioning

confidence: 99%

Newcastle disease virus: Macromolecules and opportunities

Yusoff¹,

Tan²

2001

Avian Pathology

141

111

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Over the past two decades, enormous advances have occurred in the structural and biological characterization of Newcastle disease virus (NDV). As a result, not only the complete sequence of the viral genome has been fully determined, but also a clearer understanding of the viral proteins and their respective roles in the life cycle has been achieved. This article reviews the progress in the molecular biology of NDV with emphasis on the new technologies. It also identifies the fundamental problems that need to be addressed and attempts to predict some research opportunities in NDV that can be realized in the near future for the diagnosis, prevention and treatment of disease(s).

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“…Virions are then formed by budding from the cell membrane, but the roles of the virion components in the initiation and control of the budding process are poorly understood, although the matrix protein is believed to be of major importance in this regard (Peeples, 1988). In our studies the matrix protein was lacking in the recombinant as shown by Western blot analysis with polyclonal antisera to NDV, and by the failure of antisera against extracts of cells infected with the recombinant to react with N D V proteins other than H N (Nagy et al, 1990).…”

Section: ~ Department Of Veterinary Microbiology and Immunology And Zmentioning

confidence: 99%

Synthesis of Newcastle disease virus (NDV)-like envelopes in insect cells infected with a recombinant baculovirus expressing the haemagglutinin-neuraminidase of NDV

Nagy¹,

Huber²,

Krell

et al. 1991

Journal of General Virology

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Newcastle Disease Virus Replication

Cited by 19 publications

References 126 publications

Loss of N-Linked Glycosylation from the Hemagglutinin- Neuraminidase Protein Alters Virulence of Newcastle Disease Virus

Loss of N-Linked Glycosylation from the Hemagglutinin- Neuraminidase Protein Alters Virulence of Newcastle Disease Virus

Newcastle disease virus: Macromolecules and opportunities

Synthesis of Newcastle disease virus (NDV)-like envelopes in insect cells infected with a recombinant baculovirus expressing the haemagglutinin-neuraminidase of NDV

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