Nitroso-R Salt As a Sensitive Spectrophotometric Reagent For the Determination of Vitamin C

Arya, Sunil; Mahajan, Meenakshi

doi:10.1080/00032719708001764

Cited by 14 publications

(7 citation statements)

References 11 publications

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“…The method using 2-OCHT determines ascorbic acid up to 12 µg ml -1 (ε=1.49×10 4 ), but is interfered with by metal ions such as Cu(II), Co(II), Ni(II) and Pd(II), in addition to the interference caused by the oxalic acid, riboflavin, oxidants and reductants. Color-forming reactions of Fe(II) with ferrozine [117][118][119] (λ max =562 nm) in acidic solutions (pH 3 -6), TPTZ [120][121][122] (λ max =593, 595 nm), quinaldic acid in presence of pyridine 123 (λ max =380 nm), picolinic acid in presence of pyridine 124 (λ max =400 nm) and nitroso-R salt 125 (λ max =705 nm) have been used for the determination of vitamin C in a variety of samples. The reagents picolinic acid and quinaldic acid, when complexed with iron(II) in the presence of pyridine, resulted in methods used successfully in the analysis of pharmaceuticals, food products and biological samples.…”

Section: Resultsmentioning

confidence: 99%

Photometric Methods for the Determination of Vitamin C

1998

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Section: Resultsmentioning

confidence: 99%

Photometric Methods for the Determination of Vitamin C

1998

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“…The Fe(NRS) 3 3À complex was claimed to absorb strongly at 370 nm but with small contribution above 500 nm (Arya and Mahajan, 1997). It was observed that in the reaction medium An Alternative FRAP Assay Using the Fe(III)/NR Salt Complexes in which this assay was developed the absorbance values of the Fe(NRS) 3 3À complex (the reference solution) cannot be neglected.…”

Section: à Complexesmentioning

confidence: 99%

“…Fe(II) also reacts with three molecules of NRS to form an aqueous soluble complex of bright green colour, with absorption maximum at 730 nm (Oka and Miyamoto, 1955). Ascorbic acid was quantified in pharmaceutical forms due to reduction of Fe(III) to Fe(II) complexes at pH =7.0 using citrate buffer (Arya and Mahajan, 1997).…”

Section: Introductionmentioning

confidence: 99%

The Evaluation of Reduction of Fe(III) in 3‐Hydroxy‐4‐Nitroso‐2,7‐Naphthalene Disulphonic Medium as an Alternative Ferric Reducing Activity Power Assay

Souza

Moya

2014

Phytochemical Analysis

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“…The apparent molar absorptivity (ε) was calculated from the slope of the calibration graph to be 2.05 × 10 6 dm 3 mol -1 cm -1 . This procedure is much more sensitive than other spectrophotometric methods, using iron(III)-nitroso R 15 (ε = 4.5 × 10 , and is as sensitive as the Leuco Crystal Violet method (ε = 1.6 × 10 6 ). 21 The relative standard deviation (RSD) for five runs of 0.7 µg AA was 2.2%.…”

Section: Calibration Curvementioning

confidence: 99%

Spectrophotometric Determination of Ascorbic Acid with Iron(III) and p-Carboxyphenylfluorone in a Cationic Surfactant Micellar Medium

et al. 2001

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1 is an important vitamin which participates in a great variety of biological events concerning electrontransport reactions, hydroxylations, the oxidative catabolism of aromatic amino acids and so on. Numerous papers and reviews 2-5 have described the determination of AA. The methods used include titrimetric, spectrophotometric, fluorometric, electrochemical, chromatographic, kinetic and chemiluminescent procedures. Photometric methods continue to be of interest because of ease in accessibility and their quick applicability to routine analyses. The majority of these methods are based on their oxidation-reduction properties or ability to couple with diazotized aniline derivatives. Many spectrophotometric methods based on the reduction of iron(III) to iron(II) with AA, followed by complexation of reduced iron(II) with different reagents, such as 1,10-phenanthroline (phen) and 2,2′-dipyridyl, have been reported. Another fast and facile method based on a proportional decrease in the absorbance of iron(III) complexes by the addition of AA has been proposed by some authors. On the other hand, AA has several donor atoms capable of metal complex formation and binding 6,7 with zinc(II), manganese(II), cadmium(II), alkaline earth metals and so on.We have already reported 8-10 on simple and sensitive determinations of various organic compounds utilizing a mixedcomplex system among an organic compound, a metal ion and a dye. Here, we have studied the color reaction of AA based on a coupled redox-complexation reaction among iron(III), xanthene dye and AA; also, a simple and highly sensitive spectrophotometric determination of AA was investigated by using iron(III) and p-carboxyphenylfluorone (PCPF) as a dye in the presence of a cationic surfactant. The proposed method was then applied to assays in pharmaceutical preparations. Experimental Reagents and solutionsAA and dehydroascorbic acid (DHAA), analytical-grade reagents, were purchased from Wako Pure Chemical Co. Ltd., and used without further purification. The stock and working solutions of AA were freshly prepared every day. DHAA was prepared daily not more than 2 h prior to use by dissolving DHAA in water. A solution of iron(III) (5.0 × 10 -4 M, 1 M = 1 mol dm -3 ) was prepared by dissolving a calculated amount of ferric ammonium sulfate in 100 cm 3 of water containing 1 cm 3 of hydrochloric acid (HCl) and a 10-fold molar excess of sodium fluoride over iron(III). A 1.0 × 10 -3 M solution of PCPF, which had been synthesized according to a method described in the literature, 11,12 was prepared in methanol containing one drop of HCl. A 1.0 × 10 -2 M solution of ntetradecyltrimethylammonium chloride (TTAC) was prepared in water. A buffer solution of pH 8.5 was prepared by mixing 0.1 M HCl and 0.1 M 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris) solutions. All other reagents and materials were of analytical grade, and were used without further purification. Pure water was prepared by purifying deionized water with a Milli-Q Labo System just before use. ApparatusA Shimadzu (...

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Nitroso-R Salt As a Sensitive Spectrophotometric Reagent For the Determination of Vitamin C

Cited by 14 publications

References 11 publications

Photometric Methods for the Determination of Vitamin C

Photometric Methods for the Determination of Vitamin C

The Evaluation of Reduction of Fe(III) in 3‐Hydroxy‐4‐Nitroso‐2,7‐Naphthalene Disulphonic Medium as an Alternative Ferric Reducing Activity Power Assay

Spectrophotometric Determination of Ascorbic Acid with Iron(III) and p-Carboxyphenylfluorone in a Cationic Surfactant Micellar Medium

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