Nitroblue-tetrazolium test for the functional evaluation of phagocytic cells: A critical analysis of the methodology

Müller, J.; Alföldy, P; Lemmel, E.-M.

doi:10.1007/bf01982475

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…2.7. Respiratory burst NBT reduction test was performed as described previously with some modifications (Müller et al, 1981;Nabi et al, 2005;Hamaliaka and Novikova, 2010). In brief, 200 µL of neutrophil suspension (25 × 10 5 cell/mL) was mixed with 200 µL/mL of S. aureus suspension (10 8 cells/mL) and 200 µL of 0.1% NBT in PBS (pH 7.4).…”

Section: Phagocytosis Assaymentioning

confidence: 99%

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

Galeh¹,

Delirezh²,

Froushani³

et al. 2014

Turk J Biol

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IntroductionComplicated crosstalk between environmental factors and multiple genes determines which individuals will develop any given immune-mediated disease (Cantorna, 2010). Calcitriol [1α-25(OH)-vitamin D3] is one of the steroid hormone families and, similar to other members of these families, participates in the regulation of gene expression (Cantorna, 2010;Smyk et al., 2013). On the other hand, calcitriol may be an environmental factor that contributes to immune-mediated disease development. Environmental sources of calcitriol include diet and production in the skin following UV exposure to precursor 7-dehydrocholesterol (Namgung et al., 1994).Bone-marrow-derived mesenchymal stem cells (MSCs) are multipotent and can give rise to mesenchymal tissues like bone, cartilage, and fat (Uccelli et al., 2008). They also have potent immunomodulatory properties and may be valuable tools for cell-based immunotherapy (Meirelles Lda et al., 2009;Ghannam et al., 2010;Zhang et al., 2013). MSCs in bone marrow and tissue form a niche that has inevitable interactions with hematopoietic cells including neutrophils (Raffaghello et al., 2008;Maqbool et al., 2011). Neutrophils are one of the major cell types that constitute innate immunity. They predominate in host tissues during acute inflammatory processes (Greenberg and Grinstein, 2002).Recent documents have shown that calcitriol has an important role in regulating the growth of MSCs (Artaza et al., 2010;Klotz et al., 2012). The present study was carried out to investigate the effects of calcitriol on the interaction between bone-marrow-derived MSCs and neutrophils in rats. Materials and methods MaterialsPropidium iodide, acridine orange, and phosphatebuffered saline (PBS) were procured from Sigma-Aldrich (St Louis, MO, USA). May-Grünwald-Giemsa stain was purchased from Merck (Darmstadt, Germany) and dextran was obtained from Fresenius Kabi (Verona, Italy). Fetal calf serum, Dulbecco's Modified Eagle Medium (DEMEM), and RPMI 1640 were purchased from GIBCO/Life Technologies Inc. (Gaithersburg, MD, USA). The enzymelinked immunosorbent assay (ELISA) kit for interleukin (IL)-6 was purchased from Bender MedSystems

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Section: Phagocytosis Assaymentioning

confidence: 99%

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

Galeh¹,

Delirezh²,

Froushani³

et al. 2014

Turk J Biol

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“…In the case of phagocyte cells, it is linked to phagocytosis. The reduction of NBT may also be linked to the oxidative basal state of the cell (22) as well as in chronic granulomatous disease, where there is an oxidative metabolism alteration in phagocytes (23). This test can also measure cell adhesion, lysosomal degranulation (24) and even the oxidative capacity of isolated mitochondria (25).…”

Section: Discussionmentioning

confidence: 99%

Increased serum lysozyme activity in children with autism spectrum disorder

Montiel¹,

Escalona²,

Carrero³

et al. 2018

Invest Clin

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Recent studies have suggested that Autism Spectrum Disorder (ASD) may be caused by immunological factors, particularly, abnormalities in the innate immune system. Polymorphonuclear neutrophils (PMNs) play a critical role in host defense against infection and in the resolution of inflammation, hence lysozyme plays an important role in the innate immune system. The aim of this study was to determine the in vitro phagocytic and lysozyme activity in individuals with ASD, by biochemical and immunological techniques. Results indicate that there are no qualitative or quantitative differences in the in vitro phagocytic activity between ASD and typical development (TD) subjects. The ability to reduce Nitro Blue Tetrazolium by PMNs was compared and there were no significant differences between groups. The percentage of yeasts eliminated by phagocytosis at 20 minutes of exposure to PMNs, was lower in ASD children compared to TD children; however, no significant differences were observed between the percentages of cells eliminated after 5 and 20 minutes of treatment in both populations (p>0.05). Serum lysozyme basal levels were greater in children with ASD probably due to a compensatory state because of the immunological deregulation reported for this neurodevelopmental disorder.

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“…Blood monocyte count was measured using sample-stained glass in optical microscope. Metabolic blood monocyte activity was assessed using spontaneous and stimulated nitro-blue tetrazolium test (NBT; Freeman and King 1972;Gordon et al 1973;Müller et al 1981;Hart et al 1999;Mosser and Zhang 2011;Kazanci et al 2017). Blood monocyte phagocytic activity was assessed through the expression of FcγRIII (Fc γ receptor III, CD16) using the ingestion and attachment of the opsonized sheep erythrocytes (Mosser and Zhang 2011).…”

Section: Assessment Of the Immune System Statementioning

confidence: 99%

Difference between the biologic and chronologic age as an individualized indicator for the skin care intensity selection: skin topography and immune system state studies, parameter correlations with age difference

Sukhovei¹,

Kostolomova²,

Unger³

et al. 2019

biomed dermatol

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Background: Present research addresses the issue of skin aging and corresponding skin treatment individualization. Particular research question was on the developing of simplified criterion supporting patient-specific decision on the necessity and intensity of skin treatment. Basing on the published results and a wide pool of experimental data, we have formulated a hypothesis that a difference between biologic and chronologic age can be used as an express criterion of skin aging. Methods: In present paper, we report the results of studies with 80 volunteers between 15 and 65 years of age, linking parameters reflecting immune state, skin state, and topography to the difference between biologic and chronologic age. Facial skin topography, skin moisture, sebum level, and skin elasticity were studied using commercial devices. Blood immunology studies were performed using venous blood samples. Correlations between all measured parameters and age difference were calculated. Also, cross correlations between skin cell profile and blood immune profile parameters, and skin roughness parameters were calculated. Results: Age dependencies of the blood immunological parameters on the biologic and chronologic age difference are less pronounced as compared to the changes in skin cell profile parameters. However, the changes in the tendencies when biologic age becomes equal to chronologic one are visible for all studied parameters. All measured skin roughness parameters show correlations with age difference, but average skin roughness and depth of the deepest profile valley have the largest correlation coefficient values. Many of the measured skin cell profile and blood immunology parameters show strong correlations with average skin roughness and deepest profile valley, with some of the coefficients exceeding 0.5-0.6. Conclusions: Basing on own experiments and published research results, it is possible to suggest using the difference between calculated biologic age and chronologic age as an individualized criterion supporting decisions on skin treatment strategy. Further research involving larger numbers of participants and aiming on optimizing the expressions for calculating biologic age could lead to reliable and easily available express criterion supporting the decision making for an individualized skin treatment.

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Nitroblue-tetrazolium test for the functional evaluation of phagocytic cells: A critical analysis of the methodology

Cited by 10 publications

References 15 publications

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

Increased serum lysozyme activity in children with autism spectrum disorder

Difference between the biologic and chronologic age as an individualized indicator for the skin care intensity selection: skin topography and immune system state studies, parameter correlations with age difference

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