Next-Generation Sequencing for Evaluating the Soil Nematode Diversity and Its Role in Composting Processes

Abstract

Biodiversity within composting systems involves a variety of microorganisms including nematodes. In the research, nematode populations were monitored within six simultaneously operating composting processes. These processes involved varying proportions of feedstock materials. The primary objective was to evaluate the consistency of nematode community succession patterns across the composting processes over a time of 3 months. During the study, samples were taken every month to isolate nematodes, determine the population density of the five trophic groups (per genus) and determine the dominant nematode species. It was shown that the bacterial-feeding community maintained dominance, while the fungus-feeding nematodes gradually increased in dominance as the maturation process progressed. The presence of predatory nematodes Mononchoides which were initially absent, along with the total absence of parasitic nematodes in the late stages of waste stabilization, serves as strong evidence for the reliable evaluation of the biodegradable waste processing level. Based on the obtained results, it is evident that the succession of nematode communities holds promise as a reliable method for evaluating compost maturity.

Influence of Eisenia fetida on the Nematode Populations during Vermicomposting Process

“…methodology described in the paper by Zapałowska et al [20]. Briefly, total D extracted using a DNA Mini Kit (Syngen Biotech, Wrocław, Poland), follow manufacturer's protocol.…”

“…The compost was prepared using different municipal wastes in various mass proportions (kg by mass). The composting process was conducted according to the methodology described in the paper by Zapałowska et al [20].…”

“…DNA extraction and libraries preparation were conducted according to the methodology described in the paper by Zapałowska et al [20]. Briefly, total DNA was extracted using a DNA Mini Kit (Syngen Biotech, Wrocław, Poland), following the manufacturer's protocol.…”

“…Community composition and taxonomic affiliations of the obtained sequences were performed by the software pipeline CCMetagen v1.2.3. The processing and analysis of sequencing data were conducted following the methodology described in the paper by Zapałowska et al [20], using the criteria for taxonomic assignment in CCMetagen as follows: species-level similarity threshold of 99.00%, genuslevel of 95.00%, family-level of 90.00%, order-level of 85.00%, class-level of 75.00%, and phylum-level of 55.00% [24].…”

The Impact of Using Different Types of Compost on the Growth and Yield of Corn

“…The soil was slightly acidic (pH 1N KCl 6.1), the content of phosphorus and magnesium was average (P 2 O 5 14 Compost fertilization utilized the substrate generated in the experiment, as detailed by Zapałowska et al [50]. The mentioned compost was prepared using different types of municipal waste in various mass proportions (kg by mass): The municipal waste was distributed among six bioreactors, each with a thermally insulated plastic cube holding 1 m 3 .…”