Nitric oxide modulates ionic transport in the isolated intestine of the eel, Anguilla anguilla

Trischitta, F.; Pidalà, Patrizia; Faggio, Caterina

doi:10.1016/j.cbpa.2007.05.025

Cited by 23 publications

(23 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrogen abstraction from radical-cationic L-arginine by radical-anionic riboflavin occurs during the radical formation process upon the visible light irradiation. Gao et al found that the radicals generated on amine species also take part in the propagation of radicals, and therefore the amine species are covalently bonded to the polymer as a result [25]. In this study, the radicals, that initiate the polymerization reaction, are generated in both (-)-riboflavin and L-arginine.…”

Section: The Effect Of (-)-Riboflavin On the Photocrosslinking Of Dexmentioning

confidence: 87%

“…Therefore, L-arginine would be covalently bonded to the dextranmethacrylate hydrogel backbone. This would be very beneficial since L-arginine has many biological activities including nitric oxide formation [25][26][27].…”

Section: The Effect Of (-)-Riboflavin On the Photocrosslinking Of Dexmentioning

confidence: 99%

“…L-arginine promoted the photocrosslinking reaction in all concentrations. Gao et al reported the radical formation mechanism of (-)-riboflavin and amine species under the UV irradiation [25]. The scheme was reconstructed to show the mechanism of the radical formation of (-)-riboflavin and Larginine under the visible light source (Scheme 1).…”

Section: The Effect Of (-)-Riboflavin On the Photocrosslinking Of Dexmentioning

confidence: 99%

“…L-arginine and biodegradable polymers synthesized from arginine have also recently been used in gene delivery system because of its (+) charged amine groups and water solubility [26,27]. Biocompatibility and biodegradability are also important factors in adopting L-arginine in many biomedical matrix development [25][26][27]. Since hydrogel precursor solution should be a complete homogeneous solution, water-solubility of L-arginine also contributes to the selection of appropriate electron donor of (-)-riboflavin photoinitiation system.…”

Section: Introductionmentioning

confidence: 99%

“…We found that L-arginine can act as an electron donor because it has amine in its chemical structure ( Figure 2). L-arginine is an amino acid that has many biological activities [25][26][27]. It has been reported that L-arginine promotes the nitric oxide synthesis in the body and enhances the transportation into the cell.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Visible light induced dextran-methacrylate hydrogel formation using (−)-riboflavin vitamin B2 as a photoinitiator and L-arginine as a co-initiator

Kim

Chu

2009

Fibers Polym

View full text Add to dashboard Cite

The objective of this study is to examine the feasibility of using visible light to form gels from polysaccharide precursors. Hydrogel formation by visible light irradiation would be very beneficial because visible light is a benign light source and ready available when compared with other light sources such as UV. Dextran-methacylate was synthesized and photocrosslinked using (-)-riboflavin as a photoinitiator and L-arginine as a co-initiator under the visible light. The effect of various concentrations of (-)-riboflavin and L-arginine on the photocrosslinking of dextran-methacrylate hydrogel was investigated. The fabricated hydrogel was characterized by FT-IR and SEM. The photoinitiator [(-)-riboflavin] and co-initiator (L-arginine) as well as dextran precursor are completely biocompatible. The optimum condition for the biocompatible dextran-based hydrogel formation under the harmless light source (visible light) was elucidated in this study. In general, the (-)-riboflavin, 0.01-0.5 %, and L-arginine, 5-20 % of the weight of dextran-methacrylate were the best condition in forming dextran-based hydrogels under the visible light. The three-dimensional hydrogel structure was verified by SEM morphology of swollen hydrogels. Photocrosslinking under the visible light source would enlarge the applications of this type of photocrosslinking in the biomedical area (e.g., eyes or other light-sensitive organs).

show abstract

Section: The Effect Of (-)-Riboflavin On the Photocrosslinking Of Dexmentioning

confidence: 87%

Section: The Effect Of (-)-Riboflavin On the Photocrosslinking Of Dexmentioning

confidence: 99%

Section: The Effect Of (-)-Riboflavin On the Photocrosslinking Of Dexmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Visible light induced dextran-methacrylate hydrogel formation using (−)-riboflavin vitamin B2 as a photoinitiator and L-arginine as a co-initiator

Kim

Chu

2009

Fibers Polym

View full text Add to dashboard Cite

show abstract

Fabrication of a biodegradable polysaccharide hydrogel with riboflavin, vitamin B2, as a photo‐initiator and L‐arginine as coinitiator upon UV irradiation

Kim

Chu

2009

J Biomed Mater Res

View full text Add to dashboard Cite

The objective of this study is to develop a completely natural material based photo-initiation system for fabricating biologically compatible hydrogels, that is, free of any synthetic materials like conventional synthetic photo-initiators. The resulting biodegradable hydrogels would be completely free of conventional toxic synthetic substances. Dextran-based precursor was used as a model system for this study. The natural material based photo-initiation system used riboflavin (vitamin B2) as photo-initiator and L-arginine as a coinitiator. Dextran-methacrylate precursor was prepared to introduce photosensitive unsaturated moieties into dextran for subsequent photo-induced gel formation. Riboflavin alone could not initiate the photo-crosslinking reaction of the dextran methacrylate solution upon UV irradiation. L-arginine, which can act as an electron donor in the photo-initiation process, was used as a coinitiator. The optimum gel formulation conditions in terms of the concentrations of riboflavin and L-arginine as well as different pH media were extensively investigated. The dextran-methacrylate hydrogels were formed well and fast at low riboflavin concentration range (0.2-1 wt %), and L-arginine promoted the photo-initiation at all concentrations. The gelation was promoted most in the neutral pH and the least in the alkaline pH. The turbidity of the dextran-methacrylate hydrogel precursor solution affected the polymerization because UV penetration was significantly hindered as the opacity of the solution increased. The swelling property and structural visualization of the prepared hydrogels were also investigated.

show abstract

Nitric oxide synthase (NOS) inhibitor L‐NAME activates inducible NOS/NO system and drives multidimensional regulation of Na⁺/K⁺‐ATPase in ionocyte epithelia of immersion‐stressed air‐breathing fish (Anabas testudineus Bloch)

Peter

Gayathry

2021

J Exp Zool Pt A

View full text Add to dashboard Cite

Nitric oxide (NO) has been implicated in Na+ homeostatic control in water‐breathing fishes. It is, however, uncertain whether air‐breathing fish relies on NO to coordinate Na+/K+‐ATPase (NKA)‐driven Na+ transport during acute hypoxemia. We, thus, examined the action of nitric oxide synthase (NOS) inhibitor, L‐NAME on NO availability, inducible NOS (iNOS) protein abundance and the regulatory dynamics of NKA in osmoregulatory epithelia of Anabas testudineus kept at induced hypoxemia. As expected in nonstressed fish, in vivo L‐NAME (100 ng g−1) challenge for 30 min declined NO production in serum (40%) and osmoregulatory tissues (average 51.6%). Surprisingly, the magnitude of such reduction was less in hypoxemic fish after L‐NAME challenge due to the net gain of NO (average 23.7%) in these tissues. Concurrently, higher iNOS protein abundance was found in branchial and intestinal epithelia of these hypoxemic fish. In nonstressed fish, L‐NAME treatment inhibited the NKA activity in branchial and intestinal epithelia while stimulating its activity in renal epithelia. Interestingly in hypoxemic fish, L‐NAME challenge restored the hypoxemia‐inhibited NKA activity in branchial and renal epithelia. Similar recovery response was evident in the NKAα protein abundance in immunoblots and immunofluorescence images of branchial epithelia of these fish. Analysis of Nkaα1 isoform transcript abundance (Nkaα1a, α1b, α1c) also showed spatial and preferential regulation of Nkaα1 isoform switching. Collectively, the data indicate that L‐NAME challenge activates iNOS/NO system in the branchial ionocyte epithelia of hypoxemia‐stressed Anabas and demands multidimensional regulation of NKA to restore the Na+ transport rate probably to defend against acute hypoxemia.

show abstract

Nitric oxide modulates ionic transport in the isolated intestine of the eel, Anguilla anguilla

Cited by 23 publications

References 28 publications

Visible light induced dextran-methacrylate hydrogel formation using (−)-riboflavin vitamin B2 as a photoinitiator and L-arginine as a co-initiator

Visible light induced dextran-methacrylate hydrogel formation using (−)-riboflavin vitamin B2 as a photoinitiator and L-arginine as a co-initiator

Fabrication of a biodegradable polysaccharide hydrogel with riboflavin, vitamin B2, as a photo‐initiator and L‐arginine as coinitiator upon UV irradiation

Nitric oxide synthase (NOS) inhibitor L‐NAME activates inducible NOS/NO system and drives multidimensional regulation of Na⁺/K⁺‐ATPase in ionocyte epithelia of immersion‐stressed air‐breathing fish (Anabas testudineus Bloch)

Contact Info

Product

Resources

About

Nitric oxide modulates ionic transport in the isolated intestine of the eel, Anguilla anguilla

Cited by 23 publications

References 28 publications

Visible light induced dextran-methacrylate hydrogel formation using (−)-riboflavin vitamin B2 as a photoinitiator and L-arginine as a co-initiator

Visible light induced dextran-methacrylate hydrogel formation using (−)-riboflavin vitamin B2 as a photoinitiator and L-arginine as a co-initiator

Fabrication of a biodegradable polysaccharide hydrogel with riboflavin, vitamin B2, as a photo‐initiator and L‐arginine as coinitiator upon UV irradiation

Nitric oxide synthase (NOS) inhibitor L‐NAME activates inducible NOS/NO system and drives multidimensional regulation of Na+/K+‐ATPase in ionocyte epithelia of immersion‐stressed air‐breathing fish (Anabas testudineus Bloch)

Contact Info

Product

Resources

About

Nitric oxide synthase (NOS) inhibitor L‐NAME activates inducible NOS/NO system and drives multidimensional regulation of Na⁺/K⁺‐ATPase in ionocyte epithelia of immersion‐stressed air‐breathing fish (Anabas testudineus Bloch)