Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: Molecular cloning and expression

Scheinker, Vladimir; Fiore, Gabriella; Cristo, Carlo Di; Cosmo, Anna Di; d’Ischia, Marco; Enikolopov, Grigori; Palumbo, Anna

doi:10.1016/j.bbrc.2005.10.069

Cited by 35 publications

(39 citation statements)

References 47 publications

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“…In spite of the large number of studies concerning NO production, the NOS gene has been identified in only a few species of invertebrates including insects, two shrimp species [33,34], one snail [35], and one mollusk [36]. A NOS-like gene in the oyster Crassostrea virginica has been isolated [37] but the gene in R. philippinarum has not yet been identified.…”

Section: Discussionmentioning

confidence: 96%

Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

Jeffroy

Paillard

2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 96%

Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

Jeffroy

Paillard

2011

Fish & Shellfish Immunology

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“…Through this simple histochemical reaction, NOS-like enzymes have been identified in the neurons of a number of vertebrate and invertebrate taxa and NO was recognized as one of the most ancient signaling molecules which is widespread in all animal phyla (see González-Domenech and Muñoz-Chápuli, 2010;Andreakis et al, 2011, for phylogenetic analyses of metazoan NOS). The molecular characteristics of invertebrate NOSs have been identified in some taxa: cnidarians (Panchin et al, 2002), arthropods (Regulski and Tully, 1995;Yuda et al, 1996;Nighorn et al, 1998;Imamura et al, 2002;Watanabe et al, 2005;Takahashi et al, 2009), molluscs (Scheinker et al, 2005), and amphioxus (Panchin et al, 2003). Most invertebrate NOSs appear to be similar to the constitutive neuronal NOS of mammals, except for their N-termini, which lack a PSD-95/discs-large/ZO-1 (PDZ) domain.…”

Section: Indexing Terms: Nos; Stramonita Haemastoma; Nadph Diaphorasementioning

confidence: 97%

Cellular, biochemical, and molecular characterization of nitric oxide synthase expressed in the nervous system of the prosobranch Stramonita haemastoma (Gastropoda, Neogastropoda)

Cioni¹,

Patti²,

Venturini³

et al. 2011

J of Comparative Neurology

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Nitric oxide synthase (NOS) has been characterized in several opistobranchs and pulmonates but it was much less investigated in prosobranchs, which include more than 20,000 species and account for most of the gastropod diversity. Therefore, new data from this large group are needed for a better knowledge of the molecular evolution of NOS enzymes in molluscs. This study focused on NOS expressed in the nervous system of the prosobranch neogastropod Stramonita haemastoma. In this study we report compelling evidence on the expression of a constitutive Ca(2+) /CaM-dependent neuronal NOS in the central and peripheral nervous system. The prevailing neuronal localization of NADPHd activity was demonstrated by NADPHd histochemistry in both central and peripheral nervous system structures. L-arginine/citrulline assays suggested that Stramonita NOS is a constitutive enzyme which is both cytosolic and membrane-bound. Molecular cloning of the full-length Stramonita NOS (Sh-NOS) by reverse-transcription polymerase chain reaction (RT-PCR) followed by 5' and 3' RACE showed that Sh-NOS is a protein of 1,517 amino acids, containing a PDZ domain at the N-terminus and sharing similar regulatory domains to the mammalian neuronal NOS (nNOS). Regional expression of the Sh-NOS gene was evaluated by RT-PCR. This analysis showed different expression levels in the nerve ring, the osphradium, the cephalic tentacles, the buccal tissues, and the foot, whereas NOS expression was not found in the salivary glands and the gland of Leiblein. The present data provide a solid background for further studies addressing the specific functions of NO in neogastropods.

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“…In mollusks, NOS-like activity has been detected in several species of bivalves, gastropods and cephalopods (reviewed in Palumbo, 2005), and NOS-encoding genes have been isolated from the gastropods Aplysia californica and Lymnaea stagnalis and the cephalopod Sepia officinalis (Korneev et al, 1997;Korneev and O'Shea, 2002;Scheinker et al, 2005). Nitric oxide was shown to be involved in neuromodulation in the central nervous system (Gelperin et al, 2000;Zsombok et al, 2000;Inoue et al, 2001), metamorphosis (Hens et al, 2006) and immune defense of mollusks (Tafalla et al, 2002;Tafalla et al, 2003;Novas et al, 2004;Villamil et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of cadmium exposure and intermittent anoxia on nitric oxide metabolism in eastern oysters,Crassostrea virginica

Ivanina

Eilers

Kurochkin

et al. 2010

Journal of Experimental Biology

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SUMMARY Nitric oxide (NO) is an intracellular signaling molecule synthesized by a group of enzymes called nitric oxide synthases (NOS)and involved in regulation of many cellular functions including mitochondrial metabolism and bioenergetics. In invertebrates, the involvement of NO in bioenergetics and metabolic responses to environmental stress is poorly understood. We determined sensitivity of mitochondrial and cellular respiration to NO and the effects of cadmium (Cd) and intermittent anoxia on NO metabolism in eastern oysters, Crassostrea virginica. NOS activity was strongly suppressed by exposure to 50gl -1 Cd for 30days (4.76 vs 1.19pmol NO min -1 mg -1 protein in control and Cd-exposed oysters, respectively) and further decreased during anoxic exposure in Cd-exposed oysters but not in their control counterparts. Nitrate/nitrite content (indicative of NO levels) decreased during anoxic exposure to less than 10% of the normoxic values and recovered within 1h of re-oxygenation in control oysters. In Cd-exposed oysters, the recovery of the normoxic NO levels lagged behind, reflecting their lower NOS activity. Oyster mitochondrial respiration was inhibited by exogenous NO, with sensitivity on a par with that of mammalian mitochondria, and ADP-stimulated mitochondrial respiration was significantly more sensitive to NO than resting respiration. In isolated gill cells, manipulations of endogenous NOS activity either with a specific NOS inhibitor (aminoguanidine) or a NOS substrate (L-arginine) had no effect on respiration, likely due to the fact that mitochondria in the resting state are relatively NO insensitive. Likewise, Cdinduced stimulation of cellular respiration did not correlate with decreased NOS activity in isolated gill cells. High sensitivity of phosphorylating (ADP-stimulated) oyster mitochondria to NO suggests that regulation of bioenergetics is an evolutionarily conserved function of NO and that NO-dependent regulation of metabolism may be most prominent under the conditions of high metabolic flux when the ADP-to-ATP ratio is high. Supplementary material available online at

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Nitric oxide synthase in the nervous system and ink gland of the cuttlefish Sepia officinalis: Molecular cloning and expression

Cited by 35 publications

References 47 publications

Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

Cellular, biochemical, and molecular characterization of nitric oxide synthase expressed in the nervous system of the prosobranch Stramonita haemastoma (Gastropoda, Neogastropoda)

Effects of cadmium exposure and intermittent anoxia on nitric oxide metabolism in eastern oysters,Crassostrea virginica

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