Newly Formed Bone in Mandible Decortication Experimental Model Using rhBMP-2 Evaluated by Densitometric Study

Abstract

The purpose of this study was to evaluate the newly formed bone, comparing two different carriers for rhBMP-2, using the decortication and nondecorticatication surgical technique in Wistar rat mandibles, evaluated by radiographic densitometry method. It was used fifty six animals according to specific treatment, which were sacrificed after 3 and 6 weeks after this. It was concluded that the decortication surgical technique was able to optimize the osteoinduction properties of the rhBMP-2, independently of the material carrier used and the period of time, according to radiographic densitometry technique.

Radiodensitometric and DXA analyses for the measurement of bone mineral density after systemic alendronate therapy

“…25 The measurement of BMD by radiographic densitometry has been performed in a number of studies, and its efficacy has been confirmed. 1,4,7,26 Radiographic densitometry may be considered as a minimally invasive and convenient method for diagnosing osteoporosis. 1 According to Erdogan et al, 1 the radiological parameters can facilitate the establishment of a definite diagnosis of osteoporosis.…”

“…18 In addition to radiographic densitometry, the contemporary methods of bone densitometry include single-photon absorptiometry, single-energy x-ray absorptiometry, dual-photon absorptiometry and DXA. 7 The measurement of BMD in long bones or the spine by DXA is currently recognized as a well-established method for the diagnosis of osteoporosis. 10 The operation of DXA devices is based on the principle that the bone and soft tissue have different attenuation properties as a function of photon energy.…”

“…Bone densitometry is commonly used in medicine to measure BMD, 7 and it is an important method for the early diagnosis of osteopenic and osteoporotic conditions, as well as for monitoring treatment evolution. 18 In addition to radiographic densitometry, the contemporary methods of bone densitometry include single-photon absorptiometry, single-energy x-ray absorptiometry, dual-photon absorptiometry and DXA.…”

“…The sodium alendronate (n = 15) and control (n = 10) tibias were arranged symmetrically on extraoral radiographic films (Kodak  Min-R S, Kodak Eastman Co., Rochester, USA) together with an aluminum stepwedge with 8 steps (NDT Mart Inc., Covington, USA), ranging from 2 to 16 mm, which served as a reference for densitometric analysis. Radiographic densitometry was performed based on the studies by Issa et al 7 and Erdogan et al 1 The tube of the x-ray unit (Weber x-ray unit; The Weber Dental Mfg. Co., Canton, USA) was placed perpendicular to the film, and a single radiographic exposure was made with exposure parameters of 10 mA, 60 kV, 0.6 seconds and 40 cm focus-film distance.…”

“…6 Bone densitometry facilitates the early diagnosis of metabolic bone diseases associated with intense bone resorption, such as osteopenic and osteoporotic conditions, and this method can be used to monitor treatment efficacy, thereby significantly decreasing the incidence of pathological fractures. 7 Some non-invasive imaging technologies, such as magnetic resonance imaging (MRI), ultrasound, computed tomography (CT), dual-energy x-ray absorptiometry (DXA) 8 and radiographic densitometry, 1 have been employed in medical diagnosis for different purposes. Furthermore, periapical and panoramic radiography techniques, which are commonly used in dental practice, could also be useful for the detection of bone disorders.…”