Nitrogen deficiency in barley (<i>Hordeum vulgare</i>) seedlings induces molecular and metabolic adjustments that trigger aphid resistance

Comadira, Gloria; Rasool, Brwa; Karpińska, Barbara; Morris, Jenny; Verrall, Susan; Hedley, Pete E.; Foyer, Christine H.; Hancock, Robert D.

doi:10.1093/jxb/erv276

Cited by 65 publications

(52 citation statements)

References 71 publications

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“…RNA from each of three replicates was hybridized to a custom‐design Agilent barley 60K microarray (Comadira et al . ). The microarray experimental design and dataset can be accessed at ArrayExpress (https://www.ebi.ac.uk/arrayexpress; accession # E‐MTAB‐5133).…”

Section: Methodsmentioning

confidence: 97%

“…RNA quality was assessed using the Agilent 2100 Bioanalyzer prior to microarray processing (Agilent Technologies, Santa Clara, CA, USA). RNA from each of three replicates was hybridized to a custom-design Agilent barley 60K microarray (Comadira et al 2015). The microarray experimental design and dataset can be accessed at ArrayExpress (https://www.ebi.ac.uk/arrayexpress; accession # E-MTAB-5133).…”

Section: Barley Transcriptome Analysesmentioning

confidence: 99%

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Barley transcriptome analyses upon interaction with different aphid species identify thionins contributing to resistance

Escudero-Martinez

Morris

Hedley

et al. 2017

Plant Cell & Environment

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Aphids are phloem‐feeding insects that cause yield loss on a wide range of crops, including cereals such as barley. Whilst most aphid species are limited to one or few host species, some are able to reproduce on many plants belonging to different families. Interestingly, aphid probing behaviour can be observed on both host and non‐host species, indicating that interactions take place at the molecular level that may impact host range. Here, we aimed to gain insight into the interaction of barley with aphid species differing in their ability to infest this crop by analysing transcriptional responses. Firstly, we determined colonization efficiency, settlement and probing behaviour for the aphid species Rhopalosiphum padi, Myzus persicae and Myzus cerasi, which defined host, poor‐host and non‐host interactions, respectively. Analyses of barley transcriptional responses revealed gene sets differentially regulated upon the different barley–aphid interactions and showed that the poor‐host interaction with M. persicae resulted in the strongest regulation of genes. Interestingly, we identified several thionin genes strongly up‐regulated upon interaction with M. persicae, and to a lesser extent upon R. padi interaction. Ectopic expression of two of these genes in Nicotiana benthamiana reduced host susceptibility to M. persicae, indicating that thionins contribute to defences against aphids.

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Section: Methodsmentioning

confidence: 97%

Section: Barley Transcriptome Analysesmentioning

confidence: 99%

Barley transcriptome analyses upon interaction with different aphid species identify thionins contributing to resistance

Escudero-Martinez

Morris

Hedley

et al. 2017

Plant Cell & Environment

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“…Numerous studies have shown that high-level N fertilizer application to crops can influence plant-insect interactions, and potentially increase growth, food consumption, survival, reproductive rates and population densities of insect herbivores (Mattson, 1980; Moon et al, 1995; Brodbeck et al, 2001; Khan and Port, 2008). Conversely, a deficiency of N may alter plant metabolism and trigger insect resistance (Comadira et al, 2015). It has been demonstrated that the increasing populations of major insect pests of rice, including planthoppers, leaffolders, and stem borers, are closely related to the long-term excessive application of N fertilizers in most of the rice growing areas in Asia (Lu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Interactions between Nitrogen and Silicon in Rice and Their Effects on Resistance toward the Brown Planthopper Nilaparvata lugens

Baerson

et al. 2017

Front. Plant Sci.

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Nitrogen (N) and silicon (Si) are two important nutritional elements required for plant growth, and both impact host plant resistance toward insect herbivores. The interaction between the two elements may therefore play a significant role in determining host plant resistance. We investigated this interaction in rice (Oryza sativa L.) and its effect on resistance to the herbivore brown planthopper Nilaparvata lugens (BPH). Our results indicate that high-level (5.76 mM) N fertilization reduced Si accumulation in rice leaves, and furthermore, this decrease was likely due to decreased expression of Si transporters OsLsi1 and OsLsi2. Conversely, reduced N accumulation was observed at high N fertilization levels when Si was exogenously provided, and this was associated with down-regulation of OsAMT1;1 and OsGS1;1, which are involved in ammonium uptake and assimilation, respectively. Under lower N fertilization levels (0.72 and/or 1.44 mM), Si amendment resulted in increased OsNRT1:1, OsGS2, OsFd-GOGAT, OsNADH-GOGAT2, and OsGDH2 expression. Additionally, bioassays revealed that high N fertilization level significantly decreased rice resistance to BPH, and the opposite effect was observed when Si was provided. These results provide additional insight into the antagonistic interaction between Si and N accumulation in rice, and the effects on plant growth and susceptibility to herbivores.

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“…Soluble fertilizer, mainly nitrogen, may also affect the susceptibility of plants to insect pests by altering the nutrient levels in plant tissue or tissue fractions. For example, nitrogen fertilizer increases free amino N in several crops [162]. This biochemical imbalance affects both herbivore damage and the ability of a plant to recover from herbivory [163].…”

Section: Tropical Environment and Conventional Versus Organic Agriculmentioning

confidence: 99%

“…The use of residues such as wood ash, biofertilizer, and powdered rocks may be another low cost alternative to increase soil fertility without affecting the imbalance of nutrients [162]. Biofertilizer consists of liquid fermented manure that can be produced at a local farm, and it has been identified as an alternative to chemical fertilizer to increase soil fertility and crop production in sustainable farming [163].…”

Section: Alternative Local Resources For Organic Soil Managementmentioning

confidence: 99%

Improving Farming Practices for Sustainable Soil Use in the Humid Tropics and Rainforest Ecosystem Health

et al. 2016

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Abstract:Unsustainable farming practices such as shifting cultivation and slash-and-burn agriculture in the humid tropics threaten the preservation of the rainforest and the health of the local and global environment. In weathered soils prone to cohesion in humid tropic due to low Fe and carbon content and the enormous amounts of P that can be adsorbed, sustainable soil use is heavily dependent on the availability and efficient use of nutrients. This paper reviews the literature in the field and provides some insights about sustainable soil use in the humid tropics, mainly for the Brazilian Amazonia region. Careful management of organic matter and physical and chemical indicators is necessary to enhance root growth and nutrient uptake. To improve the rootability of the arable layer, a combination of gypsum with continuous mulching to increase the labile organic matter fraction responsible for the formation of a short-lived structure important for root growth is recommended, rather than tillage. Unlike mulching, mechanical disturbance via ploughing of Amazonian soils causes very rapid and permanent soil organic matter losses and often results in permanent recompaction and land degradation or anthropic savannization; thus, it should be avoided. Unlike in other regions, like southeast Brazil, saturating the soil solely with inorganic potassium and nitrogen soluble fertilizers is not recommended. Nutrient retention in the root zone can be enhanced if nutrients are added in a slow-release form and if biologically mediated processes are used for nutrient release, as occurs in green manure. Therefore, an alternative that favors using local resources to increase the supply of nutrients and offset processes that impair the efficiency of nutrient use must be pursued.

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Nitrogen deficiency in barley (Hordeum vulgare) seedlings induces molecular and metabolic adjustments that trigger aphid resistance

Abstract: HighlightNitrogen deficiency induces extensive metabolic adjustment in barley leaves mediated largely by sugar signalling and receptor-like kinase cascades that overlap with biotic stress pathways to induce aphid resistance.

Cited by 65 publications

References 71 publications

Barley transcriptome analyses upon interaction with different aphid species identify thionins contributing to resistance

Barley transcriptome analyses upon interaction with different aphid species identify thionins contributing to resistance

Interactions between Nitrogen and Silicon in Rice and Their Effects on Resistance toward the Brown Planthopper Nilaparvata lugens

Improving Farming Practices for Sustainable Soil Use in the Humid Tropics and Rainforest Ecosystem Health

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