Niche Construction and Exploitation by Agrobacterium: How to Survive and Face Competition in Soil and Plant Habitats

Dessaux, Yves; Faure, Denis

doi:10.1007/82_2018_83

Cited by 25 publications

(25 citation statements)

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“…Opines produced in tumors therefore serve as a selective nutrient source for tumorigenic bacterium and induce conjugative transfer of Ti plasmids (reviewed in Dessaux et al. 1998; Farrand 1998; Dessaux and Faure 2018a). On the other hand, our results suggested that the strain Colt5.8 is also able to catabolize opines and exploit crown gall tumors.…”

Section: Discussionmentioning

confidence: 99%

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Evolutionary Relatedness and Classification of Tumor-Inducing and Opine-Catabolic Plasmids in Three Rhizobium rhizogenes Strains Isolated from the Same Crown Gall Tumor

Kuzmanović

Puławska

2019

Genome Biology and Evolution

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Plasmids play a crucial role in the ecology of agrobacteria. In this study, we sequenced tumor-inducing (Ti) and opine-catabolic (OC) plasmids in three Rhizobium rhizogenes ( Agrobacterium biovar 2) strains isolated from the same crown gall tumor on “Colt” cherry rootstock and conducted comparative genomic analyses. Tumorigenic strains C5.7 and C6.5 carry nopaline-type Ti plasmids pTiC5.7/pTiC6.5, whereas the nonpathogenic strain Colt5.8 carries the nopaline-type OC plasmid pOC-Colt5.8. Overall, comparative genomic analysis indicated that pTiC5.7/pTiC6.5 and related Ti plasmids described before (pTiC58 and pTi-SAKURA) originate from a common ancestor, although they have diverged during evolution. On the other hand, plasmid pOC-Colt5.8 was most closely related to the well-known OC plasmid pAtK84b; however, analysis suggested that they had different evolutionary histories and seem to share a more distant common ancestor. Although the reconstruction of the evolutionary history of Ti and OC plasmids is still speculative, we hypothesized that nopaline-type Ti plasmid might originate from the nopaline-type OC plasmid. Our results suggested that OC plasmids are widespread and closely associated with crown gall tumors. Finally, we proposed a thorough scheme for classification of Ti and OC plasmids that is based on separate comparative analysis of each functional element of the plasmid studied.

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Section: Discussionmentioning

confidence: 99%

“…Opines are specific organic compounds that are mainly used as selective nutrient sources by a pathogen (Dessaux et al. 1998; Dessaux and Faure 2018a).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary Relatedness and Classification of Tumor-Inducing and Opine-Catabolic Plasmids in Three Rhizobium rhizogenes Strains Isolated from the Same Crown Gall Tumor

Kuzmanović

Puławska

2019

Genome Biology and Evolution

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“…Pathogenic agrobacteria can catabolize opines using genes carried by their Ti plasmid. Opines are mainly used as carbon and nitrogen sources, but can also be used as phosphate or sulfur sources (Dessaux et al, 1998; Flores-Mireles et al, 2012; Dessaux and Faure, 2018). One of the features of the Ti plasmid is that it confers the bacterium that harbors it the possibility to catabolize a specific type of opine determined by the Ti plasmid-type (Gordon and Christie, 2014).…”

Section: Bacteria Find Trophic Resources and Toxic Compounds Inside Tmentioning

confidence: 99%

“…Around 40 different types of opines have been characterized; some of them trigger the transfer of the Ti plasmid from one bacterium to another, enhancing pathogenicity and contributing to the persistence of pathogenic bacteria in the environment (Dessaux et al, 1998; Flores-Mireles et al, 2012). These properties confer opines a central role in the Agrobacterium -infected plant interaction (Guyon et al, 1980; Dessaux and Faure, 2018). According to the “opine concept,” the presence of opines improves the competitiveness of the bacteria able to catabolize them at the expense of the bacteria unable to do so.…”

Section: Introductionmentioning

confidence: 99%

Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors

et al. 2019

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The study of pathogenic agents in their natural niches allows for a better understanding of disease persistence and dissemination. Bacteria belonging to the Agrobacterium genus are soil-borne and can colonize the rhizosphere. These bacteria are also well known as phytopathogens as they can cause tumors (crown gall disease) by transferring a DNA region (T-DNA) into a wide range of plants. Most reviews on Agrobacterium are focused on virulence determinants, T-DNA integration, bacterial and plant factors influencing the efficiency of genetic transformation. Recent research papers have focused on the plant tumor environment on the one hand, and genetic traits potentially involved in bacterium-plant interactions on the other hand. The present review gathers current knowledge about the special conditions encountered in the tumor environment along with the Agrobacterium genetic determinants putatively involved in bacterial persistence inside a tumor. By integrating recent metabolomic and transcriptomic studies, we describe how tumors develop and how Agrobacterium can maintain itself in this nutrient-rich but stressful and competitive environment.

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“…Rhizobiaceae is a family of bacteria that include soil-dwelling and plant associated bacteria. integrates into the plant chromosomes (1)(2)(3)(6)(7)(8)(9)(10)(11). The T-DNA segment encodes for metabolic and hormone-producing enzymes that hijack the plant to produce custom energy sources that only Agrobacterium can use (9,10).…”

Section: Introductionmentioning

confidence: 99%

Induction of AmpC-mediated β-lactam resistance requires a single lytic transglycosylase inAgrobacterium tumefaciens

Figueroa-Cuilan

Howell

Richards

et al. 2020

Preprint

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The remarkable ability of Agrobacterium tumefaciens to transfer DNA to plant cells has allowed the generation of important transgenic crops. One challenge of A. tumefaciens-mediated transformation is eliminating the bacteria after plant transformation to prevent detrimental effects to plants and the release of engineered bacteria to the environment. Here we use a reverse genetic approach to identify genes involved in ampicillin resistance with the goal of utilizing these antibiotic-sensitive strains for plant transformations. We show that treating A. tumefaciens C58 with ampicillin led to increased β-lactamase production, a response dependent on the broad-spectrum β-lactamase AmpC and its transcription factor AmpR. Loss of the putative ampD orthologue, atu2113, led to constitutive production of AmpC-dependent β-lactamase activity and ampicillin resistance. Finally, one cell wall remodeling enzyme, MltB3, was necessary for the AmpC-dependent β-lactamase activity and its loss elicited ampicillin and carbenicillin sensitivity in the A. tumefaciens C58 and GV3101 strains. Furthermore, GV3101 ΔmltB3 transforms plants with comparable efficiency to wildtype but can be cleared with sub-lethal concentrations of ampicillin. The functional characterization of the genes involved in the inducible ampicillin resistance pathway of A. tumefaciens constitutes a major step forward in efforts to reduce the intrinsic antibiotic resistance of this bacterium.IMPORTANCEAgrobacterium tumefaciens, a significant biotechnological tool for transgenic plant lines, is highly resistant to a wide variety of antibiotics, posing challenges for various applications. One challenge is the efficient elimination of A. tumefaciens from transformed plant tissue without using levels of antibiotics that are toxic to the plants. Here, we present the functional characterization of genes involved in β-lactam resistance in A. tumefaciens. Knowledge about proteins that promote or inhibit β-lactam resistance will enable the development of strains to improve the efficiency of Agrobacterium-mediated plant genetic transformations. Effective removal of Agrobacterium from transformed plant tissue has the potential to maximize crop yield and food production, improving the outlook for global food security.

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Niche Construction and Exploitation by Agrobacterium: How to Survive and Face Competition in Soil and Plant Habitats

Cited by 25 publications

References 208 publications

Evolutionary Relatedness and Classification of Tumor-Inducing and Opine-Catabolic Plasmids in Three Rhizobium rhizogenes Strains Isolated from the Same Crown Gall Tumor

Evolutionary Relatedness and Classification of Tumor-Inducing and Opine-Catabolic Plasmids in Three Rhizobium rhizogenes Strains Isolated from the Same Crown Gall Tumor

Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors

Induction of AmpC-mediated β-lactam resistance requires a single lytic transglycosylase inAgrobacterium tumefaciens

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