Ninhydrin Based Visible Spectrophotometric Determination of Gemigliptin

Abstract

A simple method is described to determine the amount of gemigliptin in bulk and tablet formulation by visible spectrophotometry. Basis of the proposed method is the reaction of the primary amine present on gemigliptin with ninhydrin in alkaline pH (alkaline borate buffer) medium to produce a purple color (Ruhemann's purple) which has maximum absorption at 558 nm. The method was validated as per the current ICH guidelines. The obtained regression equation (y = 0.0148x+0.0011) in the range of 5-30 μg mL -1 has a good correlation coefficient (> 0.999). As the method does not require any separation, it is rapid and simple. The recovery levels of the drug were in the range of 99.73 -99.96. This method is a green method as it no organic solvents were employed.

Visible Spectrophotometric Determination of Gemigliptin using Charge Transfer Complex

“…Out of the various reactions involved in spectrophotometric assay of pharmaceutical drugs, charge transfer reaction is proved to be highly sensitive as well as acceptable method, in addition to reactions involving to ion pair [9][10][11][12][13][14][15] and oxidation [16][17][18][19][20] . The complex formed between electron donor and acceptor compound yields a new absorption band with a precise l max pertaining to that complex.…”