Next-Generation Sequencing Technologies: Approaches and Applications for Crop Improvement

Singh, A. K.; Ramakrishna, Goriparthi; Kaila, Tanvi; Saxena, Swati; Sharma, Sandhya; Gaikwad, Ambika B.; Abdin, Malik Zainul; Gaikwad, Kishor

doi:10.1007/978-1-0716-2533-0_3

Cited by 4 publications

(1 citation statement)

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“…It used to be too expensive to make labels for orphan crops. But thanks to progress in DNA sequencing, genetic engineering of important food varieties, PCR-based selection methods, and next-generation sequencing, this is now easier and costs less (Saini et al, 2020;Singh et al, 2022). Over a few years ago, molecular genetics and biotechnology have largely focused on improving crop varieties for human and animal use and biofuel and aesthetic purposes (Sainger et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Advancements in Genomic Technologies and Their Impact on Crop Improvement and Breeding Methods

REHMAN,

ABBAS,

ARSHAD

et al. 2024

Bull. Biol. All. Sci. Res.

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Recent advances in genome sequencing of essential crop plants allow genotype and phenotype data integration in crop development. Advanced statistical methods identified quantitative trait genes. Genomic prediction has been used successfully in breeding animals and is now also used in breeding plants. Biometric statistics, genome-wide markers, and phenotyping enhance gene discovery. This makes biotechnology agricultural plant solutions possible. Improved fertilizer efficiency requires precise plant phenotyping in multiple habitats and seasons, which was previously expensive. DNA sequencing, genetic engineering, and PCR-based marker-assisted selection have made it cheaper. New methods like next-generation sequencing can target climate-responsive crop development. It examines Oryza sativa L. flower opening and closing molecularly and hybrid breeding success in diverse plant types. It discusses CRISPR/Cas9 for crop trait improvement and metabolic studies for Triticum aestivum L. quality group determination. Genetic analysis should use species-specific techniques, according to the study. A Zea mays L. callus induction and growth study examined how media and growth conditions affect callus development. Another drought-stressed Triticum aestivum L. cultivar gene expression study suggests employing RNA editing to respond to environmental stress. PCR-based markers have helped scientists find important genes in landraces that have changed to survive harsh farming conditions, giving them targets for crop growth.

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Section: Introductionmentioning

confidence: 99%