Nitrogen Fixation Through Genetic Engineering: A Future Systemic Approach of Nitrogen Fixation

Bahuguna, Vivekanand; Bhatt, Gaurav; Maikhuri, Richa; Chandra, Deepika

doi:10.1007/978-981-15-9758-9_5

Cited by 1 publication

(1 citation statement)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Engineered microbes promise to make substantial impacts on pressing societal challenges. Biotechnologies deployed in environments of biotic and abiotic complexity, such as cell-based therapeutics in the human gut (1), nitrogen fixation in the plant rhizosphere (2,3), and plastic (4) and plant biomass bioconversion (5,6) in industrial bioreactors (7,8), will be central in addressing grand challenges to promote human health, reverse carbon emissions, recycle mixed plastic waste, remediate contaminated soils, and achieve sustainable economies. Many natural microbes can thrive in these environments but lack the functions required to address these aims.…”

Section: Introductionmentioning

confidence: 99%

High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

et al. 2023

View full text Add to dashboard Cite

Efficient genome engineering is critical to understand and use microbial functions. Despite recent development of tools such as CRISPR-Cas gene editing, efficient integration of exogenous DNA with well-characterized functions remains limited to model bacteria. Here, we describe serine recombinase–assisted genome engineering, or SAGE, an easy-to-use, highly efficient, and extensible technology that enables selection marker–free, site-specific genome integration of up to 10 DNA constructs, often with efficiency on par with or superior to replicating plasmids. SAGE uses no replicating plasmids and thus lacks the host range limitations of other genome engineering technologies. We demonstrate the value of SAGE by characterizing genome integration efficiency in five bacteria that span multiple taxonomy groups and biotechnology applications and by identifying more than 95 heterologous promoters in each host with consistent transcription across environmental and genetic contexts. We anticipate that SAGE will rapidly expand the number of industrial and environmental bacteria compatible with high-throughput genetics and synthetic biology.

show abstract