Nitrogen Compounds in Fermented Cigar Leaves

The Chemical Constituents Of Tobacco And Tobacco Smoke

“…Asparagine has been isolated from Burley tobacco, where it was found in appreciable quantity even after long storage, 4.4 g. being obtained from 200 g. of tobacco (113). Glutamine and asparagine content varies with the variety, and the relatively large amounts found in shade-grown tobacco were not paralleled in Pennsylvania Seed leaf, where little was found at the corresponding stage of processing (79).…”

“…There appeared to be little change in alkaloid content during aging but fermentation proceeded at the expense of the alkaloids, which were transformed into other products, some having been identified: namely, cotinine, oxynicotine, 3-pyridyl propyl ketone, 3-pyridyl methyl ketone, nicotinamide, N-methylnicotinamide, and nicotinic acid (81). Myosmine (78,126) and 2,3'-dipyridyl (321) have been found in tobacco, and a base resembling metanicotine was isolated from a nicotine-free variety (375).…”

Microbial Degradation of Nicotine-N′-oxide I Degradation Products

“…Arthrobacter globi/ormis JTS-0006 (Nox 2) which most actively degraded the oxide was employed in the experiment, since the metabolic products of all the strains isolated were similar on paperchromatogram. Culture medium reported by Wad a et al, 11) containing 2g of nicotine-N'-oxide or nicotine; 1 g, KHzPO. ; 0.5 g, MgSO.· 7H 2 0; 0.3 ml of 1 % FeS04 solution; 0.3 ml of 1 % CaClz solution; trace of MnSO.…”

Analytical Studies on Harvested Tobacco Leaves

“…Oxynicotine Nitrogen. Oxynicotine is a major component (29) of the transformation products of nicotine that have been identified in fermented samples of tobacco. In fermented Pennsylvania tobacco, for example, the quantities of oxynicotine nitro- c Measured in 0.25.V aqueous HC1 solutions in cells of 1-cm.…”

“…A XIONG the chemical changes that occur during the industrial processing of cigar tobacco leaves, the conversion of the nitrogenous leaf components plays a significant role, both qualitatively and quantitatively (28,24). In previous communications from this laboratory (26-28, 80) attention was drawn to some formerly unknown chemical changes of this kind, such as the transformation of nicotine into various other pyridine derivatives, and the change of some water-soluble nitrogenous leaf constituents into water-insoluble products (25).…”