Nigrosin eosin as a stain for differentiating live and dead spermatozoa

Abstract

1. The proportion of stained spermatozoa in a ram semen-nigrosin-eosin mixture has been found to increase with the time of exposure to the stain. Five minutes is considered to be a suitable interval for practical use.2. The value obtained for the proportion of dead cells can be considerably affected by the selection of fields that are easy to count; fields should be chosen at random.3. The variation between, counts on the same smear is much larger than that expected for binomial proportions. This may be due partly to the ‘clumping’ of the dead spermatozoa and partly to the difficulties of interpretation caused by the presence of many partially stained cells.4. A significant difference was found between the estimates of the proportion dead obtained by the two experimental workers; this is thought to be due to a difference of interpretation of ‘stained’ and ‘unstained’.5. Tables are given by which to determine the approximate accuracy of counts made on a range of numbers of spermatozoa, smears and subsamples, separate tables being necessary for ram, boar and bull semen.

Heparin Binding Sites in the Human Spermatozoa Membrane

“…Special care was taken to prevent membrane damage before the incubation procedure. Observation of an aliquot of the washed sperm cells showed good motility and more than 80% of viable cells [ 2 ] . The washed sperm cells were resuspended to a final concentration of 5 x lo7 cells/ml in the incubation medium containing (4.16- Duplicate reaction mixtures were incubated under several different experimental conditions and then the binding process was terminated by chilling the tubes in melting ice and fixing the cells by the addition of 3 volumes of 0.1 M cacodylate buffer, pH 7.3, containing 1% paraformaldehyde plus 2.5% glutaraldehyde.…”

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

“…Live and abnormal sperm percentages were assayed by staining smears with Eosin-Nirgosin [18]. A total of 200 sperm cells were examined unsystematically.…”

The Effect of Freeze Drying Using Different Media and Storage Temperatures on Some Parameters of Buffalo Bull Spermatozoa

“…Smears were stained with Eosin-Nigrosin (Campbell et al, 1956) morphologically abnormal sperm percent. A total of 200 sperm cells will examined randomly.…”