Nitrite induces endoplasmic reticulum stress and associates apoptosis of liver cells in grass carp (Ctenopharyngodon idella)

Xie, Lixia; Chen, Siqi; Yao, Chaorui; Li, Dapeng; Li, Li; Tang, Rong

doi:10.1016/j.aquaculture.2019.04.016

Cited by 35 publications

(9 citation statements)

References 59 publications

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“…(Boyd & Tucker, 2019; Cudmore et al, 2004; Djiba et al, 2021; Goddard, 1996). Although C. idella is a rheophilic species, known for its great tolerance and adaptability to different environmental conditions, its welfare in breeding is inextricably linked to relatively well‐established water quality conditions (Cudmore et al, 2004; Ijaz et al, 2010; Xie et al, 2019; Zhao et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

New indices for the diagnosis of fish welfare and their application to the grass carp ( Ctenopharyngodon idella ) reared in earthen ponds

Pedrazzani¹,

Quintiliano²,

Cozer

et al. 2022

Aquaculture Research

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Animal welfare is an issue of increasing importance in global aquaculture. However, the incorporation of this issue into routine aquaculture operations is a challenge. A possible path to change this is the recognition, identification and addressing of the ecological, physiological, nutritional, behavioural and psychological needs of fish. The present study uses grass carp (Ctenopharyngodon idella), the world's most widely produced fish species, and earth ponds, the world's most widely used production system in fish farming worldwide, as the basis for developing indices to assess and monitor the welfare of farmed fish. The proposed indices were structured based on specific welfare indicators, reference values, individual weights and associated scores to address the Animal Welfare Five Freedoms and a Life Worth living concepts. Two types of indices were proposed: (1) partial welfare indices (PWIx), specific to each of the four observed/analysed freedoms; (2) general welfare index (GWI), which simultaneously summarizes the addressees' freedoms in a single variable. Both indices range from 0 (critical welfare impairment) to 1.0 (minimal risk of welfare impairment). The study was based on a comprehensive systematic review of the literature using the PRISMA method. The proposed indices were based on 10 environmental indicators, nine indicators for health, five for nutrition and four for behaviour. The PWIx can be used to determine how each category of indicators contributes to the GWI, which defines the level of fish farm welfare at a given point in time and which needs are affected or met during a production cycle.

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Section: Discussionmentioning

confidence: 99%

New indices for the diagnosis of fish welfare and their application to the grass carp ( Ctenopharyngodon idella ) reared in earthen ponds

Pedrazzani¹,

Quintiliano²,

Cozer

et al. 2022

Aquaculture Research

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“…Furthermore, the endoplasmic reticulum (ER), as one of the most important organelles in eukaryotic cells, is closely related to cell homeostasis, and ER homeostasis is maintained through unfolded protein reactions involving three signalling pathways: the PEPK-signalling pathway, IRE-1-signalling pathway, and ATF6-signalling pathway [ 16 ]. In aquatic animals, numerous studies have reported that adverse factors could induce endoplasmic-reticulum stress (ERS) by affecting the mRNA levels of core genes in the IRE1-signalling pathway, PERK-signalling pathway, and ATF6-signalling pathway, such as high-fat induction in blunt snout bream ( Megalobrama amblycephala ) [ 17 ], hydrogen peroxide exposure and ammonia stress in common carp ( Cyprinus carpio ) [ 18 , 19 ], waterborne cadmium exposure in Gibel carp ( Carassius gibelio ) [ 20 ], and nitrite induction in grass carp ( Ctenopharyngodon idella ) [ 21 ]. When ERS occurs or is persistent and exceeds the range of cellular self-regulation, it leads to cell apoptosis and death and induces disease [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Histidine Deficiency Inhibits Intestinal Antioxidant Capacity and Induces Intestinal Endoplasmic-Reticulum Stress, Inflammatory Response, Apoptosis, and Necroptosis in Largemouth Bass (Micropterus salmoides)

Liang

Gao

et al. 2022

Antioxidants

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This 56-day study aimed to evaluate the effects of histidine levels on intestinal antioxidant capacity and endoplasmic-reticulum stress (ERS) in largemouth bass (Micropterus salmoides). The initial weights of the largemouth bass were (12.33 ± 0.01) g. They were fed six graded levels of histidine: 0.71% (deficient group), 0.89%, 1.08%, 1.26%, 1.48%, and 1.67%. The results showed that histidine deficiency significantly suppressed the intestinal antioxidant enzyme activities, including SOD, CAT, GPx, and intestinal level of GSH, which was supported by significantly higher levels of intestinal MDA. Moreover, histidine deficiency significantly lowered the mRNA level of nrf2 and upregulated the mRNA level of keap1, which further lowered the mRNA levels of the downstream genes sod, cat, and gpx. Additionally, histidine-deficiency-induced intestinal ERS, which was characterized by activating the PEPK-signalling pathway and IRE1-signalling pathway, including increased core gene expression of pepk, grp78, eif2α, atf4, chopα, ire1, xbp1, traf2, ask1, and jnk1. Dietary histidine deficiency also induced apoptosis and necroptosis in the intestine by upregulating the expressions of proapoptotic genes, including caspase 3, caspase 8, caspase 9, and bax, and necroptosis-related genes, including mlkl and ripk3, while also lowering the mRNA level of the antiapoptotic gene bcl-2. Furthermore, histidine deficiency activated the NF-κB-signalling pathway to induce an inflammatory response, improving the mRNA levels of the proinflammatory factors tnf-α, hepcidin 1, cox2, cd80, and cd83 and lowering the mRNA levels of the anti-inflammatory factors tgf-β1 and ikbα. Similarly, dietary histidine deficiency significantly lowered the intestinal levels of the anti-inflammatory factors TGF-β and IL-10 and upregulated the intestinal levels of the proinflammatory factor TNF-α, showing a trend similar to the gene expression of inflammatory factors. However, dietary histidine deficiency inhibited only the level of C3, and no significant effects were observed for IgM, IgG, HSP70, or IFN-γ. Based on the MDA and T-SOD results, the appropriate dietary histidine requirements of juvenile largemouth bass were 1.32% of the diet (2.81% dietary protein) and 1.47% of the diet (3.13% dietary protein), respectively, as determined by quadratic regression analysis.

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“…High concentrations of nitrite can cause nitrite accumulation in blood plasma (Thi et al, 2019). Also, the nitrite can interfere with the physiological function like oxygen capacity of cell hemoglobin, immune system health, physiological metabolism, and endocrine regulation (Xie et al, 2019). Fishes have various mechanisms to detoxify nitrites, such as methemoglobin reductase secretion or nitrite to nitrate oxidation as this compound is less toxic (Kim et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen excretion and oxygen consumption on hyper intensive tilapia Oreochromis niloticus culture using three different commercial diets

López-Tejeida

García‐Trejo²,

Félix-Cuencas³

et al. 2020

Lat. Am. J. Aquat. Res.

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The fish feed used in aquaculture systems has many nutritional factors like protein, fat, fiber, ash, and humidity; from these factors, one of great importance is protein. The protein of fish feed is obtained from fish meal, and the demand for fish meal has increased; this induces to increase the price. Many pieces of research have been done about this topic to mitigate this problem. This research shows the differences between three commercial foods of the different processes with the same quantity of protein, using tilapia Oreochromis niloticus, to prove any difference between NO2-, NO3-, and NH4+. Nitrogen compounds were determined with HACH methods for the DR6000 spectrophotometer. Biometric data were registered throughout the growth of fish, along with measure aerobic metabolism. Results have shown that has better outcomes related to low excretion of nitrogen compound was medium-cost fish feed (MCFF). It also has sound effects associated with the length-weight relationship; that could be connected to these fish feed's protein sources.

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Nitrite induces endoplasmic reticulum stress and associates apoptosis of liver cells in grass carp (Ctenopharyngodon idella)

Cited by 35 publications

References 59 publications

New indices for the diagnosis of fish welfare and their application to the grass carp ( Ctenopharyngodon idella ) reared in earthen ponds

New indices for the diagnosis of fish welfare and their application to the grass carp ( Ctenopharyngodon idella ) reared in earthen ponds

Histidine Deficiency Inhibits Intestinal Antioxidant Capacity and Induces Intestinal Endoplasmic-Reticulum Stress, Inflammatory Response, Apoptosis, and Necroptosis in Largemouth Bass (Micropterus salmoides)

Nitrogen excretion and oxygen consumption on hyper intensive tilapia Oreochromis niloticus culture using three different commercial diets

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