Nitrofurazone Removal from Water Enhanced by Coupling Photocatalysis and Biodegradation

Smułek, Wojciech; Bielan, Zuzanna; Pacholak, Amanda; Zdarta, Agata; Zgoła‐Grześkowiak, Agnieszka; Zielińska‐Jurek, Anna; Kaczorek, Ewa

doi:10.3390/ijms22042186

Cited by 17 publications

(6 citation statements)

References 33 publications

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“…For the toxicity of the extracts against the tested strain, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was implemented according to Smułek et al [ 24 ]. The changes in bacteria total cell membrane permeability were investigated with the crystal violet method described by Pacholak et al [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Extracts from Frangula alnus Mill. and Their Effects on Environmental and Probiotic Bacteria

Kledecka

Siejak²,

Pratap-Singh³

et al. 2022

Plants

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The bark of Frangula alnus Mill (FAM), the so-called alder buckthorn, has been widely investigated for its medicinal properties, especially its laxative effects and the bioactive properties of the plant material extract. Still, there is no wider study devoted to its antibacterial properties. This is important in the context of its impact on probiotic gut bacteria. The aim of the research was to recognize the effect of FAM extract on bacterial cells, and to determine how the bioactive properties and composition of the extract are influenced by the type of solvent used for the extraction. To find the most suitable conditions for the FAM extraction, we used four solvent solutions with different polarities, including water, methanol, ethanol, and isopropanol. We assessed the quality and composition of the extracts with spectral analysis, using spectrophotometric (FTIR, UV-Vis) and chromatographic methods (GC-MS). Finally, we analyzed the extractant impact of the extracts on the selected bacterial cells. The results showed that the chemical diversity of the extracts increased with the increase in solvent polarity, in which the abundance of frangulin, the main bioactive compound in buckthorn bark, was confirmed. Pseudomonas fluorescens ATCC 17400 was particularly sensitive to the action of extracts, whereas other strains of the Pseudomonas genus showed practically no adverse effects. Ethanolic extracts had the strongest effect on most of the selected bacteria strains. We found that the probiotic Lactobacillus strain, which represents intestinal microflora, has no direct effect on probiotic microorganisms. The research shown FAM extracts can be safe for probiotic bacteria present in human gut microflora. Moreover, the study indicated that contact with the extracts may reduce the total permeability of the bacterial membranes. This opens up the possibility of using FAM extracts as a factor regulating transport into cells, which may be used to support the action of other bioactive substances.

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Section: Methodsmentioning

confidence: 99%

Extracts from Frangula alnus Mill. and Their Effects on Environmental and Probiotic Bacteria

Kledecka

Siejak²,

Pratap-Singh³

et al. 2022

Plants

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“…The first assay included the measurement of cell metabolic activity using the MTT indicator, similar to what is described in [ 57 ]. Further analyses included a study of changes in cell membrane permeability.…”

Section: Methodsmentioning

confidence: 99%

Multidisciplinary Studies of Folk Medicine “Five Thieves’ Oil” (Olejek Pięciu Złodziei) Components

et al. 2021

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To meet the growing interest in natural antibacterial agents, we evaluated the physicochemical and biological properties of the folk medicine known as “five thieves’ oil” (Polish name: olejek pięciu złodziei). Five thieves’ oil consists of a mixture of five oils: rosemary, lemon, clove, eucalyptus, and cinnamon. In this study, we performed gas chromatography, FTIR, and UV–vis spectroscopic analysis, as well as L-a-b color tests, contact angle determination, and surface tension determination. To verify its antibacterial activity, the metabolic activity and changes in cell membrane permeability of bacteria of the genus Pseudomonas were studied. As a result, it was found that among the constituent oils, the oils of clove and cinnamon were the least volatile and, at the same time, had the strongest antibacterial activity. However, a mix of all the oils also showed comparable activity, which was even more pronounced for the oils after 4 weeks of aging. This effect can be linked to the high content of terpene derivatives such as eugenol and cinnamaldehyde, which can cause changes in bacterial membrane permeability, affecting cell activity and survival. This study is the first to characterize the constituents of the popular folk medicine five thieves’ oil, confirming and explaining its strong antibacterial activity, thus constituting a significant contribution to contemporary health education.

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“…A separate issue is the bioavailability considered in the context of bioremediation of persistent organic pollutants (POPs), such as pharmaceuticals (including nitrofuran antibiotics), pesticides, and petroleum hydrocarbons. Most of the literature on their bioremediation focuses on the metabolism of these compounds, although many studies have indicated that it is the bioavailability that may in many cases determine the effectiveness of the biodegradation process [ 31 , 32 , 33 , 34 ].…”

Section: Significance Of Bioavailabilitymentioning

confidence: 99%

Factors Influencing the Bioavailability of Organic Molecules to Bacterial Cells—A Mini-Review

Smułek

Kaczorek

2022

Molecules

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The bioavailability of organic compounds to bacterial cells is crucial for their vital activities. This includes both compounds that are desirable to the cells (e.g., sources of energy, carbon, nitrogen, and other nutrients) and undesirable compounds that are toxic to the cells. For this reason, bioavailability is an issue of great importance in many areas of human activity that are related to bacteria, e.g., biotechnological production, bioremediation of organic pollutants, and the use of antibiotics. This article proposes a classification of factors determining bioavailability, dividing them into factors at the physicochemical level (i.e., those related to the solubility of a chemical compound and its transport in aqueous solution) and factors at the microbiological level (i.e., those related to adsorption on the cell surface and those related to transport into the cell). Awareness of the importance of and the mechanisms governing each of the factors described allows their use to change bioavailability in the desired direction.

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Nitrofurazone Removal from Water Enhanced by Coupling Photocatalysis and Biodegradation

Cited by 17 publications

References 33 publications

Extracts from Frangula alnus Mill. and Their Effects on Environmental and Probiotic Bacteria

Extracts from Frangula alnus Mill. and Their Effects on Environmental and Probiotic Bacteria

Multidisciplinary Studies of Folk Medicine “Five Thieves’ Oil” (Olejek Pięciu Złodziei) Components

Factors Influencing the Bioavailability of Organic Molecules to Bacterial Cells—A Mini-Review

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