Nitrogen Fixation by White Lupin under Phosphorus Deficiency

Schulze, Joachim; Temple, Glena G.; Temple, Stephen J.; Beschow, Heidrun; Vance, Carroll P.

doi:10.1093/aob/mcl154

Cited by 192 publications

(143 citation statements)

References 50 publications

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“…No visible anthocyanin accumulated in PdL, nor did we observe any other differences in the growth of P i -sufficient and P i -deficient aerial tissues. These results are consistent with previous studies, which showed that P i -sufficient and P i -deficient plants at 25 DAE exhibit differences in P i concentrations but show no change in overall growth responses (Schulze et al, 2006). Our analyses comparing P i -sufficient and P i -deficient transcript expression patterns at 16 DAE provide insight into gene expression profiles as plants experience P i deficiency.…”

supporting

confidence: 92%

An RNA-Seq Transcriptome Analysis of Orthophosphate-Deficient White Lupin Reveals Novel Insights into Phosphorus Acclimation in Plants

O’Rourke

Yang

Miller³

et al. 2012

Plant Physiology

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188

184

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Phosphorus, in its orthophosphate form (P i ), is one of the most limiting macronutrients in soils for plant growth and development. However, the whole-genome molecular mechanisms contributing to plant acclimation to P i deficiency remain largely unknown. White lupin (Lupinus albus) has evolved unique adaptations for growth in P i -deficient soils, including the development of cluster roots to increase root surface area. In this study, we utilized RNA-Seq technology to assess global gene expression in white lupin cluster roots, normal roots, and leaves in response to P i supply. We de novo assembled 277,224,180 Illumina reads from 12 complementary DNA libraries to build what is to our knowledge the first white lupin gene index (LAGI 1.0). This index contains 125,821 unique sequences with an average length of 1,155 bp. Of these sequences, 50,734 were transcriptionally active (reads per kilobase per million reads $ 3), representing approximately 7.8% of the white lupin genome, using the predicted genome size of Lupinus angustifolius as a reference. We identified a total of 2,128 sequences differentially expressed in response to P i deficiency with a 2-fold or greater change and P # 0.05. Twelve sequences were consistently differentially expressed due to P i deficiency stress in three species, Arabidopsis (Arabidopsis thaliana), potato (Solanum tuberosum), and white lupin, making them ideal candidates to monitor the P i status of plants. Additionally, classic physiological experiments were coupled with RNA-Seq data to examine the role of cytokinin and gibberellic acid in P i deficiency-induced cluster root development. This global gene expression analysis provides new insights into the biochemical and molecular mechanisms involved in the acclimation to P i deficiency.

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supporting

confidence: 92%

An RNA-Seq Transcriptome Analysis of Orthophosphate-Deficient White Lupin Reveals Novel Insights into Phosphorus Acclimation in Plants

O’Rourke

Yang

Miller³

et al. 2012

Plant Physiology

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188

184

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“…Most studies on the effect of P limitation on N 2 -fixation have largely been confined to model legumes such as Lupinus albus (Schulze et al, 2006, Thuynsma et al, 2013 and Medicago truncatula (Tang et al, 2001, Sulieman et al, 2013. Despite the high legume diversity found on the P-poor soils of the CFR (Goldblatt and Manning 2002), little is known about the functional mechanisms which affect N nutrition within the nodules of indigenous legumes.…”

Section: Introductionmentioning

confidence: 99%

Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels

Vardien

Mesjasz‐Przybyłowicz

Przybyłowicz

et al. 2014

Journal of Plant Physiology

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Legumes have the unique ability to fix atmospheric nitrogen (N 2 ) via symbiotic bacteria in their nodules but depend heavily on phosphorus (P), which affects nodulation, and the carbon costs and energy costs of N 2 fixation. Consequently, legumes growing in nutrient-poor ecosystems (e.g. sandstone derived soils) have to enhance P recycling and/ or acquisition in order to maintain N 2 fixation. In this study, we investigated the flexibility of P recycling and distribution within the nodules and their effect on N nutrition in Virgilia divaricata Adamson, Fabaceae, an indigenous legume in the Cape Floristic Region of South Africa. Specifically, we assessed tissue elemental localization using micro-particle induced x-ray emission 1 (PIXE), measured N fixation using nutrient concentrations derived from inductively coupled mass spectrometry (ICP-MS), calculated nutrient costs, and determined P recycling from enzyme activity assays. Morphological and physiological features characteristic of adaptation to P-deprivation were observed for V. divaricata. Decreased plant growth and nodule production with parallel increased root: shoot ratios are some of the plastic features exhibited in response to P deficiency. Plants resupplied with P resembled those supplied with optimal P levels in terms of growth and nutrient acquisition. Under low P conditions, plants maintained an increase in N 2 -fixing efficiency despite lower levels of orthophosphate (Pi) in the nodules.This can be attributed to two factors: i) an increase in Fe concentration under low P, and ii) greater APase activity in both the roots and nodules under low P. These findings suggest that V. divaricata is well-adapted to acquire N under P deficiency, owing to the plasticity of its nodule physiology.

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“…The nitrogen fixing systems require more phosphorus (P) than non-N 2 fixing systems, because the process consumes large amounts of energy, and energy-generating metabolism strongly depends upon the availability of P (Schulze et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

EFFECT OF RESIDUAL ROCK PHOSPHATE AND BIOFERTILIZERS (RHIZOBIUM AND BIOPHOSPHATE) AND THEIR COMBINATIONS ON THE PRODUCTIVITY OF Stylosanthes guianensis CIAT 184

Amakali¹,

Soetrisno²,

Budhi³

2013

BuletinPeternak

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The purpose of this research was to evaluate the agronomic effectiveness of rock phosphate, biological fertilizers (Rhizobium and bio-phosphate) and their combinations on dry matter (DM) production, nutrient content and in vitro dry matter digestibility (IVDMD) of Stylosanthes guianensis CIAT 184. This research was conducted at Forage and Pasture Laboratory, Universitas Gadjah Mada, Yogyakarta, Indonesia from February -October 2010. A strip plot design which consisted of two fertilizer factors with three (3) replicates was used. The first factor was the horizontal factor and consisted of four levels of biological fertilizers (Rhizobium and bio-phosphate) namely: M 0 = control, M 1 = Rhizobium (0.5 g/plot), M 2 = bio-phosphate (0.6 g/plot) and M 3 = combinations of Rhizobium and bio-phosphate. The second factor was the vertical factor and consisted of three levels of rock phosphate, namely: P 0 = control, P 1 = 250 kg/ha (32.5 P kg/ha) and P 2 = 500 kg/ha (65 P kg/ha). Defoliation was carried out every three months for 9 months. Results of the study showed that there was no significant difference in DM production (kg/ha) amongst treatments. Although the current research showed no significance difference amongs the treatments, DM production increased in the 2 nd harvest (12.3%) and 4 th harvest (7.1%) between P 0 and P 1 . Combinations treatment P 1 M 2 (21073.63 kg/ha) had the highest average DM production amongs treatments. Statistical analysis followed by Duncan's new Multiple Range Test (DMRT), showed that rock phosphate can significantly (P<0.05) increase fiber production of Stylosanthes guianensis. It showed that treatment P 1 increased crude fiber production by 12.2% from 5864, 47 kg/ha to 6580,19 kg/ha. In addition, tests further revealed that there was a difference between M 1 and M 2 , but had no effect on M 0 . There was no significant effect on production (kg/ha) of crude protein, phosphorus, nitrogen free extract (NFE) and ash. In conclusion, treatments with rock phosphate and bio-fertilizers tend to increase productivity of Stylosanthes guianensis CIAT 184.

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Nitrogen Fixation by White Lupin under Phosphorus Deficiency

Abstract: Enhanced nodulation in cluster root zones and increased potential for organic acid production in root nodules appear to contribute to white lupin's resilience to P-deficiency.

Cited by 192 publications

References 50 publications

An RNA-Seq Transcriptome Analysis of Orthophosphate-Deficient White Lupin Reveals Novel Insights into Phosphorus Acclimation in Plants

An RNA-Seq Transcriptome Analysis of Orthophosphate-Deficient White Lupin Reveals Novel Insights into Phosphorus Acclimation in Plants

Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels

EFFECT OF RESIDUAL ROCK PHOSPHATE AND BIOFERTILIZERS (RHIZOBIUM AND BIOPHOSPHATE) AND THEIR COMBINATIONS ON THE PRODUCTIVITY OF Stylosanthes guianensis CIAT 184

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