Nitrogen and Sulfur Codoped Reduced Graphene Oxide as a General Platform for Rapid and Sensitive Fluorescent Detection of Biological Species

Chen, Lu; Song, Liping; Zhang, Yichi; Wang, Ping; Xiao, Zhizhong; Guo, Yu‐Guo; Cao, Fuyang

doi:10.1021/acsami.6b01030

Cited by 56 publications

(27 citation statements)

References 40 publications

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“…In another study, using the fact that doping allows one to enhance the electrical properties of 2DMs, N,S-codoped rGO was exploited as a quenching platform for the simultaneous detection of hepatitis B virus and HIV DNA targets. 170 The sp 2 -hybridized carbon atoms of rGO could be activated thanks to the doping, which improved the capacity of rGO as an electron/energy acceptor.…”

Section: ■ Detection Of Viruses and Proteins Released By Infected Org...mentioning

confidence: 99%

Two-Dimensional Material-Based Biosensors for Virus Detection

2020

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Viral infections are one of the major causes of mortality and economic losses worldwide. Consequently, efficient virus detection methods are crucial to determine the infection prevalence. However, most detection methods face challenges related to false-negative or falsepositive results, long response times, high costs, and/or the need for specialized equipment and staff. Such issues can be overcome by access to low-cost and fast response point-of-care detection systems, and two-dimensional materials (2DMs) can play a critical role in this regard. Indeed, the unique and tunable physicochemical properties of 2DMs provide many advantages for developing biosensors for viral infections with high sensitivity and selectivity. Fast, accurate, and reliable detection, even at early infection stages by the virus, can be potentially enabled by highly accessible surface interactions between the 2DMs and the analytes. High selectivity can be obtained by functionalization of the 2DMs with antibodies, nucleic acids, proteins, peptides or aptamers, allowing for specific binding to a particular virus, viral fingerprints or proteins released by the host organism. Multiplexed detection and discrimination between different virus strains are also feasible. In this review, we present a comprehensive overview of the major advances of 2DMbased biosensors for the detection of viruses. We describe the main factors governing the efficient interactions between viruses and 2DMs, making them ideal candidates for the detection of viral infections. We also critically detail their advantages and drawbacks, providing insights for the development of future biosensors for virus detection. Lastly, we provide suggestions to stimulate research in the fast expanding field of 2DMs that could help in designing advanced systems for preventing virus-related pandemics.

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Section: ■ Detection Of Viruses and Proteins Released By Infected Org...mentioning

confidence: 99%

Two-Dimensional Material-Based Biosensors for Virus Detection

2020

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“…Then, a large amount of thionine molecules were adsorbed onto the rGO surface, via π-π stacking, as an excellent electrochemical probe. Graphene or graphene oxide-based two-dimensional materials have been widely explored for application in biosensor and biomedicine, owing to their fascinating electronic, thermal, mechanical, and chemical properties (Dong et al, 2012 ; Chen et al, 2016 ; Cao et al, 2018 ; Campos et al, 2019 ). The obtained ZrO 2 -rGO-Thi nanocomposite was used as an integral multifunctional sensing element for DNA recognition, signal amplification, and reporting.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Chen

Liu

et al. 2020

Front. Chem.

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In this work, a simple but sensitive electrochemical DNA biosensor for nucleic acid detection was developed by taking advantage of exonuclease (Exo) I-assisted cleavage for background reduction and zirconia-reduced graphene oxide-thionine (ZrO 2-rGO-Thi) nanocomposite for integral DNA recognition, signal amplification, and reporting. The ZrO 2-rGO nanocomposite was obtained by a one-step hydrothermal synthesis method. Then, thionine was adsorbed onto the rGO surface, via π-π stacking, as an excellent electrochemical probe. The biosensor fabrication is very simple, with probe DNA immobilization and hybridization recognition with the target nucleic acid. Then, the ZrO 2-rGO-Thi nanocomposite was captured onto an electrode via the multicoordinative interaction of ZrO 2 with the phosphate group on the DNA skeleton. The adsorbed abundant thionine molecules onto the ZrO 2-rGO nanocomposite facilitated an amplified electrochemical response related with the target DNA. Since upon the interaction of the ZrO 2-rGO-Thi nanocomposite with the probe DNA an immobilized electrode may also occur, an Exo I-assisted cleavage was combined to remove the unhybridized probe DNA for background reduction. With the current proposed strategy, the target DNA related with P53 gene could be sensitively assayed, with a wide linear detection range from 100 fM to 10 nM and an attractive low detection limit of 24 fM. Also, the developed DNA biosensor could differentiate the mismatched targets from complementary target DNA. Therefore, it offers a simple but effective biosensor fabrication strategy and is anticipated to show potential for applications in bioanalysis and medical diagnosis.

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“…On the other hand, Chen et al has argued that although graphene oxide is a good luminescence quencher, it still suffers in real‐world systems from drawbacks such as relatively long assay times, synthetic complexity, and poor detection limits. They reasoned that these drawbacks can be caused by either the insulating nature of GO that limits electron transfer efficiency or nonspecific fouling of the GO surface in complex matrices.…”

Section: Design and Application Of Mab Constructsmentioning

confidence: 99%

Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics

Moutsiopoulou

Broyles

Dikici

et al. 2019

Small

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The ability to monitor types, concentrations, and activities of different biomolecules is essential to obtain information about the molecular processes within cells. Successful monitoring requires a sensitive and selective tool that can respond to these molecular changes. Molecular aptamer beacon (MAB) is a molecular imaging and detection tool that enables visualization of small or large molecules by combining the selectivity and sensitivity of molecular beacon and aptamer technologies. MAB design leverages structure switching and specific recognition to yield an optical on/off switch in the presence of the target. Various donor–quencher pairs such as fluorescent dyes, quantum dots, carbon‐based materials, and metallic nanoparticles have been employed in the design of MABs. In this work, the diverse biomedical applications of MAB technology are focused on. Different conjugation strategies for the energy donor–acceptor pairs are addressed, and the overall sensitivities of each detection system are discussed. The future potential of this technology in the fields of biomedical research and diagnostics is also highlighted.

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Nitrogen and Sulfur Codoped Reduced Graphene Oxide as a General Platform for Rapid and Sensitive Fluorescent Detection of Biological Species

Cited by 56 publications

References 40 publications

Two-Dimensional Material-Based Biosensors for Virus Detection

Two-Dimensional Material-Based Biosensors for Virus Detection

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics

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