Nitric oxide synthase in the glossopharyngeal and vagal afferent pathway of a teleost, Takifugu niphobles

Funakoshi, Kengo; Kadota, Tetsuo; Atobe, Yoshitoshi; Nakano, Masato; Goris, Richard C.; Kishida, Reiji

doi:10.1007/s004419900078

Cited by 29 publications

(18 citation statements)

References 43 publications

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“…Our positive results, together with previous histochemical results in other nonmammalian vertebrates including teleosts (Holmqvist et al, 1994;Holmqvist and Ekström, 1997;Olsson and Holmgren, 1997;Karila and Holmgren, 1997;Funakoshi et al, 1999), suggest that NOS is a highly conserved enzyme amongst vertebrates. Several studies have established the presence of ntype NOS and i-type NOS, but not eNOS, in teleost fish (e.g.…”

Section: Discussionsupporting

confidence: 82%

Nitric oxide in control of luminescence from hatchetfish(Argyropelecus hemigymnus) photophores

Krönström

Holmgren

Baguet

et al. 2005

Journal of Experimental Biology

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Incubation of photophores in the NO donors sodium nitroprusside or S-nitroso-N-acetylpenicillamine prior to adrenaline stimulation reduced the intensity of the strong and long-lasting type of response, but had little or even a potentiating effect on the weakly responding photophores. Hydroxylamine, which is converted to NO if catalase activity is present in the tissue, reduced the duration and the intensity of the adrenaline response in all tested organs. The NOS-inhibitor L-thiocitrulline potentiated the adrenaline response in the weakly responding organs; the weaker the adrenaline effect, the stronger the potentiation caused by L-thiocitrulline. The strongly responding organs were instead inhibited by L-thiocitrulline. The results suggest that NO has an important role in the control of light emission from Argyropelecus hemigymnus photophores. The cGMP analogue dibutyryl cGMP, the guanylate cyclase inhibitor ODQ and the phosphodiesterase inhibitor pentoxiphylline had no effect, indicating that the NO effect does not involve cGMP.

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Section: Discussionsupporting

confidence: 82%

Nitric oxide in control of luminescence from hatchetfish(Argyropelecus hemigymnus) photophores

Krönström

Holmgren

Baguet

et al. 2005

Journal of Experimental Biology

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“…Histochemistry also demonstrated that NADPH-diaphorase activity (indicating the presence of nitric oxide synthase) is present in the resident neurons of fish gill (Gibbins et al, 1995), while immunohistochemistry revealed a nitrergic vagal sensory innervation of the gill vasculature in the pufferfish, Takifugu niphobles (Funakoshi et al, 1999) and our studies demonstrated that most arteries of the gill and air sac of H. fossilis share a NANC (basically nitrergic) innervation (Zaccone et al, 2003a, b). In contrast, there is no information on the innervation of the accessory air-breathing organs of teleosts and the lung of polypterids.…”

Section: Introductionsupporting

confidence: 53%

Neurochemical features of the innervation of respiratory organs in some air‐breathing fishes

Mauceri¹,

Fasulo

Minniti

et al. 2005

Italian Journal of Zoology

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A number of studies has been performed in recent years regarding the structure of the autonomic innervation of the gill and various organs of fishes, but only limited information exists on the role of the autonomic nervous system in air-breathing organs. The mechanisms for physiologically integrating gill and air-breathing organs are also not fully understood. The presence of neuropeptides and nNOS in gill autonomie nerves has been investigated in recent years by several Authors. Immunohistochemical localization of neuropeptides and nNOS in the autonomie nerves in the vasculatures and smooth muscle of air-breathing organs is reported for the first time. The function of NO in these tissues also remains to be elucidated especially, when the bulk of neurotransmission is attributable to cholinergic and adrenergic mechanisms. A challenge for future years is to understand the role of the autonomie nervous systems and the vasoactive substances in the in-series and in-parallel vascular connections between systemic gill and air-breathing organs.

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“…Previously, the localization of NOS and/or NADPHd in the gills of fish has only been reported in the nitrergic nerve fibers coursing along the efferent branchial artery in the puffer fish Takifugu niphobles (Funakoshi et al, 1999). Also, NADPHd activity has been localized to parasympathetic postganglionic neurons in branchial nerves nIX and nX in the cod Gadus morhua (Gibbins et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide synthase in the gill of Atlantic salmon: colocalization with and inhibition of Na+,K+-ATPase

Ebbesson

Tipsmark

Holmqvist

et al. 2005

Journal of Experimental Biology

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SUMMARY We investigated the relationship between nitric oxide (NO) and Na+,K+-ATPase (NKA) in the gill of anadromous Atlantic salmon. Cells containing NO-producing enzymes were revealed by means of nitric oxide synthase (NOS) immunocytochemistry and nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochemistry, which can be used as an indicator of NOS activity, i.e. NO production. Antibodies against the two constitutive NOS isoforms, neuronal and endothelial NOS, both produced immunoreactivity restricted to large cells at the base and along the secondary lamellae. NADPHd-positive cells showed a corresponding distribution. Antibodies against the inducible NOS isoform only labeled small cells located deep in the filament. Using in situ hybridization and NKA immunoreactivity, cells expressing Na+,K+-ATPaseα-subunit mRNA were found to have a similar distribution to the NOS cells. Double labeling for NOS immunoreactivity and NKA α-subunit mRNA revealed cellular colocalization of NKA α-subunit mRNA and nNOS protein in putative chloride cells at the base of the lamellae and interlamellar space. Along the lamellae, some NOS- or NKA-immunoreactive cells possessed a relatively lower expression of NKA α-subunit mRNA in smolts. A clear increase in NADPHd staining in the gill was demonstrated from parr to smolt. The regulatory role of NO on gill NKA activity was studied in vitrousing sodium nitroprusside (SNP; 1 mmol l-1) and PAPA-NONOate(NOC-15; 0.5 mmol l-1) as NO donors. Both SNP and NOC-15 inhibited gill NKA activity by 30% when compared to controls. The study shows that NO systems are abundant in the gill of Atlantic salmon, that NO may be produced preferentially by a constitutive NOS isoform, and suggests that NO influence on gill functions is mediated via intracellular, possibly both auto/paracrine,inhibition of Na+,K+-ATPase activity in chloride cells. Furthermore, the increase in NADPHd in the gill during smoltification suggests a regulatory role of NO in the attenuation of the smoltification-related increase in Na+,K+-ATPase activity prior to entering seawater.

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Nitric oxide synthase in the glossopharyngeal and vagal afferent pathway of a teleost, Takifugu niphobles

Cited by 29 publications

References 43 publications

Nitric oxide in control of luminescence from hatchetfish(Argyropelecus hemigymnus) photophores

Nitric oxide in control of luminescence from hatchetfish(Argyropelecus hemigymnus) photophores

Neurochemical features of the innervation of respiratory organs in some air‐breathing fishes

Nitric oxide synthase in the gill of Atlantic salmon: colocalization with and inhibition of Na+,K+-ATPase

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