Nitrogen Balance Studies with Dogs on Casein or Methionine-Supplemented Casein

Arnold, Aaron; Schad, Jessy S.

doi:10.1093/jn/53.2.265

Cited by 11 publications

(3 citation statements)

References 9 publications

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“…This prevented an excess of substrates for taurine synthesis that might overwhelm the effects of fibers on taurine metabolism studied. Twelve percent protein is higher than the minimum requirement of protein for maintenance of dogs described in National Research Council [ 17 ] and 0.35 % of sulfur amino acid concentration in the diets is within the range of total sulfur amino acid requirement (0.2–0.4 % of diets) for maintenance of adult dogs as determined by short-term nitrogen balance experiments [ 20 – 22 ]. Ten percent full-fat RB was used for the RB diet and the amounts of the fibers used in the other two diets were formulated, by calculation, to have the same amount of total dietary fiber (TDF) as that in the RB diet.…”

Section: Methodsmentioning

confidence: 99%

Dietary beet pulp decreases taurine status in dogs fed low protein diet

Fascetti

2016

J Anim Sci Technol

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BackgroundIt is known that large dogs who are fed lamb and rice diets are at increased risk to develop taurine-deficiency-induced dilated cardiomyopathy. Since dogs obligatorily conjugate bile acids (BA) with taurine, we determined whether rice bran (RB) or other fibers (cellulose; CL, beet pulp; BP) would affect BA excretion and/or the taurine status of dogs.ResultsEighteen medium/large mixed-breed dogs were given purified diets containing CL, BP, or RB for 12 weeks. Taurine concentrations in plasma and whole blood were significantly decreased at week 12. The BP group, compared to the CL or RB groups, showed significantly lower taurine concentrations in plasma (6.5 ± 0.5 vs 20.4 ± 3.9 and 13.1 ± 2.0 μmol/L, respectively, P < 0.01, mean ± SEM) and in whole blood (79 ± 10 vs 143 ± 14 and 127 ± 14 μmol/L, respectively, P < 0.01), lower apparent protein digestibility (81.9 ± 0.6 vs 88.8 ± 0.6 and 88.1 ± 1.2 %, respectively, P < 0.01), and higher BA excretions (5.6 ± 0.1 vs 3.4 ± 0.5 and 3.4 ± 0.4 μmol/g feces, respectively, P < 0.05) at week 12.ConclusionsThese results do not support the hypothesis that RB is likely to be a primary cause of lamb meal and rice diets, increasing the risk of taurine deficiency in large dogs. However these indicate that BP may contribute to a decrease taurine status in dogs by increasing excretion of fecal BA and decreasing protein digestibility, thus decreasing the bioavailability of sulfur amino acids, the precursors of taurine.

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Section: Methodsmentioning

confidence: 99%

Dietary beet pulp decreases taurine status in dogs fed low protein diet

Fascetti

2016

J Anim Sci Technol

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“…The lower values reported here reflect the differences in methodology and objectives between this short-term study and previous studies used to inform the NRC guidelines. For example, previous studies investigated responses to methionine supplementation in low-protein diets (33)(34)(35)(36) and estimated total SAA as approximately 0•5-1 g/4184 kJ. One long-term study (15) found no adverse effects when beagles were fed a diet containing total SAA of 1•3 g/4184 kJ for 4 years.…”

Section: Discussionmentioning

confidence: 99%

Short-term determination and long-term evaluation of the dietary methionine requirement in adult dogs

Harrison¹,

Thomas²,

Gilham³

et al. 2020

Br J Nutr

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Methionine, an essential sulphur-containing amino acid (SAA), plays an integral role in many metabolic processes. Evidence for the methionine requirements of adult dogs is limited, and we employed the indicator amino acid oxidation (IAAO) method to estimate dietary methionine requirements in Labrador retrievers (n 21). Using semi-purified diets, the mean requirement was 0·55 (95 % CI 0·41, 0·71) g/4184 kJ. In a subsequent parallel design study, three groups of adult Labrador retrievers (n 52) were fed semi-purified diets with 0·55 g/4184 kJ (test diet 1), 0·71 g/4184 kJ (test diet 2) or 1·37 g/4184 kJ (control diet) of methionine for 32 weeks to assess the long-term consequences of feeding. The total SAA content (2·68 g/4184 kJ) was maintained through dietary supplementation of cystine. Plasma methionine did not decrease in test group and increased significantly on test diet 1 in weeks 8 and 16 compared with control. Reducing dietary methionine did not have a significant effect on whole blood, plasma or urinary taurine or plasma N-terminal pro B-type natriuretic peptide. Significant effects in both test diets were observed for cholesterol, betaine and dimethylglycine. In conclusion, feeding methionine at the IAAO-estimated mean was sufficient to maintain plasma methionine over 32 weeks when total SAA was maintained. However, choline oxidation may have increased to support plasma methionine and have additional consequences for lipid metabolism. While the IAAO can be employed to assess essential amino acid requirements, such as methionine in the dog using semi-purified diets, further work is required to establish safe levels for commercial diet formats.

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“…[13][14][15] The current National Research Council (NRC) recommends a minimum protein requirement of 80 g of crude protein per kg diet in foods with an energy density of 4.0 kilocalories (kcal) ME/g, when proteins that are of high quality (both bioavailable and with the correct amounts of the essential amino acids) are fed. However, differences in the protein sources, energy densities, and amino acid ratios of the experimental diets have led to a great deal of confusion regarding this requirement.…”

Section: Dogsmentioning

confidence: 99%

Protein Requirements

Case¹

2011

Canine and Feline Nutrition

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Nitrogen Balance Studies with Dogs on Casein or Methionine-Supplemented Casein

Cited by 11 publications

References 9 publications

Dietary beet pulp decreases taurine status in dogs fed low protein diet

Dietary beet pulp decreases taurine status in dogs fed low protein diet

Short-term determination and long-term evaluation of the dietary methionine requirement in adult dogs

Protein Requirements

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