Nitric oxide alleviates oxidative damage in the green algaChlorella pyrenoidosa caused by UV-B radiation

K, Chen; Feng, Hongmei; M, Zhang; Wang, X

doi:10.1007/bf02931372

Cited by 52 publications

(34 citation statements)

References 26 publications

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“…A different response of catalase to UV-B has been reported in Nicotiana plumbaginifolia (Willekens et al 1994) and Zea mays (Boldt & Scandalios 1997) due to the presence of several isoforms and to the different metabolic functions. It has already been demonstrated that UV-B treatment can trigger upregulation of antioxidant enzymes (Chen et al 2003). Studies conducted by Yannarelli et al (2006) in soybean showed an increase of CAT activity under the lower UV-B doses demonstrating that this enzyme is up-regulated to safeguard normal cellular functions and survival.…”

Section: Discussionmentioning

confidence: 97%

Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativusL.)

Cantarello

Volpe

Azzolin

et al. 2005

Journal of Plant Interactions

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Exposure to UV-B at ambient or enhanced levels is known to trigger a variety of responses in all living organisms, including higher plants. Here we show that in Cucumis sativus L. UV-B radiation affects enzyme activity of key oxydative pentose phosphate pathway (OPPP) enzymes glucose-6-phosphate dehydrogenase (G6P-DH) and 6-phosphogluconate dehydrogenase (6-PGlu-DH), of key phenolic compounds enzyme phenylalanine ammonia lyase (PAL) as well as erythrose-4-phosphate, tryptophan and tyrosine levels. Furthermore, we found an increased activity of antioxidant enzymes such as peroxidase (POX) and catalase (CAT) in treated plants, with respect to the controls. In order to confirm the biochemical results, we isolated total RNA from both controls and UV-B treated plants to be used for gene expression analysis. We demonstrated that UV-B increases the gene expression level of peroxidase (POX ), catalase (CAT ) and phenylalanine ammonia lyase (PAL ). Finally, our results are useful for understanding protective strategies against UV-B radiation and for elucidating what components are involved in stress-induced signals within the plant.

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Section: Discussionmentioning

confidence: 97%

Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativusL.)

Cantarello

Volpe

Azzolin

et al. 2005

Journal of Plant Interactions

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“…With regard to algae, it was found that both exogenous and endogenous nitric oxide can alleviate oxidative damage in the green alga Chlorella pyrenoidosa caused by UV-B radiation (Chen et al, 2003a). Research indicated that exogenous antioxidants including ASC, NAC, and SNP can protect algae under UV-B radiation (Chen et al, 2003a), but, with regard to the roles of exogenous antioxidants on the response to oxidant stress, there is also little information, especially about how they interact with endogenous antioxidant enzymes to protect organisms from oxidant stress (He and Ha¨der, 2002a;Laspina et al, 2005;Malanga et al, 1999;Morley et al, 2003). In this study, these issues were investigated with aims to: (1) investigate the responses of photosynthesis and antioxidant enzymes of desert Nostoc sp.…”

Section: Introductionmentioning

confidence: 99%

The involvement of the antioxidant system in protection of desert cyanobacterium Nostoc sp. against UV-B radiation and the effects of exogenous antioxidants

Wang

Chen

et al. 2008

Ecotoxicology and Environmental Safety

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“…In algae NO formation was reported to be involved in phototaxis (39) and stress responses (10,41), and in fungi it is related to processes such as growth (40,63) and formation of fruiting bodies (59).…”

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confidence: 99%

Rehydration of the LichenRamalina laceraResults in Production of Reactive Oxygen Species and Nitric Oxide and a Decrease in Antioxidants

Weissman¹,

Garty²,

Hochman

2005

Appl Environ Microbiol

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Lichens are slow-growing associations of fungi and unicellular green algae or cyanobacteria. They are poikilohydric organisms whose lifestyle in many cases consists of alternating periods of desiccation, with low metabolic activity, and hydration, which induces increase in their metabolism. Lichens have apparently adapted to such extreme transitions between desiccation and rehydration, but the mechanisms that govern these adaptations are still poorly understood. In this study, the effect of rehydration on the production of reactive oxygen species and nitric oxide as well as low-molecular-weight antioxidants was investigated with the lichen Ramalina lacera. Rehydration of R. lacera resulted in the initiation of and a rapid increase in photosynthetic activity. Recovery of photosynthesis was accompanied by bursts of intracellular production of reactive oxygen species and nitric oxide. Laser-scanning confocal microscopy using dichlorofluorescein fluorescence revealed that formation of reactive oxygen species following rehydration was associated with both symbiotic partners of the lichen. The rate and extent of reactive oxygen species production were similar in the light and in the dark, suggesting a minor contribution of photosynthesis. Diaminofluorescein fluorescence, indicating nitric oxide formation, was detected only in fungal hyphae. Activities associated with rehydration did not have a deleterious effect on membrane integrity as assessed by measurement of electrolyte leakage, but water-soluble low-molecular-weight antioxidants decreased significantly.Lichens are slow-growing associations of fungi (mycobionts) and photosynthetic partners (photobionts) that may be unicellular green algae or cyanobacteria. They occupy a vast range of habitats and substrates and produce unique biochemical compounds that have made them useful to humans as food, dyes, medicines, fine perfumes, and poisons (51). In addition, many lichen species are sensitive to air pollution, and this feature, along with their capability to accumulate mineral elements far above their need, makes them ideal bioindicators and biomonitors of air pollution (see, e.g., references 7, 19, and 52).Metabolic activation of molecular oxygen frequently results in production of reactive oxygen species (ROS) (25). ROS are formed by normal metabolic activities such as respiration and photosynthesis, but their production is enhanced during stresses such as nutrient limitation, exposure to xenobiotics, or desiccation and rehydration. To evade the potential damaging effects of ROS, cells have evolved protection mechanisms, including antioxidant enzymes, such as superoxide dismutase and catalase, as well as low-molecular-weight antioxidants, such as glutathione, ascorbic acid, tocopherol, and carotenoids (25).Nitric oxide (NO) is an intra-and intercellular signaling molecule involved in the regulation of diverse biochemical and physiological processes. In algae NO formation was reported to be involved in phototaxis (39) and stress responses (10, 41), and in fungi it is...

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Nitric oxide alleviates oxidative damage in the green algaChlorella pyrenoidosa caused by UV-B radiation

Cited by 52 publications

References 26 publications

Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativusL.)

Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativusL.)

The involvement of the antioxidant system in protection of desert cyanobacterium Nostoc sp. against UV-B radiation and the effects of exogenous antioxidants

Rehydration of the LichenRamalina laceraResults in Production of Reactive Oxygen Species and Nitric Oxide and a Decrease in Antioxidants

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