NFATc1 and TNFα expression in giant cell lesions of the jaws

Amaral, Fabrício Rezende; Brito, João Artur Ricieri; Perdigão, Paôlla Freitas; Carvalho, Vinicius Magalhães; Souza, Paulo Eduardo Alencar; Gomez, Marcus V.; Marco, Luiz; Gomez, Ricardo Santiago

doi:10.1111/j.1600-0714.2009.00855.x

Cited by 16 publications

(22 citation statements)

References 31 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the giant cells of these lesions do not have proliferative activity, and this prompted some authors to conclude that they are formed by cytoplasmic fusion and lose their proliferative ability during the transition from mononucleated to a multinucleated state [6][7][8]. Regarding the nature of the giant cells, it has also been suggested that they belong to the osteoclast lineage [7,9], and recently, two studies by our group strengthened this idea showing a strong expression of NFATc1, an osteoclasteogenesis factor, in these lesions [10,11].…”

Section: Introductionmentioning

confidence: 78%

Quantitative expression analysis of apoptotic/antiapoptotic genes and association with immunolocalization of BAX and BCL-2 in peripheral and central giant cell lesions of the jaws

et al. 2011

Self Cite

View full text Add to dashboard Cite

Central giant cell lesion (CGCL) and peripheral giant cell lesion (PGCL) of the jaws are characterized by multinucleated osteoclast-like giant cells in a background of mononuclear cells. While mononuclear cells retain proliferative activity in both lesions, giant cells are Ki-67 negative. This observation raised the theory that giant cells are formed by cytoplasmic fusion of mononuclear cells, and also that these lesions are of reactive nature. As the giant cells are not proliferating in CGCL and PGCL, apoptosis of such cells should be investigated. We investigated the transcription of BAX and BCL-2 mRNAs in six fresh samples of CGCL and six fresh samples of PGCL by qRT-PCR (quantitative reverse transcription PCR) and used immunohistochemistry to demonstrate the localization of these proteins, as well as caspase 3 active in six paraffin-embedded samples of CGCL and nine paraffin-embedded samples of PGCL. While both groups showed increased expression of BAX and BCL-2 mRNA, PGCL showed a higher apoptotic index (ratio BAX/BCL-2) than CGCL. The three proteins investigated were expressed almost exclusively in the cytoplasm of giant cells. To further confirm apoptotic activity, we performed TUNEL analysis in the same samples of the immunohistochemistry and found a higher positivity in the giant cells of PGCL compared to the giant cells of CGCL. Our results show increased expression of apoptotic-related genes in both PGCL and CGCL and that the giant cells are probably the main source of these events. Also, it raises a hypothesis that differences in the apoptotic activity might be associated with the different clinical behavior of CGCL and PGCL.

show abstract

Section: Introductionmentioning

confidence: 78%

Quantitative expression analysis of apoptotic/antiapoptotic genes and association with immunolocalization of BAX and BCL-2 in peripheral and central giant cell lesions of the jaws

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Results of our previous study showed an increased transcription of NFATc1 together with the positive immunostaining of this protein mainly in the nucleus of the multinucleated giant cell lesions of the jaws (13). In the present study, the immunoexpression of NFATc1 was compared in different lesions containing multinucleated giant cells.…”

Section: Discussionmentioning

confidence: 99%

“…The immunohistochemistry protocols used are described elsewhere (13). Briefly, paraffin-embedded tissue sections were incubated with NFATc1 antiserum (diluted 1:50, Clone 7A6, SantaCruz Biotechnology Inc., Santa Cruz, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Increased expression of NFATc1 in giant cell lesions of the jaws, cherubism and brown tumor of hyperparathyroidism

et al. 2011

Self Cite

View full text Add to dashboard Cite

Abstract.A variety of diseases of the jaws may present multinucleated giant cells. These diseases include central giant cell lesions (CGCL), peripheral giant cell lesions (PGCL), brown tumor of hyperparathyroidism (BTH), and cherubism. The multinucleated giant cells in these lesions are osteoclast-like. Since NFATc1 plays a significant role in osteoclast differentiation, the present study aimed to compare the expression of NFATc1 in CGCL, PGCL, BTH and cherubism. A total of 14 formalin-fixed and paraffin-embedded tissue samples of CGCL (n=4), PGCL (n=5), BTH (n=3) and cherubism (n=2) were included in the study. An immunohistochemical analysis was performed to investigate the NFATc1 protein.The majority of giant cells in all of the cases were positive for nuclear NFATc1 and the immunostaining pattern was similar in all of the groups. Although our study supports the hypothesis that giant cell accumulation in PGCL, CGCL, BTH and cherubism is mediated by NFATc1, functional studies are required to investigate this hypothesis.

show abstract

“…CGCL and PGCG are composed of mononuclear stromal cells that mimic osteoclast-precursors and MGCs that mimic differentiated osteoclasts [10]. NFATcI expression is also increased in both of these lesions [11]. They differ, however, in that CGCL arises centrally within bone, while PGCG is a gingival soft tissue lesion.…”

Section: Discussionmentioning

confidence: 97%

Central Giant Cell Lesion

Valentine¹,

Nelson²

2011

Head and Neck Pathol

View full text Add to dashboard Cite

A classic case of central giant cell lesion (CGCL) is presented with emphasis on clinical, radiologic, and histologic features. The differential is discussed including peripheral giant cell granuloma, brown tumor of hyperparathyroidism, and giant cell tumor of bone. The molecular pathway of osteoclastogenesis is selectively reviewed and applied to suggest possible etiologies of the giant cell lesions. CGCL syndromes and treatment are also discussed.

show abstract

NFATc1 and TNFα expression in giant cell lesions of the jaws

Abstract: The development of giant cells lesions of the jaws and cherubism are possibly mediated by overexpression of NFAT in the nucleus of the multinucleated cells.

Cited by 16 publications

References 31 publications

Quantitative expression analysis of apoptotic/antiapoptotic genes and association with immunolocalization of BAX and BCL-2 in peripheral and central giant cell lesions of the jaws

Quantitative expression analysis of apoptotic/antiapoptotic genes and association with immunolocalization of BAX and BCL-2 in peripheral and central giant cell lesions of the jaws

Increased expression of NFATc1 in giant cell lesions of the jaws, cherubism and brown tumor of hyperparathyroidism

Central Giant Cell Lesion

Contact Info

Product

Resources

About