NHS-ester functionalized poly(PEGMA) brushes on silicon surface for covalent protein immobilization

Yang, Yong; Ma, Yongzheng; Qin, Ming; Ma, Xiaojing; Wang, Chen; Feng, Xisheng

doi:10.1016/j.colsurfb.2008.06.015

Cited by 31 publications

(35 citation statements)

References 17 publications

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“…173 For example, in a demonstration of this principle, immunoglobin ͑IgG͒ has been immobilized on the NHS-bearing poly͑OEGMA͒ brushes on silicon substrates. 174 This was explored as a prototype for a sensor based on binding of fluorescently labeled anti-IgG antibody in a micropatterned array. 175 Given the successful demonstration that poly͑OEGMA͒ brushes resist in vitro and in vivo adsorption of proteins and that immobilization of specific adhesion sequences can be used to promote cell adhesion and functions, we have recently extended this approach to explore the surface modification of titanium with saccharide-based polymer brushes.…”

Section: Discussionmentioning

confidence: 99%

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

et al. 2009

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This review focuses on the surface modification of substrates with self-assembled monolayers (SAMs) and polymer brushes to tailor interactions with biological systems and to thereby enhance their performance in bioapplications. Surface modification of biomedical implants promotes improved biocompatibility and enhanced implant integration with the host. While SAMs of alkanethiols on gold substrates successfully prevent nonspecific protein adsorption in vitro and can further be modified to tether ligands to control in vitro cell adhesion, extracellular matrix assembly, and cellular differentiation, this model system suffers from lack of stability in vivo. To overcome this limitation, highly tuned polymer brushes have been used as more robust coatings on a greater variety of biologically relevant substrates, including titanium, the current orthopedic clinical standard. In order to improve implant-bone integration, the authors modified titanium implants with a robust SAM on which surface-initiated atom transfer radical polymerization was performed, yielding oligo(ethylene glycol) methacrylate brushes. These brushes afforded the ability to tether bioactive ligands, which effectively promoted bone cell differentiation in vitro and supported significantly better in vivo functional implant integration.

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Section: Discussionmentioning

confidence: 99%

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

et al. 2009

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“…Biofouling caused great environmental penalty in many fields, more than $78 billion per year was cost due to medical devices contamination in USA, and the loss of ship and underwater facilities caused by biofouling is more than $50 billion per year in the world . Many studies illustrated surface contamination can be decreased by surface modification . Economic loss and human health issues associated to biofilm formation, which raise an urgent need for developing anti‐biofilm method.…”

Section: Introductionmentioning

confidence: 99%

“…An antimicrobial peptide, magainin II (MAG II), was discovered and isolated from the granular gland of the skin of the African frog, Xenopus laevis . MAG II has a broad spectrum of antimicrobial activity against Gram‐positive and Gram‐negative bacteria . Magainin I (MAG I, own different amino acid at positions 10 and 22) can be chemically grafted on solid surface for obtaining antimicrobial surface .…”

Section: Introductionmentioning

confidence: 99%

A biofilm resistance surface yielded by grafting of antimicrobial peptides on stainless steel surface

Cao

Yuan

Xiao

et al. 2018

Surface & Interface Analysis

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Anti‐biofilm formation on the surface is a severe issue in medical implants, hull surface, and food industry. Antimicrobial peptide, magainin II, was covalently bound to stainless steel surfaces through multi‐step modification. The untreated and modified samples were analyzed by SEM‐EDS, XPS, and contact angle, respectively, which indicated the peptide was immobilized on the surfaces. The antimicrobial tests of modified samples were conducted using Staphylococcus aureus and Escherichia coli, and the results revealed that peptide modified surface decreased the biofilm and bacteria quantity of stainless steel surface.

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“…Piranha solution results in a highly hydrophilic silicon surface and sets OH groups into the surface. OH groups are suitable for covalently binding of the proteins [21]. The silicon channels are then functionalized by covalent attachment of rabbit polyclonal antibodies with GPTS (glycidoxypropyltrimethoxysilane) [22] by using a modification of the procedure described by Kim et al [23].…”

Section: Memristors Bio-functionalizationmentioning

confidence: 99%

Memristive-biosensors: A new detection method by using nanofabricated memristors

Carrara

Sacchetto

Doucey

et al. 2012

Sensors and Actuators B: Chemical

124

101

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NHS-ester functionalized poly(PEGMA) brushes on silicon surface for covalent protein immobilization

Cited by 31 publications

References 17 publications

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

A biofilm resistance surface yielded by grafting of antimicrobial peptides on stainless steel surface

Memristive-biosensors: A new detection method by using nanofabricated memristors

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