Nitric oxide prevents rat duodenal contractions induced by potentially noxious agents

Hellgren,; Flemström,; Nylander,

doi:10.1046/j.1365-201x.2000.00738.x

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…It also decrease in adipose tissue mass has a role to play in weight management. In addition, capsaicin affects various physiological functions which include intestinal peristalsis (Hellgren et al, 2000), gastroprotection (Szoscanyi and Bartho, 2001), temperature regulation (Nomoto et al, 2004), modulation of the energy metabolism (Kawada et al, 1986), immune status (Yu et al, 1998) and blood neuthrophils (Zhukova and Makarova, 2002). Capsaicin has also been found to exert protective effect against Salmonela enteritidis infection in laying hens (Vicente et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of Capsaicin Supplementation on Productive and Physiological Performance of Pekin Ducks During Summer Season

Ali¹,

Ahmed

El-Gabry³

2016

Egyptian Journal of Nutrition and Feeds

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he aim of this study was to evaluate the effect of using graded levels of capsaicin on growth performance, nutrients digestibility coefficients, serum metabolites, oxidative responses and intestinal digestive enzymes activity of Pekin ducks. One hundred and twenty 14d old Pekin ducks were randomly divided into four groups of 30 ducks. The first group was fed on the basal diet (control), while groups 2, 3 and 4 were given the basal diet supplemented with 50, 100 and 150 ppm capsaicin, respectively. The results showed significant improvement in body weight and feed conversion of ducks, particularly with the high level (150ppm) followed by those of the mid one (100pm). Moreover, supplemental capsaicin with different levels enhanced digestibility coefficient values especially with ether extract (EE) and nitrogen free extract (NFE) in Pekin ducks. The serum level of total proteins, globulin, high density lipoprotein (HDL), triiodothyronine (T3), thyroxin (T4) and antioxidant enzymes was significantly increased for ducks fed on diets contained either 100 or 150ppm capsaicin. But, the concentrations of total lipid, cholesterol, triglycerides, low density lipoprotein (LDL), glucose and malondialdehyde (MDA), which is the primary stable by-product of lipid peroxidation, were reduced. On the other hand, the albumin level didn't significantly affected by dietary capsaicin supplementation. A significant increase in the activities of amylase, lipase and trypsin enzymes was found throughout the small intestine portions with supplemental capsaicin. It is concluded that, capsaicin supplementation with 100ppm up to 150ppm was sufficient to enhance the growth performance traits, nutrients digestibility, thyroid and antioxidant system in growing Pekin ducks. In addition to, using these natural feed additives had clear favorable effect on enzymatic and microbiological profile in small intestine, without adverse effects on liver activity.

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Section: Introductionmentioning

confidence: 99%

Effects of Capsaicin Supplementation on Productive and Physiological Performance of Pekin Ducks During Summer Season

Ali¹,

Ahmed

El-Gabry³

2016

Egyptian Journal of Nutrition and Feeds

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“…However, relatively little is known about the involvement of intramural neurohumoral regulation of the intestinal permeability in the intact animal. Recent experiments from our laboratory have shown that luminal perfusion of the rat duodenum with various solutions such as hydrochloric acid , ethanol and hypo‐ and hyperosmolar fluids affects the blood‐to‐lumen clearance of 51 Cr‐EDTA, a reliable technique to assess changes in epithelial paracellular permeability. It was further demonstrated that the hypotonicity‐induced increases in duodenal paracellular permeability are mediated via serotonin (5‐HT) acting on 5‐HT 3 and 5‐HT 4 receptors as well as by changes in neural activity possibly suggesting that the enteric nervous system participates in the regulation of tight junction epithelial permeability.…”

Section: Introductionmentioning

confidence: 99%

Melatonin decreases duodenal epithelial paracellular permeability via a nicotinic receptor–dependent pathway in rats in vivo

Sommansson

Nylander

Sjöblom

2012

Journal of Pineal Research

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Intestinal epithelial intercellular tight junctions (TJs) provide a rate-limiting barrier restricting passive transepithelial movement of solutes. TJs are highly dynamic areas, and their permeability is changed in response to various stimuli. Defects in the intestinal epithelial TJ barrier may contribute to intestinal inflammation or leaky gut. The gastrointestinal tract may be the largest extrapineal source of endogenous melatonin. Melatonin released from the duodenal mucosa is a potent stimulant of duodenal mucosal bicarbonate secretion (DBS). The aim of this study was to elucidate the role of melatonin in regulating duodenal mucosal barrier functions, including mucosal permeability, DBS, net fluid flux, and duodenal motor activity, in the living animal. Rats were anesthetized with thiobarbiturate, and a ~30-mm segment of the proximal duodenum with an intact blood supply was perfused in situ. Melatonin and the selective melatonin receptor antagonist luzindole were perfused luminally or given intravenously. Effects on permeability (blood-to-lumen clearance of (51)Cr-EDTA), DBS, mucosal net fluid flux, and duodenal motility were monitored. Luminal melatonin caused a rapid decrease in paracellular permeability and an increase in DBS, but had no effect on duodenal motor activity or net fluid flux. Luzindole did not influence any of the basal parameters studied, but significantly inhibited the effects of melatonin. The nonselective and noncompetitive nicotinic acetylcholine receptor antagonist mecamylamine abolished the effect of melatonin on duodenal permeability and reduced that on DBS. In conclusion, these findings provide evidence that melatonin significantly decreases duodenal mucosal paracellular permeability and increases DBS. The data support the important role of melatonin in the neurohumoral regulation of duodenal mucosal barrier.

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“…Noxious agents have been shown to disrupt this barrier and increase the clearance of large molecules from blood to lumen (14,21). Different research groups have shown that inhibition of endogenous NO production leads to an increase in mucosal permeability (22,28). Several studies have also shown that nonsteroidal anti-inflammatory drugs (NSAIDs) increase the mucosal permeability (15).…”

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confidence: 99%

Dietary nitrate increases gastric mucosal blood flow and mucosal defense

Petersson

Phillipson²,

Jansson³

et al. 2007

American Journal of Physiology-Gastrointestinal and Liver Physi

130

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Salivary nitrate from dietary or endogenous sources is reduced to nitrite by oral bacteria. In the acidic stomach, nitrite is further reduced to bioactive nitrogen oxides, including nitric oxide (NO). In this study, we investigated the gastroprotective role of nitrate intake and of luminally applied nitrite against provocation with diclofenac and taurocholate. Mucosal permeability ( 51 Cr-EDTA clearance) and gastric mucosal blood flow (laser-Doppler flowmetry) were measured in anesthetized rats, either pretreated with nitrate in the drinking water or given acidified nitrite luminally. Diclofenac was given intravenously and taurocholate luminally to challenge the gastric mucosa. Luminal NO content and nitrite content in the gastric mucus were determined by chemiluminescence. The effect of luminal administration of acidified nitrite on the mucosal blood flow was also investigated in endothelial nitric oxide synthasedeficient mice. Rats pretreated with nitrate or given nitrite luminally had higher gastric mucosal blood flow than controls. Permeability increased more during the provocation in the controls than in the nitrate-and nitrite-treated animals. Dietary nitrate increased luminal NO levels 50 times compared with controls. Nitrate intake also resulted in nitrite accumulation in the loosely adherent mucous layer; after removal of this mucous layer, blood flow was reduced. Nitrite administrated luminally in endothelial nitric oxide synthase-deficient mice increased mucosal blood flow. We conclude that dietary nitrate and direct luminal application of acidified nitrite decrease diclofenacand taurocholate-induced mucosal damage. The gastroprotective effect likely involves a higher mucosal blood flow caused by nonenzymatic NO production. These data suggest an important physiological role of nitrate in the diet. laser-Doppler flowmetry;51 chromium-labeled EDTA clearance; nitrite; rats; endothelial nitric oxide synthase deficient; gastric mucus THE ROLE OF NITRATE IN OUR diet has been a subject of great controversy over the years. The anxiety concerning nitrate consumption is related to the presumed association with gastrointestinal cancer. Today, however, there are more studies contradicting than supporting such a correlation (36). Several recent studies have in fact suggested that dietary nitrate has a gastroprotective role (6,16,31,32,35,38). Most of the nitrate in our diet originates from green leafy vegetables, a natural source of food for humans. Bacteria in the oral cavity reduce parts of the nitrate to nitrite, and human saliva therefore contains both of these anions (49). A large amount of nitric oxide (NO) is produced in the upper gut when swallowed nitrite reacts with hydrogen ions (6,35,37).

show abstract

Nitric oxide prevents rat duodenal contractions induced by potentially noxious agents

Cited by 6 publications

References 26 publications

Effects of Capsaicin Supplementation on Productive and Physiological Performance of Pekin Ducks During Summer Season

Effects of Capsaicin Supplementation on Productive and Physiological Performance of Pekin Ducks During Summer Season

Melatonin decreases duodenal epithelial paracellular permeability via a nicotinic receptor–dependent pathway in rats in vivo

Dietary nitrate increases gastric mucosal blood flow and mucosal defense

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