Next-Generation Sequencing as a Tool to Detect Vaginal Microbiota Disturbances during Pregnancy

Sroka-Oleksiak, Agnieszka; Pabian, Wojciech; Gurgul, Artur; Kapusta, Przemysław; Ludwig-Słomczyńska, Agnieszka H.; Wołkow, Paweł; Brzychczy-Włoch, Monika

doi:10.3390/microorganisms8111813

Cited by 17 publications

(17 citation statements)

References 51 publications

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“…Both stool and oral swab samples were frozen at −80 • C until further processing. According to our previously published protocols of the proceedings, storage at −80 • C does not affect the results [21,22]. The bacterial DNA is very stable for a very long period of time under these conditions.…”

Section: Collection Of Swab and Fecal Samplesmentioning

confidence: 90%

Does Postoperative Oral and Intestinal Microbiota Correlate with the Weight-Loss Following Bariatric Surgery?—A Cohort Study

Stefura

Zapała

Stój³

et al. 2020

JCM

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The composition of the gastrointestinal microbiota is associated with obesity. The aim of this study was to verify if, six months after bariatric surgery, patients who achieve satisfying weight-loss after sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB) have a different composition of oral and intestinal microbiota in comparison with those who do not. This prospective cohort study was conducted between November 2018 and November 2020. Participants underwent either SG or RYGB and were allocated into: Group 1—participants who achieved a percentage of excess weight loss (%EWL) of ≥ 50%, and Group 2—patients with %EWL of < 50%. The %EWL was measured 6 months following surgery. At this time, oral swabs were obtained and stool samples were provided. The endpoint was the composition of the gut microbiota. Group 1 comprised 20 participants and Group 2 comprised 11 participants. Group 1 had oral microbiota more abundant in phylum Fusobacteria and intestinal microbiota more abundant in phylum Firmicutes. Group 2 had oral microbiota was more enriched in phylum Actinobacteria and intestinal microbiota was more enriched in phylum Bacteroidetes. The compositions of the microbiota of the oral cavity and large intestine 6 months after bariatric surgery are related to the weight-loss.

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Section: Collection Of Swab and Fecal Samplesmentioning

confidence: 90%

Does Postoperative Oral and Intestinal Microbiota Correlate with the Weight-Loss Following Bariatric Surgery?—A Cohort Study

Stefura

Zapała

Stój³

et al. 2020

JCM

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“…Although only about 25% of women are colonized (Russell et al, 2017), GBS is often considered a member of the host microbiota because carriage is usually asymptomatic. However, temporal carriage of vaginal GBS can be incredibly variable; wherein one patient may be persistently colonized whereas another may experience recurring or intermittent colonization (Brzychczy-Wloch et al, 2014;Sroka-Oleksiak et al, 2020). Additional studies are needed to understand how colonization varies temporally and across populations, particularly among pregnant and nonpregnant women.…”

Section: Gbs Vaginal Colonization and Persistence Maternal Gbs Colonization Is A Risk Factor For Subsequent Diseasementioning

confidence: 99%

“…The vaginal microbiota is composed of a wide array of taxa that varies geographically, by racial or ethnic group, throughout the menstrual cycle, and during pregnancy (Sroka-Oleksiak et al, 2020). Many taxa, including Prevotella bivia, Veillonella spp., Eubacterium siraeum, and Staphylococcus spp.…”

Section: Interactions Between the Vaginal Microbiome And Gbs Are Complexmentioning

confidence: 99%

“…Lactobacilli can have antagonistic effects on the growth of GBS and host cell interactions in vitro (Bodaszewska et al, 2010;Ortiz et al, 2014;Marziali et al, 2019;He et al, 2020). While in human observational vaginal microbiome studies, the relationship between Lactobacillus-replete microbiomes and GBS colonization is less clear (Kubota et al, 2002;Altoparlak et al, 2004;Daniel Johannes Rönnqvist et al, 2006;Brzychczy-Wloch et al, 2014;Rick et al, 2017), there is evidence that reduced taxonomic diversity and Lactobacillus dominance are possible mechanisms of microbiome-based protection against GBS during pregnancy (Sroka- Oleksiak et al, 2020). An exception is CST IV, which encompasses a more diverse and dysbiotic assortment of non-Lactobacillus anaerobes accompanied by increased vaginal pH (Ravel et al, 2011).…”

Section: Vaginal Lactobacilli: Key Species That Limit Gbs Colonization and Persistencementioning

confidence: 99%

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Bacterial and Host Determinants of Group B Streptococcal Vaginal Colonization and Ascending Infection in Pregnancy

Brokaw

Furuta

Dacanay

et al. 2021

Front. Cell. Infect. Microbiol.

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Group B streptococcus (GBS) is a gram-positive bacteria that asymptomatically colonizes the vaginal tract. However, during pregnancy maternal GBS colonization greatly predisposes the mother and baby to a wide range of adverse outcomes, including preterm birth (PTB), stillbirth, and neonatal infection. Although many mechanisms involved in GBS pathogenesis are partially elucidated, there is currently no approved GBS vaccine. The development of a safe and effective vaccine that can be administered during or prior to pregnancy remains a principal objective in the field, because current antibiotic-based therapeutic strategies do not eliminate all cases of invasive GBS infections. Herein, we review our understanding of GBS disease pathogenesis at the maternal-fetal interface with a focus on the bacterial virulence factors and host defenses that modulate the outcome of infection. We follow GBS along its path from an asymptomatic colonizer of the vagina to an invasive pathogen at the maternal-fetal interface, noting factors critical for vaginal colonization, ascending infection, and vertical transmission to the fetus. Finally, at each stage of infection we emphasize important host-pathogen interactions, which, if targeted therapeutically, may help to reduce the global burden of GBS.

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“…There is also an increasing awareness of the importance of maternal health during pregnancy for infant development and health, both before and after birth [ 13 ]. Importantly, these life stage events appear to be governed by particular interactions (immune and dietary) that ‘select’ beneficial microbes such as Bifidobacterium [ 4 , 14 , 15 ]. Species in this genus are prevalent in the gastrointestinal tract of mothers in the later stages of pregnancy and can represent up to 80 % of the total microbiome in healthy infants [ 14 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

The Pregnancy and EARly Life study (PEARL) - a longitudinal study to understand how gut microbes contribute to maintaining health during pregnancy and early life

et al. 2021

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Background The early life period represents the first step in establishing a beneficial microbial ecosystem, which in turn affects both short and longer-term health. Changes during pregnancy influence the neonatal microbiome; through transmission of maternal microbes during childbirth, and beyond, through nutritional programming. However, in-depth exploration of longitudinal maternal-infant cohorts, with sampling of multiple body sites, complemented by clinical and nutritional metadata, and use of cutting-edge experimental systems are limited. The PEARL study will increase our knowledge of; how microbes (including viruses/phages, bacteria, fungi and archaea) change in composition and functional capacity during pregnancy; transmission pathways from mother to infant; the impact of various factors on microbial communities across pregnancy and early life (e.g. diet), and how these microbes interact with other microbes and modulate host processes, including links to disease onset. Methods PEARL is a longitudinal observational prospective study of 250 pregnant women and their newborns, with stool and blood samples, questionnaires and routine clinical data collected during pregnancy, labour, birth and up to 24 months post birth. Metagenomic sequencing of samples will be used to define microbiome profiles, and allow for genus, species and strain-level taxonomic identification and corresponding functional analysis. A subset of samples will be analysed for host (immune/metabolite) molecules to identify factors that alter the host gut environment. Culturing will be used to identify new strains of health-promoting bacteria, and potential pathogens. Various in vitro and in vivo experiments will probe underlying mechanisms governing microbe-microbe and microbe-host interactions. Discussion Longitudinal studies, like PEARL, are critical if we are to define biomarkers, determine mechanisms underlying microbiome profiles in health and disease, and develop new diet- and microbe-based therapies to be tested in future studies and clinical trials. Trial registration This study is registered in the ClinicalTrials.gov Database with ID: NCT03916874.

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Next-Generation Sequencing as a Tool to Detect Vaginal Microbiota Disturbances during Pregnancy

Cited by 17 publications

References 51 publications

Does Postoperative Oral and Intestinal Microbiota Correlate with the Weight-Loss Following Bariatric Surgery?—A Cohort Study

Does Postoperative Oral and Intestinal Microbiota Correlate with the Weight-Loss Following Bariatric Surgery?—A Cohort Study

Bacterial and Host Determinants of Group B Streptococcal Vaginal Colonization and Ascending Infection in Pregnancy

The Pregnancy and EARly Life study (PEARL) - a longitudinal study to understand how gut microbes contribute to maintaining health during pregnancy and early life

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