Nitro-Substituted Ruthenium(II) Complex: A New Strategy for a G-Quadruplex DNA Fluorescent Probe

Deng

et al. 2020

Applied Organom Chemis

Self Cite

The detection of G-quadruplex is of major interest. Nitro-substituted ruthenium (II) complexes have attracted much attention due to fluorescent sensitivity to environment change. We report here a new nitro-substituted ruthenium (II) complex, [Ru (phen) 2 (hnoip)] 2+ (1) (hnoip = 2-(2-hydroxyl-5-nitrophenyl) imidazo[4,5-f][1,10-phenanthroline]), which displays distinct fluorescent properties in aqueous solution and non-aqueous solvents. This complex exhibits large fluorescence enhancement after binding with G-quadruplex DNA, and displays good fluorescent selectivity over other DNAs. The limit of detection is 6 nM for 22AG in Na + and 43 nM for 22AG in K + , respectively. The results demonstrated that nitro-substituted ruthenium (II) complexes can be utilized to design as G-quadruplex fluorescent probes by protection of the nitro group on the complex from water.

Section: Fluorescence Spectra Of Complex 1 In Various Solventsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An unexpected fluorescent probe for G‐quadruplex DNA based on a nitro‐substituted ruthenium (II) complex

Deng

et al. 2020

Applied Organom Chemis

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“…Luminescent transition metal complexes, especially ruthenium (II) (Ru(II)) and iridium (III) (Ir(III)) complexes, have received considerable attention in the development of bioassay and imaging probes [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

A Ruthenium(II) complex-based probe for colorimetric and luminescent detection and imaging of hydrogen sulfide in living cells and organisms

Zhang

et al. 2021

Analytica Chimica Acta

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A luminescence probe for c‐myc G‐quadruplex by a triphenylamine‐appended ruthenium complex

Deng

et al. 2021

Applied Organom Chemis

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Due to the essential roles of G‐quadruplex in biological processes, the development of G‐quadruplex luminescent probe is becoming more and more important for further understanding of the functions of G‐quadruplex in biology. We herein reported that a ruthenium complex containing triphenylamine group exhibited “off–on” emission behavior after binding to G‐quadruplex in the presence of luminescence quencher [Fe (CN6)]4−. The Ru‐Fe system consists of a mixture of [Ru (phen)2(TPAD)]2+, where TPAD = N‐4‐(1H‐imidazo [4,5‐f] [1,10]phenanthroline‐2‐yl phenyl)‐N‐phenyl‐benzenamine and [Fe (CN6)]4−, which showed luminescent selectivity toward parallel G‐quadruplex (c‐myc). The detection limit was 159 nM for c‐myc. The ligand TPAD bearing nonplanar triphenylamine group and steric hindrance led to different DNA affinities toward various DNA structures. Luminescence experiments, CD spectra, G4‐FID, FRET (fluorescence resonance energy transfer) measurements, and molecular docking results indicated that different DNA affinities and binding modes of the complex toward various DNA structures result in the luminescence selectivity.