Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis

Chamizo-Ampudia, Alejandro; Sanz-Luque, Emanuel; Llamas, Ángel; Galván, Aurora; Fernández, Emilio

doi:10.1016/j.tplants.2016.12.001

Cited by 354 publications

(239 citation statements)

References 97 publications

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“…H 2 O 2 can activate various defense mechanisms through the redox signaling pathway . NO can be formed from NO 2 − and NO 3 − , which is also involved in the activation of the protective processes under stress conditions . The fact that PAW#2—which contains more H 2 O 2 , and less NO 2 − and NO 3 − than PAW#1—has increased the germination rate and shoot length already after short‐term treatment, whereas PAW#1 only after the long‐term one, suggests that H 2 O 2 could be more important in the growth process under stress conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The above‐mentioned studies indicate that the synergistic effect between the NO 3 − and H 2 O 2 is responsible for the physiological effects of the PAW. NO can be formed from NO 3 − in a reaction catalyzed by nitrate reductase . NO, together with H 2 O 2 and other ROS, as signal transducers, may be involved in the mediation of the effect of the PAW on metabolism in plants .…”

Section: Introductionmentioning

confidence: 99%

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Plasma‐activated water to improve the stress tolerance of barley

Gierczik

Vukušić

Kovács

et al. 2020

Plasma Processes & Polymers

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It is shown that plasma‐activated water (PAW) improves tolerance against combined low temperature and hypoxia stresses during germination. Due to PAW's effect on the antioxidant system, PAW pretreatment mitigates the reducing effect on plant growth of the hydroponically applied NaCl. PAW pretreatment reduces the salt‐stress‐induced decrease of the cysteine and γ‐glutamylcysteine content and improves the plant's recovery after NaCl stress. PAW pretreatment also modifies the NaCl‐induced changes in carotenoid content, especially, β‐carotenes and xanthophylls. These compounds, together with glutathione can reduce salt‐induced damage through the removal of excess reactive oxygen species, which could be the basis of the protective effect of PAW.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plasma‐activated water to improve the stress tolerance of barley

Gierczik

Vukušić

Kovács

et al. 2020

Plasma Processes & Polymers

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“…It will be interesting and significant to identify the ARC proteins and investigate the effects of Mo on ARC proteins in wheat in future studies. Besides, Chamizo-Ampudia et al (2017) reviewed that the Mo-enzymes aldehyde oxidase (AO), sulfite oxidase (SO) and xanthine dehydrogenase (XDH) are also involved in the NO production in vitro. However, to our best knowledge, there are no researches confirm that AO, SO and XDH induce NO production in plants.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Mediates Molybdenum-Induced Antioxidant Defense in Wheat under Drought Stress

Tan

et al. 2017

Front. Plant Sci.

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Molybdenum (Mo) has been reported to alleviate drought stress by enhancing antioxidant defense in plants, but the underlying mechanism remains unclear. Here, we hypothesized that Mo mediates nitric oxide (NO)-induced antioxidant defense through Mo-enzymes, particularly by nitrate reductase (NR) in wheat under drought stress. The 30-day-old wheat seedlings cultivated in -Mo (0 μM Mo) and +Mo (1 μM Mo) Hoagland solutions were detached and then pretreated with Mo-enzyme inhibitors, NO scavengers, NO donors or their combinations according to demands of complementary experiment under 10% polyethylene glycol 6000 (PEG)-stimulated drought stress (PSD). Mo supplementation increased the activities and transcripts of antioxidant enzymes, decreased H2O2 and MDA contents, and elevated NO production, implying that Mo-induced antioxidant defense may be related to NO signal. Complementary experiment showed that NO production was induced by Mo, while suppressed by Mo-enzyme inhibitors and NO scavengers, but restored by NO donors, suggesting that Mo-induced increase of NO production may be due to the regulation by Mo-enzymes. Further experiment indicated that the increased activities and transcripts of antioxidant enzymes induced by Mo were suppressed by Mo-enzyme inhibitors and NO scavengers, and NO donors could eliminate their suppressing effects. Moreover, Mo application increased NR activity and inhibitors of Mo-enzymes inhibited NR activity in wheat leaves under PSD, suggesting that NR might involve in the regulation of Mo-induced NO production. These results clearly indicate that NO mediates Mo-induced antioxidant defense at least partially through the regulation of NR.

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“…The first pathway involves NR which catalyzes the reduction of nitrite to NO. NR is a main NO source during abiotic stress and in the signaling pathway that is triggered by abscisic acid leading to stomatal closure (Chamizo-Ampudia et al, 2017). The second one involves a putative NOS.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of E3 Ubiquitin Ligase Gene AdBiL Contributes to Resistance against Chilling Stress and Leaf Mold Disease in Tomato

et al. 2017

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Ubiquitination is a common regulatory mechanism, playing a critical role in diverse cellular and developmental processes in eukaryotes. However, a few reports on the functional correlation between E3 ubiquitin ligases and reactive oxygen species (ROS) or reactive nitrogen species (RNS) metabolism in response to stress are currently available in plants. In the present study, the E3 ubiquitin ligase gene AdBiL (Adi3 Binding E3 Ligase) was introduced into tomato line Ailsa Craig via Agrobacterium-mediated method. Transgenic lines were confirmed for integration into the tomato genome using PCR. Transcription of AdBiL in various transgenic lines was determined using real-time PCR. Evaluation of stress tolerance showed that T1 generation of transgenic tomato lines showed only mild symptoms of chilling injury as evident by higher biomass accumulation and chlorophyll content than those of non-transformed plants. Compared with wild-type plants, the contents of AsA, AsA/DHA, GSH and the activity of GaILDH, γ-GCS and GSNOR were increased, while H2O2, O2.−, MDA, NO, SNOs, and GSNO accumulations were significantly decreased in AdBiL overexpressing plants in response to chilling stress. Furthermore, transgenic tomato plants overexpressing AdBiL showed higher activities of enzymes such as G6PDH, 6PGDH, NADP-ICDH, and NADP-ME involved in pentose phosphate pathway (PPP). The transgenic tomato plants also exhibited an enhanced tolerance against the necrotrophic fungus Cladosporium fulvum. Tyrosine nitration protein was activated in the plants infected with leaf mold disease, while the inhibition could be recovered in AdBiL gene overexpressing lines. Taken together, our results revealed a possible physiological role of AdBiL in the activation of the key enzymes of AsA–GSH cycle, PPP and down-regulation of GSNO reductase, thereby reducing oxidative and nitrosative stress in plants. This study demonstrates an optimized transgenic strategy using AdBiL gene for crop improvement against biotic and abiotic stress factors.

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Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis

Cited by 354 publications

References 97 publications

Plasma‐activated water to improve the stress tolerance of barley

Plasma‐activated water to improve the stress tolerance of barley

Nitric Oxide Mediates Molybdenum-Induced Antioxidant Defense in Wheat under Drought Stress

Overexpression of E3 Ubiquitin Ligase Gene AdBiL Contributes to Resistance against Chilling Stress and Leaf Mold Disease in Tomato

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