Nitrogen determination by SEM‐EDS and elemental analysis

Gazulla, M. F.; Rodrigo, Marta; Blasco, E.; Orduña, M.

doi:10.1002/xrs.2490

Cited by 45 publications

(28 citation statements)

References 17 publications

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“…Further peaks were found for iron ( K α , K β and L I–III ) as well as for oxygen ( K α ) and nitrogen ( K α ). For nitrogen a detection is known to be difficult in general13 and actually in both measurements only very small signals for nitrogen were detected. However, a clear enhancement of the signal intensity of nitrogen was observed for the bulk part of the particle, while coincidently the signal intensity of oxygen was markedly reduced.…”

Section: Resultsmentioning

confidence: 99%

A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments

Kim

Tullman‐Ercek

2014

Biotechnology Journal

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Bacterial microcompartments (MCPs) are protein-based organelles that have been suggested as scaffolds for creating in vivo nanobioreactors. One of the key steps towards engineering MCPs is to understand and maximize the encapsulation of enzymes into the lumen of the MCP. However, there are currently no high-throughput methods for investigating protein encapsulation. Here, we describe the development of a rapid in vivo assay for quantifying the relative amount of protein encapsulated within MCPs based on fluorescence. In this assay, we fuse a degradation peptide to a MCP-targeted fluorescence reporter and use flow cytometry to measure overall fluorescence from the encapsulated, protected reporter protein. Using this assay, we characterized various MCP-targeting signal sequence mutants for their ability to encapsulate proteins and identified mutants that encapsulate a greater amount of protein than the wild type signal sequence. This assay is a powerful tool for reporting protein encapsulation and is an important step towards encapsulating metabolic enzymes into MCPs for synthetic biochemical pathways.

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Section: Resultsmentioning

confidence: 99%

A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments

Kim

Tullman‐Ercek

2014

Biotechnology Journal

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“…Nitrogen cannot be measured like the other elements due to matrix effect, which makes it impossible to construct a calibration curve that correlates the signal directly with the nitrogen content for any type of sample [ 72 ]. However, following Gazulla et al [ 73 ], a rough estimation of the N content of the samples is reported in Table 4 .…”

Section: Resultsmentioning

confidence: 99%

A Combined Approach to Assess the Microbial Contamination of the Archimedes Palimpsest

et al. 2014

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A combined approach, using molecular and microscopic techniques, was used to identify the microbiota associated with the Archimedes Palimpsest, an unusual parchment manuscript. SEM analyses revealed the microbial damage to the collagen fibers and the presence of characteristic cell chains typical of filamentous bacteria and fungal spores. Molecular analysis confirmed a homogeneous bacterial community colonizing the manuscript. The phyla Proteobacteria and Actinobacteria were associated with this ancient parchment; the sequences were most related to uncultured clones detected in the human skin microbiome and in ephitelium, and to cultivated species of the genera Acinetobacter and Nocardiopsis. Nevertheless, a great variation was observed among the different sampled areas indicating fungal diversity. Blumeria spp. dominated in the healthy areas of the parchment while degraded areas showed disparate fungal communities, with dominant members of the genera Mucor and Cladosporium. In addition, the quantification of the β-actin gene by real-time PCR analyses (qPCR) revealed a higher fungal abundance on degraded areas than on the healthy ones.

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“…But no N peak was observed in the spectrograms of the two amino acids. These observations indicated the capability of the EDS in detecting N atom; and the failure in N detection in our SEM-EDS was more due to the complicated sample matrix as the matrix exerts significant effects on the detection of N by SEM-EDS (Gazulla et al, 2013). Hou et al (2017) examined protein (bovine serum albumin, BSA) fouling control during membrane distillation process.…”

Section: Eds Analysis (Surface Composition)mentioning

confidence: 87%

Surface Characterization of Cottonseed Meal Products by SEM, SEM-EDS, XRD and XPS Analysis

He¹,

Cheng²,

Olanya³

et al. 2017

JMSR

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The utilization of cottonseed meal products as valuable industrial materials needs to be exploited. We have recently produced water-washed cottonseed meal, total cottonseed protein, sequentially extracted water-and alkali-soluble proteins, and two residues after the total and sequential protein extractions at a pilot scale. In this work, the surface characteristics of the six cottonseed meal products were examined by scanning electron microscopy (SEM), scanning electron microscopy-energy dispersive spectrometer (SEM-EDS), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results showed that the surface properties of the six products differed from those of a commercial soy protein flour examined comparatively in this work. The compact morphology and relative-high N composition were observed in all three protein products, with greater similarity between the total protein and alkali-soluble protein. The surfaces of the two residue products were more porous with polysaccharide features. Washed cottonseed meal possessed the surface features similar to those of the residues. In the meantime, the N-associated functional groups were under-represented in the surfaces of all samples, compared to their bulk composition. Information derived from this work increased the understanding of the surface functional properties of cottonseed meal products, which would benefit their practical utilization.

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Nitrogen determination by SEM‐EDS and elemental analysis

Cited by 45 publications

References 17 publications

A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments

A rapid flow cytometry assay for the relative quantification of protein encapsulation into bacterial microcompartments

A Combined Approach to Assess the Microbial Contamination of the Archimedes Palimpsest

Surface Characterization of Cottonseed Meal Products by SEM, SEM-EDS, XRD and XPS Analysis

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