Newly identified genes contribute to vanillin tolerance in <i>Saccharomyces cerevisiae</i>

Liang-gang, Zong; Wang, Xinning; Bao, Xinhe; Wei, Tian‐Ran; Hou, Jin; Liu, Weifeng; Shen, Yu

doi:10.1111/1751-7915.13643

Cited by 18 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mbf1 is a transcriptional coactivator that mediates transcriptional activation by bridging the DNA-binding region of an activator and a TBP (TATA-binding protein) [ 55 ]. However, recently, Liang et al have confirmed that the TBP binding function of Mbf1 was not the reason for the enhancement of vanillin resistance mediated by Mbf1p [ 56 ]. Under stress conditions, Tma17 (also named as Adc17) is the most important chaperone for proteasome biogenesis and assembly in yeast cells.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Vanillin is one of the important phenolic inhibitors in Saccharomyces cerevisiae for bioconversion of lignocellulosic materials and has been reported to inhibit the translation process in cells. In our previous studies, it was confirmed that the deletion of the transcription factor gene YRR1 enhanced vanillin resistance by promoting some translation-related processes at the transcription level. In this work, we investigated the effects of proteomic changes upon induction of vanillin stress and deletion of YRR1 to provide unique perspectives from a transcriptome analysis for comprehending the mechanisms of YRR1 deletion in the protective response of yeast to vanillin. Results In wild-type cells, vanillin reduced two dozens of ribosomal proteins contents while upregulated proteins involved in glycolysis, oxidative phosphorylation, and the pentose phosphate pathway in cells. The ratios of NADPH/NADP+ and NADH/NAD+ were increased when cells responded to vanillin stress. The differentially expressed proteins perturbed by YRR1 deletion were much more abundant than and showed no overlaps with transcriptome changes, indicating that Yrr1 affects the synthesis of certain proteins. Forty-eight of 112 upregulated proteins were involved in the stress response, translational and transcriptional regulation. YRR1 deletion increased the expression of HAA1-encoding transcriptional activator, TMA17-encoding proteasome assembly chaperone and MBF1-encoding coactivator at the protein level, as confirmed by ELISA. Cultivation data showed that the overexpression of HAA1 and TMA17 enhanced resistance to vanillin in S. cerevisiae. Conclusions Cells conserve energy by decreasing the content of ribosomal proteins, producing more energy and NAD(P)H for survival in response to vanillin stress. Yrr1 improved vanillin resistance by increasing the protein quantities of Haa1, Tma17 and Mbf1. These results showed the response of S. cerevisiae to vanillin and how YRR1 deletion increases vanillin resistance at the protein level. These findings may advance our knowledge of how YRR1 deletion protects yeast from vanillin stress and offer novel targets for genetic engineering of designing inhibitor-resistant ethanologenic yeast strains.

show abstract

Section: Resultsmentioning

confidence: 99%

Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yeast strain growth capacity on WXML was determined by the spotting assay (Liang et al ., 2021). Overnight cultures were transferred into fresh YPD medium with an initial OD 600 of 0.2 and cultured for another 12 h. The cells were then harvested and washed three times with ddH 2 O and resuspended in ddH 2 O.…”

Section: Methodsmentioning

confidence: 99%

Production of xylitol by Saccharomyces cerevisiae using waste xylose mother liquor and corncob residues

Chen

et al. 2021

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

Exorbitant outputs of waste xylose mother liquor (WXML) and corncob residue from commercial-scale production of xylitol create environmental problems. To reduce the wastes, a Saccharomyces cerevisiae strain tolerant to WXML was conferred with abilities to express the genes of xylose reductase, a xylosespecific transporter and enzymes of the pentose phosphate pathway. This strain showed a high capacity to produce xylitol from xylose in WXML with glucose as a co-substrate. Additionally, a simultaneous saccharification and fermentation (SSF) process was designed to use corncob residues and cellulase instead of directly adding glucose as a co-substrate. Xylitol titer and the productivity were, respectively, 91.0 g l -1 and 1.26 AE 0.01 g l -1 h -1 using 20% WXML, 55 g DCW l -1 delignified corncob residues and 11.8 FPU g cellulose -1 cellulase at 35°during fermentation. This work demonstrates the promising strategy of SSF to exploit waste products to xylitol fermentation process.

show abstract

“…The genes involved in mRNA synthesis that directly affect transcriptional control and RNA process are downregulated in response to furfural, and the genes involved in mitochondrial ribosomal proteins are downregulated in response to acetic acid [ 86 ]. High concentrations of vanillin result in the repression of translational and the formation of cytosolic mRNP granules, leading to a reduction in overall protein synthesis levels and the limited translation of mRNAs [ 68 , 101 – 103 ]. Regulating the expression of these genes to relieve stress-induced transcriptional and translational repression can be beneficial to enhance the tolerance of strain.…”

Section: Response Mechanisms Of S Cerevisiae To St...mentioning

confidence: 99%

Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

Liu

Zhao

2022

Biotechnol Biofuels

View full text Add to dashboard Cite

Bioconversion of lignocellulosic biomass to biofuels such as bioethanol and high value-added products has attracted great interest in recent decades due to the carbon neutral nature of biomass feedstock. However, there are still many key technical difficulties for the industrial application of biomass bioconversion processes. One of the challenges associated with the microorganism Saccharomyces cerevisiae that is usually used for bioethanol production refers to the inhibition of the yeast by various stress factors. These inhibitive effects seriously restrict the growth and fermentation performance of the strains, resulting in reduced bioethanol production efficiency. Therefore, improving the stress response ability of the strains is of great significance for industrial production of bioethanol. In this article, the response mechanisms of S. cerevisiae to various hydrolysate-derived stress factors including organic acids, furan aldehydes, and phenolic compounds have been reviewed. Organic acids mainly stimulate cells to induce intracellular acidification, furan aldehydes mainly break the intracellular redox balance, and phenolic compounds have a greater effect on membrane homeostasis. These damages lead to inadequate intracellular energy supply and dysregulation of transcription and translation processes, and then activate a series of stress responses. The regulation mechanisms of S. cerevisiae in response to these stress factors are discussed with regard to the cell wall/membrane, energy, amino acids, transcriptional and translational, and redox regulation. The reported key target genes and transcription factors that contribute to the improvement of the strain performance are summarized. Furthermore, the genetic engineering strategies of constructing multilevel defense and eliminating stress effects are discussed in order to provide technical strategies for robust strain construction. It is recommended that robust S. cerevisiae can be constructed with the intervention of metabolic regulation based on the specific stress responses. Rational design with multilevel gene control and intensification of key enzymes can provide good strategies for construction of robust strains. Graphical Abstract

show abstract

Newly identified genes contribute to vanillin tolerance in Saccharomyces cerevisiae

Cited by 18 publications

References 44 publications

Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

Production of xylitol by Saccharomyces cerevisiae using waste xylose mother liquor and corncob residues

Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

Contact Info

Product

Resources

About