NIR‐Emitting Quantum Dot‐Encoded Microbeads through Membrane Emulsification for Multiplexed Immunoassays

Wang, Xiebing; Wang, Gang; Li, Wanwan; Zhao, Bingxia; Xing, Bin; Leng, Yuankui; Dou, Hongjing; Sun, Kang; Shen, Lisong; Yuan, Xiangliang; Li, Jiyu; Kim, Sun; Han, Jianyong; Xiao, Huasheng; Li, Yue; Huang, Peng; Chen, Xiaoyuan

doi:10.1002/smll.201203156

Cited by 34 publications

(33 citation statements)

References 45 publications

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“…3(b), a 3D barcode library based on FL1-FL4-FS signals of a flow cytometer (Beckman Coulter FC500) was generated by incorporating 520 nm QDs and 680 nm QDs into differentially-sized polymer microspheres. 30 NIR (near-infrared) QDs (650–800 nm) with wide PL spectra (FWHM > 50 nm) can also be utilized, and a “single wavelength” encoding method 66 was developed to take advantage of QDs in the NIR region. With this method, the spectra of NIR QDs span over FL4 and FL5 fluorescent channels of the flow cytometer.…”

Section: Spectrometrically or Optically Encoded Microspheres With mentioning

confidence: 99%

“…However, it was found that iron oxide nanocrystals decreased QD fluorescence intensities by absorbing excitation light and QD fluorescence emissions. 13 NIR-emitting QDs and Fe 3 O 4 nanoparticles were capsulated into PSMA microspheres 66 resulting in a similar decrease in fluorescence intensity, but to a lesser extent than visible light-emitting QDs due to the minimal NIR absorption of magnetic nanoparticles. Jun et al 125 coated SERS dots onto magnetic microspheres to produce magnetic SERS barcodes.…”

Section: Magnetic Nanoparticle-based Suspension Arraysmentioning

confidence: 99%

“…(c-3) Fluorescence barcode matrices of 685, 708, and 745 nm QD-encoded microbeads on a flow cytometric FL5-FL4 plot diagram. 12,30,66,68 …”

Section: Figmentioning

confidence: 99%

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Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

et al. 2015

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Spectrometrically or optically encoded microsphere based suspension array technology (SAT) is applicable to the high-throughput, simultaneous detection of multiple analytes within a small, single sample volume. Thanks to the rapid development of nanotechnology, tremendous progress has been made in the multiplexed detecting capability, sensitivity, and photostability of suspension arrays. In this review, we first focus on the current stock of nanoparticle-based barcodes as well as the manufacturing technologies required for their production. We then move on to discuss all existing barcode-based bioanalysis patterns, including the various labels used in suspension arrays, label-free platforms, signal amplification methods, and fluorescence resonance energy transfer (FRET)-based platforms. We then introduce automatic platforms for suspension arrays that use superparamagnetic nanoparticle-based microspheres. Finally, we summarize the current challenges and their proposed solutions, which are centered on improving encoding capacities, alternative probe possibilities, nonspecificity suppression, directional immobilization, and “point of care” platforms. Throughout this review, we aim to provide a comprehensive guide for the design of suspension arrays, with the goal of improving their performance in areas such as multiplexing capacity, throughput, sensitivity, and cost effectiveness. We hope that our summary on the state-of-the-art development of these arrays, our commentary on future challenges, and some proposed avenues for further advances will help drive the development of suspension array technology and its related fields.

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Section: Spectrometrically or Optically Encoded Microspheres With mentioning

confidence: 99%

Section: Magnetic Nanoparticle-based Suspension Arraysmentioning

confidence: 99%

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Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

et al. 2015

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“…The coating of the microbeads with CRA1 was based on the previously published method (23). The coating of the microbeads with CRA1 was based on the previously published method (23).…”

Section: Cytometric Bead Immunoassay Of Sfceriamentioning

confidence: 99%

Establishment of a novel quantum dots‐encoded microbead‐based flow cytometric method for quantification of soluble FcεRIα in serum

Lin

et al. 2017

Cytometry Pt A

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The soluble form of the transmembrane glycoprotein, FcεRIα which corresponds to the high-affinity receptor for IgE, is found in serum. Growing evidence suggests the pathogenic role of IgE and FcεRI in systemic lupus erythematosus (SLE). The goal of this study is to develop a sensitive and standardized cytometric assay for quantification of sFcεRIα. A membrane emulsification technique was utilized to incorporate CuInS /ZnS quantum dots and Fe O nanoparticles into poly (styrene-co-maleic anhydride) microbeads. The beads were then carboxylated and coated with capture antibody monoclonal anti-human FcεRIα. This antibody binds to FcεRIα but does not block the binding of FcεRIα to IgE. After incubation with standards or serum samples, the microbeads were incubated with excessive native human IgE, followed by incubation with Phycoerythrin (PE) conjugated anti-human IgE. The resulting quantum dot microbeads were gated, and sFcεRIα quantification was analyzed based on PE fluorescence intensity. The method exhibited good linearity (R > 0.99), and the limit of detection was established at 0.29 ng/mL with the dynamic range of up to 200 ng/mL. The precision of the assay validated by intra- and inter-assay variability met the acceptance criteria with the mean recovery falling within 80-110% of the theoretical concentration and a corresponding CV < 20%. We tested 149 serum samples which 89 were from SLE patients and 60 were from healthy volunteers. For the first time, we detected an increased sFcεRIα level in the serum of SLE patients, which was confirmed by a commercial ELISA kit. Compared to ELISA, this novel method is more sensitive and efficient. It allows for the simple comparative analysis of sFcεRIα levels in health and disease. © 2017 International Society for Advancement of Cytometry.

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“…A total of twelve articles were analyzed [6][7][8][9][10][11][12][13][14][15][16][17], of which ten are devoted to the use of different techniques for creating the stable polymer microsphere/colloidal QDs hybrid structures [6][7][8][9][10][11][12][13][14][15] and two articles are devoted specifically to sensors of temperature [16,17]. It is need to point out, that one of them [16] What about our research group, it is necessary to mention the two publications [18,19], preceding the development of the model of the nanoscale biosensor of temperature presented in this article.…”

Section: Physbiosymp17mentioning

confidence: 99%

Design of the Model of Ratiometric Polymer Nanobiothermometer Based on Quantum Dots

Mokrova¹,

Сизова²,

Mochalov³

et al. 2018

KEn

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To solve many modern biological and biotechnological tasks it is necessary to realize strictly control and regulation of temperature of the cells and their organelles. This tasks include control of various exo-and endothermic reactions, monitoring of tissues' and individual cell's temperature in in vitro researches and in vivo procedures such as the hyperthermia procedure that used for cancer treatment. The today known methods of measuring and controlling of temperature at the cellular level can not provide the necessary level of locality and accuracy due to too big size and heightened sensitivity to external factors. The real alternative of existing today methods is nanoscale temperature biosensor operating on a ratiometric principle and based on the composite structure from polymers and colloidal quantum dots. In this paper we present a working model and plan of investigation of ratiometric nanoscale polymer nanobiothermometer based on quantum dots.

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NIR‐Emitting Quantum Dot‐Encoded Microbeads through Membrane Emulsification for Multiplexed Immunoassays

Cited by 34 publications

References 45 publications

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

Establishment of a novel quantum dots‐encoded microbead‐based flow cytometric method for quantification of soluble FcεRIα in serum

Design of the Model of Ratiometric Polymer Nanobiothermometer Based on Quantum Dots

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