Nitric oxide mediates abscisic acid induced light-tolerance in leaves of tall fescue under high-light stress

Xu, Yuefei; Fu, Juanjuan; Chu, Xitong; Sun, Yanan; He, Zhenli; Hu, Tianming

doi:10.1016/j.scienta.2013.07.042

Cited by 23 publications

(25 citation statements)

References 43 publications

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“…Knight et al 11 have also reported that lanthanum (La) and EGTA inhibit the salt-and mannitol-induced (Ca 2+ ) cyt elevations in Arabidopsis. In a previous study, Xu et al 18 have reported that ABA protects tall fescue plant from oxidative injuries by promoting NO release (via activating NOS) thereby triggering the activities of antioxidant enzymes. Therefore, also in order to study the possible link between Ca 2+ and NO signalling, As + Ca treated Brassica seedlings were treated with NO scavenger: c-PTIO and synthase inhibitor: l-NAME.…”

Section: Resultsmentioning

confidence: 99%

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Singh

Parihar

Prasad

2020

Sci Rep

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In this study, Ca 2+ mediated no signalling was studied in response to metalloid (As) stress in Brassica seedlings. Arsenic toxicity strongly suppressed the growth (fresh weight, root and shoot length), photosynthetic pigments, Chl a fluorescence indices (Kinetic traits: F v , F m , F v /F o , F m /F o , ФP o or F v /F m , Ψ o , ФE o , PI ABS , Area and N and redox status (AsA/DHA and GSH/GSSG ratios) of the cell; whereas energy flux traits: ABS/RC, TR o /RC, ET o /RC and DI o /RC along with F o , F o /F v , F o /F m , ФD o and S m ) were enhanced. Further, addition of EGTA (Ca 2+ scavenger) and Lacl 3 (plasma membrane ca 2+ channel blocker) to As + ca; while c-ptio (no scavenger) and l-nAMe (no synthase inhibitor) to As + Snp treated seedlings, siezed recovery on above parameters caused due to Ca 2+ and NO supplementation, respectively to As stressed seedlings thereby indicating their signalling behaviour. Further, to investigate the link between ca 2+ and NO, when c-ptio and l-nAMe individually as well as in combination were supplemented to As + ca treated seedlings; a sharp inhibition in above mentioned traits was observed even in presence of ca 2+ , thereby signifying that NO plays crucial role in Ca 2+ mediated signalling. In addition, As accumulation, ROS and their indices, antioxidant system, NO accumulation and thiol compounds were also studied that showed varied results.Arsenic (As) exposure has become a major threat for world agriculture that causes adverse effect on crop productivity by inhibiting cell functioning. The metalloid (As) mainly arises from geothermal weathering of rocks and human activities 1 . The toxicity of As depends on As species. Among the two inorganic species, arsenite (As III ), which prevails in anaerobic environment, enters in plant system through aquaporin channels with greater affinity for thiol groups; while arsenate (As V ) prevalent in aerobic condition/ soil, being analogue of inorganic phosphate (iP), enters via inorganic phosphate transporters, competes with phosphate and replaces phosphate from ATP thereby affecting the energy metabolism of cell 2,3 . Arsenic accumulation causes growth suppression which involves many biochemical and physiological changes in plants including oxidative stress, injuries to membrane and thereby affecting redox state of the cell 4-6 , which is detrimental for plant survival under As toxicity. Additionally, As toxicity negatively regulates chlorophyll (Chl) biosynthesis, PS II photochemistry and ribulose 1,5-bisphosphate carboxylase/ oxygenase (RuBisCO) activity, thereby inhibiting photosynthetic efficiency 6-9 . Previous studies have explicated the prominence of upholding a favourable antioxidants level and redox status of cell to encounter the damage caused due to metalloid exposure 4-6 .Calcium (Ca 2+ ) is a pervasive and pivotal secondary messenger in signal transduction network 10 under both stressed and non-stressed situations. In several studies, it has been shown that Ca 2+ is involved in regulation of plant responses such as photosyn...

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Section: Resultsmentioning

confidence: 99%

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Singh

Parihar

Prasad

2020

Sci Rep

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“…Nitric oxide (NO), an important gaseous signaling molecule, plays a key role in regulating plant growth and developmental processes such as seed germination, cell death, de-etiolation, pathogen response, cell senescence, cell signal transduction, and programmed cell death (PCD). 13,14) Our recent studies indicated that NO pretreatment or He-Ne laser illumination activated plant antioxidant defenses in tall fescue seedlings during high saline conditions, 3,15) and we supposed that in improving plant adaptive responses to salt stress, there may be a similar correlation between NO and He-Ne laser irradiation. Thereby, the primary objective of the present study is to assess the interactive correlations between He-Ne laser irradiation-induced favorable biological effects and NO biosynthesis in vivo in plants through investigating the protective effect of He-Ne laser illumination on reactive oxygen species (ROS) level, plant antioxidant system and membrane injury in leaves of tall fescue seedlings, and further explore the role of endogenous NO molecule in the laser protective effects on salt-stressed seedlings.…”

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confidence: 85%

“…Houndog5) were surface sterilized with 6.0% sodium hypochlorite (v/v, NaClO) supplemented with 0.1% Tween-20 for 30 min, rinsed with distilled water for several times, and cultured on moistened gauze in Petri dishes for germination in a plant incubator (PGX-250B, AC 220 V/50 HZ, 600 W, Shanghai Fuma Laboratory Instrument Co., Ltd.) for 7d under darkness, at 25 ± 2°C, 70% relative humidity. After germination, seedlings were transplanted into black plastic containers containing suitable volume of Hoagland's nutrient solution, 14,15,21) and cultured hydroponically at relative humidity 70% conditions (day/night 24/20°C), a photoperiod 16/8 h day/night cycle, and photosynthetic photo flux density (PPFD) 100 μmol•m −2 •s −1 . The pH of Hoagland's nutrient solution was adjusted to 6.0-6.5 with 1 N HCl or 1 N NaOH.…”

Section: Methodsmentioning

confidence: 99%

“…Ion leakage determination. The relative ion leakage of leaves of tall fescue seedlings from different treatments was determined according to the method of Xu et al and Song et al 14,30) Lipid peroxidation assay. Lipid peroxidation was evaluated by the malondialdehyde (MDA) relative amounts in seedling leaves, which were measured by the method of Gao et al 3) Polyamine determination.…”

Section: Experimental Treatmentsmentioning

confidence: 99%

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Endogenous nitric oxide mediates He-Ne laser-induced adaptive responses in salt stressed-tall fescue leaves

Gao

Han

2016

Bioscience, Biotechnology, and Biochemistry

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The aim of this study was to investigate the role of endogenous nitric oxide in protective effects of He-Ne laser on salt stressed-tall fescue leaves. Salt stress resulted in significant increases of membrane injury, reactive oxygen species (ROS) production, polyamine accumulation, and activities of SOD, POD, and APX, while pronounced decreases of antioxidant contents, CAT activity and intracellular Ca(2+) concentration in seedlings leaves. He-Ne laser illumination caused a distinct alleviation of cellular injury that was reflected by the lower MDA amounts, polyamine accumulation and ROS levels at the stress period. In contrast, the laser treatment displayed a higher Ca(2+) concentration, antioxidant amounts, NO release, antioxidant enzyme, and NOS activities. These responses could be blocked due to the inhibition of NO biosynthesis by PTIO (NO scavenger) or LNNA (NOS inhibitor). The presented results demonstrated that endogenous NO might be involved in the progress of He-Ne laser-induced plant antioxidant system activation and ROS degradation in order to enhance adaptive responses of tall fescue to prolonged saline conditions.

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“…is the predominant, perennial, open-pollinated, cool-season turf and forage grass species grown or planted under diverse environmental conditions (Dong et al 2007;Xu et al 2010Xu et al , 2013. It is widely used for general purpose turf and low-maintenance grass cover, and plays an important role in environmental protection and livestock production in China (Wang and Ge 2005;Ma et al 2014).…”

Section: Introductionmentioning

confidence: 99%

The detoxification effects of He-Ne laser irradiation on cytotoxicity of cadmium sulfide nanoparticles (CdSNPs) in tall fescue seedlings

Gao

Han

2016

Can. J. Plant Sci.

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Because of their wide applications in manufacturing life science and medicine, nanoparticles (NPs) are becoming potential limiting factors for agricultural plant growth and productivity. Thus, development of some low cost and low risk methods for detoxifying or alleviating cytotoxicity of NPs on crops would be a proactive attractive solution. In this study, we investigated the ameliorating effect of He-Ne laser illumination of seedlings on cytotoxicity of cadmium sulfide nanoparticles (CdSNPs) in tall fescue seedlings. Physiological parameters and biochemical characteristics of 20-d-old seedlings with different times of laser illumination and graded doses of CdSNPs treatment, either alone or in combination, were measured. Cadmiun sulfide nanoparticles exhibited pronounced inhibitory effect on shoot and root growth, respectively. Accumulation of CdSNPs in plant root cells resulted in oxidative stress as detected by the formation of reactive oxygen species (ROS), which aggravate the cytotoxicity of CdSNPs on seedlings. Treatment of seedlings with CdSNPs alone also reduced antioxidant concentration, phytochelatins (PCs) content, enzymatic activities of phytochelatin synthase (PCS), and antioxidant enzymes, such as CAT, APX, GR, and POD, except SOD. However, He-Ne laser illumination prior to CdSNPs exposure alleviated the inhibitory effect on seedling growth and development through activation of tall fescue antioxidant systems due to induction of antioxidants biosynthesis and antioxidant enzyme activities. Furthermore, PCs and GSH induced by laser was also demonstrated to be involved in decreasing the cytotoxicity of CdSNPs through binding free cadmium ions release from CdSNPs. The interactive mechanisms between He-Ne lasers, plant antioxidant systems, and PCs biosynthesis pathways need be further investigated.

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Nitric oxide mediates abscisic acid induced light-tolerance in leaves of tall fescue under high-light stress

Cited by 23 publications

References 43 publications

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Endogenous nitric oxide mediates He-Ne laser-induced adaptive responses in salt stressed-tall fescue leaves

The detoxification effects of He-Ne laser irradiation on cytotoxicity of cadmium sulfide nanoparticles (CdSNPs) in tall fescue seedlings

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