Next-Generation Sequencing to Elucidate Adaptive Stress Response and Plantaricin Genes among
            <i>Lactobacillus Plantarum</i>
            Strains

Jabbar, Zuriat; Ikram-ul-Haq,; Mukhtar, Hamid; Tayyeb, Asima; Manzoor, Asma

doi:10.2217/fmb-2019-0158

Cited by 9 publications

(4 citation statements)

References 81 publications

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“…For example, a deletion was detected within the pst11A (c.13_16delAAAA; p.Lys5fs), dprA (c.134delC; p.His46fs), and aapA (c.1865delA; p.Gln622fs) genes in the UTNGt21A genome only. The PTS system is essential for sugar metabolism [ 3 ], while DprA protects against incoming foreign DNA [ 44 ]. AapA is an adherence-associated mucus-binding protein, which contributes to the persistence of bacteria in the human gut, thus exerting probiotic effects [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…The plantaricin genes are arranged in operons plnABCD , plnEFI, plnJKLR, plnMNOP , and plnGHSTUVWX [ 3 ]. Genome annotation analysis revealed the presence of a common two-peptide bacteriocin (plnEF) and several genes encoding for the ABC transport system.…”

Section: Resultsmentioning

confidence: 99%

“…Bioprospecting tropical plants to search for beneficial endophytic microorganisms that produce novel biotechnological molecules remains of interest [ 1 , 2 ]. Microorganisms associated with plants are subjected to constant metabolic and environmental interactions; however, the diversity of the produced molecules is linked to the host chemical composition [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Decoding the Gene Variants of Two Native Probiotic Lactiplantibacillus plantarum Strains through Whole-Genome Resequencing: Insights into Bacterial Adaptability to Stressors and Antimicrobial Strength

Tenea

2022

Genes

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In this study, whole-genome resequencing of two native probiotic Lactiplantibacillus plantarum strains—UTNGt21A and UTNGt2—was assessed in order to identify variants and perform annotation of genes involved in bacterial adaptability to different stressors, as well as their antimicrobial strength. A total of 21,906 single-nucleotide polymorphisms (SNPs) were detected in UTNGt21A, while 17,610 were disclosed in the UTNGt2 genome. The comparative genomic analysis revealed a greater number of deletions, transversions, and transitions within the UTNGt21A genome, while a small difference in the number of insertions was detected between the strains. A divergent number of types of variant annotations were detected in both strains, and categorized in terms of low, moderate, and high modifier impact on the protein effectiveness. Although both native strains shared common specific genes involved in the stress response to the gastrointestinal environment, which may qualify as a putative probiotic (bile salt, acid, temperature, osmotic stress), they were different in their antimicrobial gene cluster organization, with UTNGt21A displaying a complex bacteriocin gene arrangement and dissimilar gene variants that might alter their defense mechanisms and overall inhibitory capacity. The genome comparison revealed 34 and 9 genomic islands (GIs) in the UTNGt21A and UTNGt2 genomes, respectively, with the overrepresentation of genes involved in defense mechanisms and carbohydrate utilization. In addition, pan-genome analysis disclosed the presence of various strain-specific genes (shell genes), suggesting a high genome variation between strains. This genome analysis illustrates that the bacteriocin signature and gene variants reflect a niche-inherent pattern. These extensive genomic datasets will guide us to understand the potential benefits of the native strains and their utility in the food or pharmaceutical sectors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decoding the Gene Variants of Two Native Probiotic Lactiplantibacillus plantarum Strains through Whole-Genome Resequencing: Insights into Bacterial Adaptability to Stressors and Antimicrobial Strength

Tenea

2022

Genes

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“…In the current study, a significant down-regulation of the pln A genes and a significant up-regulation of plnD gene, responsible for encoding antimicrobial peptide plantaricin were observed in L. plantarum 14,917 with GOS in comparison to glucose. As plantaricin production of L. plantarum is based on the complicated operons system [35][36][37], the impact of GOS on plantaricin production remains unclear. Future research aimed at measuring plantaricin production could aid in clarifying the GOS effects of L. plantarum.…”

Section: Synbiotics Effect Of Gos and L Plantarum 14917 On Caries Con...mentioning

confidence: 99%

Anti-cariogenic Properties of Lactobacillus plantarum in the Utilization of Galacto-Oligosaccharide

et al. 2023

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Ecological approaches can help to correct oral microbial dysbiosis and drive the advent and persistence of a symbiotic oral microbiome, which benefits long-term dental caries control. The aim of this study was to investigate the impact of the prebiotic Galacto-oligosaccharide (GOS) on the growth of probiotics L. plantarum 14,917 and its effect on the inhibitory ability of L. plantarum 14,917 against the growth of Streptococcus mutans and Candida albicans in an in vitro model. Single-species growth screenings were conducted in TSBYE broth with 1% glucose and 1–5% GOS. Interaction experiments were performed using duo- and multi-species models with inoculation of 105 CFU/mL S. mutans, 103 CFU/mL C. albicans, and 108 CFU/mL L. plantarum 14,917 under 1%, 5% GOS or 1% glucose. Viable cells and pH changes were measured. Real-time PCR was utilized to assess expression of C. albicans and S. mutans virulence genes. Six replicates were used for each group. Student’s t-test, one-way ANOVA, and Kruskal-Wallis were employed to compare the outcomes of different groups. GOS significantly inhibited the growth of C. albicans and S. mutans in terms of growth quantity and speed when the two strains were grown individually. However, GOS did not affect the growth of L. plantarum 14,917. Moreover, 1% and 5% GOS enhanced the anti-fungal performance of L. plantarum 14,917 in comparison to 1% glucose. GOS as the carbon source resulted in a less acidic environment in the C. albicans and S. mutans duo-species model and multispecies model where L. plantarum 14,917 was added. When GOS was utilized as the carbohydrate substrate, S. mutans and C. albicans had a significant reduction in the expression of the HWP1, ECE1, atpD, and eno genes (p < 0.05). To our knowledge, this is the first study that reported the ability of GOS to neutralize S. mutans-C. albicans high caries of medium pH and to disrupt virulence gene expression. Moreover, as a prebiotic, GOS augmented the inhibitory ability of L. plantarum against C. albicans in vitro. The current study revealed the anti-caries potential of prebiotics GOS and shed light on novel caries prevention strategies from the perspective of prebiotics and probiotics. These findings provide a rationale for future biofilm or clinical studies to elucidate the effect of GOS on modulating oral microbiota and caries control.

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Key Stress Response Mechanisms of Probiotics During Their Journey Through the Digestive System: A Review

Castro-López

Romero-Luna

Garcı́a

et al. 2022

Probiotics & Antimicro. Prot.

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Next-Generation Sequencing to Elucidate Adaptive Stress Response and Plantaricin Genes among Lactobacillus Plantarum Strains

Cited by 9 publications

References 81 publications

Decoding the Gene Variants of Two Native Probiotic Lactiplantibacillus plantarum Strains through Whole-Genome Resequencing: Insights into Bacterial Adaptability to Stressors and Antimicrobial Strength

Decoding the Gene Variants of Two Native Probiotic Lactiplantibacillus plantarum Strains through Whole-Genome Resequencing: Insights into Bacterial Adaptability to Stressors and Antimicrobial Strength

Anti-cariogenic Properties of Lactobacillus plantarum in the Utilization of Galacto-Oligosaccharide

Key Stress Response Mechanisms of Probiotics During Their Journey Through the Digestive System: A Review

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