Nfya-1 functions as a substrate of ERK-MAP kinase during Caenorhabditis elegans vulval development

Aklilu, Segen; Krakowiak, Michelle; Frempong, Abena; Wilson, Katherine L.; Powers, Christy; Fantz, Douglas A.

doi:10.1016/j.cdev.2021.203757

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…We chose to focus on nfya-1 because it showed the strongest phenotype. In C. elegans , loss of nfya-1 has previously been shown to affect vulva development, sensory neuron specification, and expression of specific cell fate transcription factors (Deng et al, 2007; Milton et al, 2013; Aklilu et al, 2022; Heo et al, 2022). NFYA-1/NFY-A is a subunit of the conserved, ubiquitously expressed trimeric repressor complex NF-Y/CBF.…”

Section: Resultsmentioning

confidence: 99%

A sex-specific switch in a single glial cell patterns the apical extracellular matrix

Fung

Tan

Kolotuev

et al. 2023

Preprint

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Apical extracellular matrix (aECM) constitutes the interface between every tissue and the outside world. It is patterned into diverse tissue-specific structures through unknown mechanisms. Here, we show that a male-specific genetic switch in a single C. elegans glial cell patterns the aECM into a ~200 nm pore, allowing a male sensory neuron to access the environment. We find that this glial sex difference is controlled by factors shared with neurons (mab-3, lep-2, lep-5) as well as previously unidentified regulators whose effects may be glia-specific (nfya-1, bed-3, jmjd-3.1). The switch results in male-specific expression of a Hedgehog-related protein, GRL-18, that we discover localizes to transient nanoscale rings at sites of aECM pore formation. Blocking male-specific gene expression in glia prevents pore formation, whereas forcing male-specific expression induces an ectopic pore. Thus, a switch in gene expression in a single cell is necessary and sufficient to pattern aECM into a specific structure.

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Section: Resultsmentioning

confidence: 99%

A sex-specific switch in a single glial cell patterns the apical extracellular matrix

Fung

Tan

Kolotuev

et al. 2023

Preprint

View full text Add to dashboard Cite

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A sex-specific switch in a single glial cell patterns the apical extracellular matrix

Fung,

Tan,

Kolotuev

et al. 2023

Current Biology

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The role(s) of NF-Y in development and differentiation

Dolfini,

Imbriano,

Mantovani

2024

Cell Death Differ

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NF-Y is a conserved sequence-specific trimeric Transcription Factor -TF- binding to the CCAAT element. We review here the role(s) in development, from pre-implantation embryo to terminally differentiated tissues, by rationalizing and commenting on genetic, genomic, epigenetic and biochemical studies. This effort brings to light the impact of NF-YA isoforms on stemness and differentiation, as well as binding to distal vs promoter proximal sites and connections with selected TFs.

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Nfya-1 functions as a substrate of ERK-MAP kinase during Caenorhabditis elegans vulval development

Cited by 3 publications

References 34 publications

A sex-specific switch in a single glial cell patterns the apical extracellular matrix

A sex-specific switch in a single glial cell patterns the apical extracellular matrix

A sex-specific switch in a single glial cell patterns the apical extracellular matrix

The role(s) of NF-Y in development and differentiation

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