Nitrification performance of nitrifiers immobilized in PVA (polyvinyl alcohol) for a marine recirculating aquarium system

Seo, Jae-Koan; Jung, Il-Hyong; Kim, Mi-Ryung; Kim, Byong Jin; Nam, Soo-Wan; Kim, Sung-Koo

doi:10.1016/s0144-8609(01)00063-2

Cited by 55 publications

(30 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Labomascus & Robinson (1988) and Piedrahita (2005) suggested that a fast way to activate a biofilter is through an inoculation generation process or bacterial seed. Therefore, an active-immobilized nitrifying inoculator was generated inside a 10 L continuous airlift bioreactor (Seo et al, 2001;Gutierrez, 2008). Same quantity of available surface area of each media.…”

Section: Constitution Of Re-conditioned Freshwatermentioning

confidence: 99%

Nitrogenated compounds biofiltration under alternative bacterium fixation substrates

Carroza¹,

Hurtado²,

Gutierrez³

2012

lajar

View full text Add to dashboard Cite

ABSTRACT. This study compares the behavior of nitrification (NH 4 + , NO 2 -and NO 3 -), and performance, in terms of the surface TAN conversion rate (STR), volumetric TAN conversion rate (VTR) and removal percentage of TAN (PTR) among three fixation media of nitrifying bacteria (two alternatives (S 1 , S 2 ) and one commercial (C o )). The experiment was performed in two tests of 42 days each. Three isolated biofiltration systems were built for the experience, to which were added media colonized by bacteria as a "seed" to start the process of nitrification. Ammonium chloride (NH 4 Cl) was attached as source of ammonium in reconditioned freshwater, also gradually adding inorganic carbon (HCO 3 -) to maintain moderate water hardness. ), y el rendimiento, en términos de la tasa superficial de conversión de NAT, tasa volumétrica de conversión de NAT y porcentaje de remoción de NAT (PRN) entre tres medios de fijación de bacterias nitrificantes, dos alternativos (S 1 , S 2 ) y uno comercial (Co). La experiencia se realizó en dos pruebas de 42 días cada una. Se construyeron tres sistemas aislados para la experiencia, a los cuales se adicionaron medios colonizados por bacterias a modo de "semilla", para el inicio del proceso de nitrificación. Cloruro de amonio (NH 4 Cl) fue adherido como fuente de amonio en agua dulce re acondicionada, adicionando gradualmente también carbón inorgánico (HCO 3 -) para mantener una dureza moderada del agua. Los resultados promedio para ambas pruebas, indican que los sustratos S 1 y S 2 muestran un comportamiento estadísticamente similar al sustrato C o (P > 0,05) durante los primeros 33 días (hasta el estado estacionario). Para el segundo test, en términos de rendimiento de STR se registraron valores de 0,4, 0,39, y 0,39 g TAN m -2 d -1 para S 1 , S 2 y C o , respectivamente; en términos de PRN valores de 92, 97, 93% para S 1 , S 2 y C o , respectivamente y en función de VTR se registraron valores de 72,31, 114,94, 39,02 g NAT m -3 d -1 , para S 1 , S 2 y C o , respectivamente. Los análisis estadísticos aportaron que, para STR y PRN, no se encontraron diferencias significativas. En cambio, para VTR se identificaron diferencias estadísticamente significativas entre los medios evaluados, siendo el medio S 2 , el que presenta un mayor valor de VTR.

show abstract

Section: Constitution Of Re-conditioned Freshwatermentioning

confidence: 99%

Nitrogenated compounds biofiltration under alternative bacterium fixation substrates

Carroza¹,

Hurtado²,

Gutierrez³

2012

lajar

View full text Add to dashboard Cite

show abstract

“…The most prominent characteristic of any RAS is a nitrifying biofilter to prevent accumulation of metabolites like ammonia and nitrite; which at high levels undermine the commercial production as their toxic impacts are manifested through impaired growth or chronic diseases (Tomasso, 1994;Cheng et al, 2004;Sovobodova et al, 2005). Fixed film biofilters are commonly used for total ammonia nitrogen (TAN) removal in RAS, where attached growth as biofilm offers several advantages such as handling convenience, increased process stability to shock loading and prevention of the bacterial population from being washed off (Fitch et al, 1998;Seo et al, 2001;Shnel et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the nitrifying bacterial consortia employed for the activation of bioreactors used in brackish and marine aquaculture systems

Kumar

Sukumaran

Achuthan

et al. 2013

International Biodeterioration & Biodegradation

View full text Add to dashboard Cite

a b s t r a c tThe addition of commercial nitrifying bacterial products has resulted in significant improvement of nitrification efficiency in recirculating aquaculture systems (RAS). We developed two nitrifying bacterial consortia (NBC) from marine and brackish water as start up cultures for immobilizing commercialized nitrifying bioreactors for RAS. In the present study, the community compositions of the NBC were analyzed by universal 16S rRNA gene and bacterial amoA gene sequencing and fluorescence in situ hybridization (FISH). This study demonstrated that both the consortia involved autotrophic nitrifiers, denitrifiers as well as heterotrophs. Abundant taxa of the brackish water heterotrophic bacterial isolates were Paenibacillus and Beijerinckia spp. whereas in the marine consortia they were Flavobacterium, Cytophaga and Gramella species. The bacterial amoA clones were clustered together with high similarity to Nitrosomonas sp. and uncultured beta Proteobacteria. FISH analysis detected ammonia oxidizers belonging to b subclass of proteobacteria and Nitrosospira sp. in both the consortia, and Nitrosococcus mobilis lineage only in the brackish water consortium and the halophilic Nitrosomonas sp. only in the marine consortium. However, nitrite oxidizers, Nitrobacter sp. and phylum Nitrospira were detected in both the consortia. The metabolites from nitrifiers might have been used by heterotrophs as carbon and energy sources making the consortia a stable biofilm.

show abstract

“…This is especially true in shrimp/prawn hatcheries where the daily specific excretion of ammonia by larvae and post-larvae is five fold higher than that of adults. To address this issue, fixed film biofilters are commonly employed for total ammonia nitrogen (TAN) removal (Seo et al 2001;Shnel et al 2002). Experience has shown that the biofilters in RAS require activation to achieve high bacterial growth (Valenti and Daniels 2000) due to the habitually low concentrations of nutrients in the system (Piedrahita 2003), where natural bacterial yield is too low, thus delaying colonization and biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Mass production of nitrifying bacterial consortia for the rapid establishment of nitrification in saline recirculating aquaculture systems

Kumar

Achuthan

Manju

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Two distinct nitrifying bacterial consortia, namely an ammonia oxidizing non-penaeid culture (AMO NPCU-1) and an ammonia oxidizing penaeid culture (AMOPCU-1), have been mass produced in a nitrifying bacterial consortia production unit (NBCPU). The consortia, maintained at 4°C were activated and cultured in a 2 l fermentor initially. At this stage the net biomass (0.105 and 0.112 g/l), maximum specific growth rate (0.112 and 0.105/h) and yield coefficients (1.315 and 2.08) were calculated respectively, for AMONPCU-1 and AMOPCU-1 on attaining stationary growth phase. Subsequently on mass production in a 200 l NBCPU under optimized culture conditions, the total amounts of NH 4 ? -N removed by AMONPCU-1 and AMOPCU-1 were 1.948 and 1.242 g/l within 160 and 270 days, respectively. Total alkalinity reduction of 11.7-14.4 and 7.5-9.1 g/l were observed which led to the consumption of 78 and 62 g Na 2 CO 3 . The yield coefficient and biomass of AMONPCU-1 were 0.67 and 125.3 g/l and those of AMOPCU-1 were 1.23 and 165 g/l. The higher yield coefficient and growth rate of AMOPCU-1 suggest better energy conversion efficiency and higher CO 2 fixation potential. Both of the consortia were dominated by Nitrosomonas-like organisms. The consortia may find application in the establishment of nitrification within marine and brackish water culture systems.

show abstract

Nitrification performance of nitrifiers immobilized in PVA (polyvinyl alcohol) for a marine recirculating aquarium system

Cited by 55 publications

References 12 publications

Nitrogenated compounds biofiltration under alternative bacterium fixation substrates

Nitrogenated compounds biofiltration under alternative bacterium fixation substrates

Molecular characterization of the nitrifying bacterial consortia employed for the activation of bioreactors used in brackish and marine aquaculture systems

Mass production of nitrifying bacterial consortia for the rapid establishment of nitrification in saline recirculating aquaculture systems

Contact Info

Product

Resources

About