Nitrogen and Signaling

Krapp, Anne; Ferrario-Méry, Sylvie; Touraine, Bruno

doi:10.1007/0-306-48138-3_13

Cited by 9 publications

(5 citation statements)

References 148 publications

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“…Recently, it has been recognized that cellular carbon and nitrogen metabolism must be tightly coordinated (Zheng, 2009 ; Nunes-Nesi et al, 2010 ; Bao et al, 2014 , 2015b ). Maintaining a coordination of carbon-nitrogen metabolism and an appropriate balance of carbohydrates to nitrogen metabolites, also referred to as the “carbon/nitrogen balance,” is important for plant growth, development, and yield production (Coruzzi and Zhou, 2001 ; Krapp et al, 2002 ; Martin et al, 2002 ; Krapp and Truong, 2005 ; Zheng, 2009 ; Nunes-Nesi et al, 2010 ). Our previous study also indicated the importance of carbon-nitrogen metabolic balance in rice growth and development (Bao et al, 2014 , 2015a , b ).…”

Section: Introductionmentioning

confidence: 99%

A Natural Light/Dark Cycle Regulation of Carbon-Nitrogen Metabolism and Gene Expression in Rice Shoots

Liang

Ding

et al. 2016

Front. Plant Sci.

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Light and temperature are two particularly important environmental cues for plant survival. Carbon and nitrogen are two essential macronutrients required for plant growth and development, and cellular carbon and nitrogen metabolism must be tightly coordinated. In order to understand how the natural light/dark cycle regulates carbon and nitrogen metabolism in rice plants, we analyzed the photosynthesis, key carbon-nitrogen metabolites, and enzyme activities, and differentially expressed genes and miRNAs involved in the carbon and nitrogen metabolic pathway in rice shoots at the following times: 2:00, 6:00, 10:00, 14:00, 18:00, and 22:00. Our results indicated that more CO2 was fixed into carbohydrates by a high net photosynthetic rate, respiratory rate, and stomatal conductance in the daytime. Although high levels of the nitrate reductase activity, free ammonium and carbohydrates were exhibited in the daytime, the protein synthesis was not significantly facilitated by the light and temperature. In mRNA sequencing, the carbon and nitrogen metabolism-related differentially expressed genes were obtained, which could be divided into eight groups: photosynthesis, TCA cycle, sugar transport, sugar metabolism, nitrogen transport, nitrogen reduction, amino acid metabolism, and nitrogen regulation. Additionally, a total of 78,306 alternative splicing events have been identified, which primarily belong to alternative 5′ donor sites, alternative 3′ acceptor sites, intron retention, and exon skipping. In sRNA sequencing, four carbon and nitrogen metabolism-related miRNAs (osa-miR1440b, osa-miR2876-5p, osa-miR1877 and osa-miR5799) were determined to be regulated by natural light/dark cycle. The expression level analysis showed that the four carbon and nitrogen metabolism-related miRNAs negatively regulated their target genes. These results may provide a good strategy to study how natural light/dark cycle regulates carbon and nitrogen metabolism to ensure plant growth and development.

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Section: Introductionmentioning

confidence: 99%

A Natural Light/Dark Cycle Regulation of Carbon-Nitrogen Metabolism and Gene Expression in Rice Shoots

Liang

Ding

et al. 2016

Front. Plant Sci.

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“…Recently, it has been recognized that cellular C and N metabolism must be tightly coordinated. Maintaining a coordination of carbon-nitrogen metabolism and an appropriate balance of carbohydrates to nitrogen metabolites, which also is referred to as the “C/N balance”, is important for plant growth, development and yield production [ 5 , 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance

Bao

Zhao

Ding

et al. 2015

IJMS

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Glutamine synthetase 2 (GS2) is a key enzyme involved in the ammonium metabolism in plant leaves. In our previous study, we obtained GS2-cosuppressed plants, which displayed a normal growth phenotype at the seedling stage, while at the tillering stage they showed a chlorosis phenotype. In this study, to investigate the chlorosis mechanism, we systematically analyzed the plant growth, carbon-nitrogen metabolism and gene expressions between the GS2-cosuppressed rice and wild-type plants. The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon/nitrogen ratio in the stems. Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage. The analysis of the metabolic profile showed a significant increase of sugars and organic acids. Additionally, gene expression patterns were different in root and leaf of GS2-cosuppressed plants between the seedling and tillering stage. These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth.

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“…As we known, both carbon and nitrogen nutrients are essential for various cellular functions. Recently, cellular carbon and nitrogen metabolism was demonstrated to be tightly coordinated [ 8 , 9 , 10 , 11 , 56 ]. In addition to independent utilization, the coordination and optimal functioning of the metabolic pathways governing nitrogen and carbon assimilation in plants were demonstrated to be critical for plant growth and, ultimately, biomass accumulation and yield production [ 10 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, cellular carbon and nitrogen metabolism was demonstrated to be tightly coordinated [ 8 , 9 , 10 , 11 , 56 ]. In addition to independent utilization, the coordination and optimal functioning of the metabolic pathways governing nitrogen and carbon assimilation in plants were demonstrated to be critical for plant growth and, ultimately, biomass accumulation and yield production [ 10 , 56 ]. Maintaining an appropriate balance or carbohydrate to nitrogen metabolite ratio in the cell, which is referred to as the “carbon/nitrogen balance”, is extremely important for regulating plant growth, development and production yield [ 8 , 9 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpressing of OsAMT1-3, a High Affinity Ammonium Transporter Gene, Modifies Rice Growth and Carbon-Nitrogen Metabolic Status

Bao

Liang

Zhao

et al. 2015

IJMS

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AMT1-3 encodes the high affinity NH4+ transporter in rice roots and is predominantly expressed under nitrogen starvation. In order to evaluate the effect of AMT1-3 gene on rice growth, nitrogen absorption and metabolism, we generated AMT1-3-overexpressing plants and analyzed the growth phenotype, yield, carbon and nitrogen metabolic status, and gene expression profiles. Although AMT1-3 mRNA accumulated in transgenic plants, these plants displayed significant decreases in growth when compared to the wild-type plants. The nitrogen uptake assay using a 15N tracer revealed poor nitrogen uptake ability in AMT1-3-overexpressing plants. We found significant decreases in AMT1-3-overexpressing plant leaf carbon and nitrogen content accompanied with a higher leaf C/N ratio. Significant changes in soluble proteins and carbohydrates were also observed in AMT1-3-overexpressing plants. In addition, metabolite profile analysis demonstrated significant changes in individual sugars, organic acids and free amino acids. Gene expression analysis revealed distinct expression patterns of genes that participate in carbon and nitrogen metabolism. Additionally, the correlation between the metabolites and gene expression patterns was consistent in AMT1-3-overexpressing plants under both low and high nitrogen growth conditions. Therefore, we hypothesized that the carbon and nitrogen metabolic imbalance caused by AMT1-3 overexpressing attributed to the poor growth and yield of transgenic plants.

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Nitrogen and Signaling

Cited by 9 publications

References 148 publications

A Natural Light/Dark Cycle Regulation of Carbon-Nitrogen Metabolism and Gene Expression in Rice Shoots

A Natural Light/Dark Cycle Regulation of Carbon-Nitrogen Metabolism and Gene Expression in Rice Shoots

The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance

Overexpressing of OsAMT1-3, a High Affinity Ammonium Transporter Gene, Modifies Rice Growth and Carbon-Nitrogen Metabolic Status

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