“…Efforts to estimate the rate of fixation have been limited because the rate of fixation is low and (or) methods are too insensitive to detect small changes in nitrogen content. The methods used include total nitrogen determinations (Kjeldahl nitrogen) (Jaiyebo and Moore, 1963;Moore, 1963;Moore and Abaelu, 1959;Olsen, 1932;Olson, 1958;Parker, 1957;Stevenson, 1958Stevenson, , 1959, lysimeter determinations (Chapman, Liebig, et al, 1949;Gel'tser, 1961;Smith, 1944), N215 studies (Delwiche and Wijler, 1956;Stewart, 1965), and acetylene-ethylene studies (Hardy, Holsten, et al, 1968;Stewart, Fitzgerald, et al, 1967). Since the Kjeldahl determination is too imprecise to detect small changes, large numbers of samples must be assayed and given statistical analysis.…”