Nitrogen removal performance and microbial characteristics during simultaneous chemical phosphorus removal process using Fe3+

Huang, Xiao; Yao, Kai; Yu, Jianguo; Dong, Wenyi; Zhao, Zilong

doi:10.1016/j.biortech.2022.127972

Cited by 47 publications

(6 citation statements)

References 36 publications

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“…NO 3 – -N was converted to NO 2 – -N via EC1.9.6.1 and EC1.7.5.1, and the abundance of both enzymes was significantly enhanced in the presence of 10 mg/L Fe 3+ . The addition of Fe 3+ inhibited both EC1.7.2.1 and EC1.7.2.5, thereby contributing to a reduction in the conversion of NO 2 – -N to NO and N 2 O ( Huang et al, 2022 ). In contrast, both the conversion of N 2 O to N 2 and N fixation were enhanced in response to the addition of Fe 3+ , whereas N 2 O was transformed to NH 4 + -N in the presence of EC1.7.7.1, EC1.7.1.15, and EC1.7.2.2.…”

Section: Resultsmentioning

confidence: 99%

“…A single Fe 3+ ion can produce an average of six adsorption vacancies, thereby facilitating bonding with other substances, and consequently resulting in flocculation in response to the effect of adsorption bridging (Zhang et al, 2021). However, 10.3389/fmicb.2023 Fe 3+ can combine with PO 4 3− and HPO 4 2− to form precipitates, thereby facilitating the chemical removal of P, and thus the effective elimination of P from wastewater (Huang et al, 2022).…”

Section: Effect Of Fe 3+ On Nutrient Removalmentioning

confidence: 99%

“…Furthermore, Fe 3+ has been found to modify the N cycle and promotes or inhibits the activity of certain microorganisms. Conversely, an increase in the abundance of certain nitrification-related enzymes or microbes has been observed in the presence of Fe 3+ , whereas this contributes to a reduction in denitrification efficiency ( Huang et al, 2022 ). The assembly of microbial communities is associated with two important and complementary processes, namely deterministic processes based on the ecological niche theory, and stochastic processes based on the neutral theory ( He et al, 2022 ; Lu M. et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Zhong

Pang³

et al. 2023

Front. Microbiol.

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In this study, the influence of Fe3+ on N removal, microbial assembly, and species interactions in a biofilm system was determined. The results showed that maximum efficiencies of ammonia nitrogen (NH4+-N), total nitrogen (TN), phosphorus (P), and chemical oxygen demand (COD) removal were achieved using 10 mg/L Fe3+, reaching values of 100, 78.85, 100, and 95.8%, respectively, whereas at concentrations of 15 and 30 mg/L Fe3+ suppressed the removal of NH4+-N, TN, and COD. In terms of absolute abundance, the expression of bacterial amoA, narG, nirK, and napA was maximal in the presence of 10 mg/L Fe3+ (9.18 × 105, 8.58 × 108, 1.09 × 108, and 1.07 × 109 copies/g dry weight, respectively). Irrespective of Fe3+ concentrations, the P removal efficiency remained at almost 100%. Candidatus_Competibacter (10.26–23.32%) was identified as the most abundant bacterial genus within the system. Determinism (50%) and stochasticity (50%) contributed equally to microbial community assembly. Co-occurrence network analysis revealed that in the presence of Fe3+, 60.94% of OTUs in the biofilm system exhibited positive interactions, whereas 39.06% exhibited negative interactions. Within the OTU-based co-occurrence network, fourteen species were identified as key microbes. The stability of the system was found to be predominantly shaped by microbial cooperation, complemented by competition for resources or niche incompatibility. The results of this study suggested that during chemical P removal in wastewater treatment plants using biofilm methods, the concentration of supplemental Fe3+ should be maintained at 10 mg/L, which would not only contribute to P elimination, but also enhance N and COD removal.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Fe 3+ On Nutrient Removalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Zhong

Pang³

et al. 2023

Front. Microbiol.

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“…Moreover, the abundance of genera Denitratisoma (r = 0.95, p < 0.001), Bdellovibrio (r = 0.86, p < 0.05), and env.OPS_17 (r = 0.92, p < 0.001) showed a positive correlation with PFOA concentration. Similarly, the abundance of OLB8 sharply increased from 0.96 ± 0.11% in phase I to11.37 ± 0.24% in Phase V. Previous studies have demonstrated that Denitratisoma [54], Bdellovibrio [55], env.OPS_17 [56], and OLB8 [57] are involved in denitrification processes. This suggested that these denitrifying genera were potential PFOA-resistant members.…”

Section: Effects Of Pfoa Stress On the Microbial Community Structuresmentioning

confidence: 99%

Insights into the Responses of the Partial Denitrification Process to Elevated Perfluorooctanoic Acid Stress: Performance, EPS Characteristic and Microbial Community

Zhang,

Feng

et al. 2023

Water

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This study aimed at investigating the potential impacts of perfluorooctanoic acid (PFOA) exposure on the partial denitrification (PD) system. Our results indicated that nitrite accumulation rates were significantly decreased to 67.94 ± 1.25%–69.52 ± 3.13% after long-term PFOA exposure (0.5–20 mg/L), while the nitrate transformation ratio was slightly impacted. The PFOA removal efficiency gradually decreased from 67.42 ± 3.39% to 6.56 ± 5.25% with an increasing PFOA dosage, indicating that the main PFOA removal pathway was biosorption. The average EPS contents increased by two folds, which suggested that exposure to PFOA significantly stimulated EPS secretion. Excitation emission matrix analysis revealed that PFOA exposure promoted the secretion of tryptophan protein-like, humic acid-like, and aromatic protein II-like substances, which may act as a protective barrier against PFOA toxicity. Moreover, significant changes in characteristic peaks after PFOA exposure were shown as indicated by Fourier transform infrared spectroscopy. High-throughput sequencing suggested that PFOA significantly decreased bacterial richness and increased evenness, indicating that toxicity effects of PFOA were more pronounced for abundant species (e.g., Thauera) than rare species. Thauera was the most dominant genus responsible for nitrite accumulation, whose abundance significantly decreased from 35.99 ± 2.67% to 18.60 ± 2.18% after PFOA exposure. In comparison, the abundances of common denitrifiers, such as Denitratisoma, Bdellovibrio, and OLB8, significantly increased, suggesting that these genera were potential PFOA-resistant bacteria. This study presents new insights into the effect of PFOA on a PD system.

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“…Thauera belongs to denitrifying bacteria, which is an important function in the denitrification and degradation of pollutants in the system, and also has the function of EPS secretion. Saccharimonadales (39.692%) and Defluviicoccus (8.259%) were more abundant in A4, Saccharimonadales survive in an anaerobic environment and participate in denitrification (Huang et al, 2022). Defluviicoccus has a phosphorus removal effect.…”

Section: Community Structure Analysis At the Genus Levelmentioning

confidence: 99%

Changes in microbial community during hydrolyzed sludge reduction

Liu,

Wu,

et al. 2023

Front. Microbiol.

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In this study, the effects of different enzymes (lysozyme, α-amylase and neutral protease) on sludge hydrolysis efficiency and microbial community in sequencing batch reactor (SBR) were introduced. The results showed that the hydrolysis efficiencies of the three enzymes were 48.5, 22.5 and 31%, respectively, compared with the accumulated sludge discharge of the blank control group. However, it has varying degrees of impact on the effluent quality, and the denitrification and phosphorus removal effect of the system deteriorates. The lysozyme that achieves the optimal sludge hydrolysis effect of 48.5% has the greatest impact on the chemical oxygen demand (COD), total nitrogen (TN), and nitrate nitrogen (NO3−-N) of the effluent. The sludge samples of the control group and the groups supplemented with different enzyme preparations were subjected to high-throughput sequencing. It was found that the number of OTUs (Operational Taxonomic Units) of the samples was lysozyme > α-amylase > blank control > neutral protease. Moreover, the abundance grade curve of the sludge samples supplemented with lysozyme and α-amylase was smoother, and the community richness and diversity were improved by lysozyme and α-amylase. The species diversity of the sludge supplemented with lysozyme and neutral protease was great, and the community succession was obvious. The introduction of enzymes did not change the main microbial communities of the sludge, which were mainly Proteobacteria, Actinobacteria and Bacteroidetes. The effects of three enzyme preparations on sludge reduction and microbial diversity during pilot operation were analyzed, the gap in microbial research was filled, which provided theoretical value for the practical operation of enzymatic sludge reduction.

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Nitrogen removal performance and microbial characteristics during simultaneous chemical phosphorus removal process using Fe3+

Cited by 47 publications

References 36 publications

Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Effect of Fe3+ on the nutrient removal performance and microbial community in a biofilm system

Insights into the Responses of the Partial Denitrification Process to Elevated Perfluorooctanoic Acid Stress: Performance, EPS Characteristic and Microbial Community

Changes in microbial community during hydrolyzed sludge reduction

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